Hyaluronic Acid/Chitosan-g-Poly(ethylene glycol) Nanoparticles for Gene Therapy: An Application for pDNA and siRNA Delivery

2010 ◽  
Vol 27 (12) ◽  
pp. 2544-2555 ◽  
Author(s):  
Manuela Raviña ◽  
Eva Cubillo ◽  
David Olmeda ◽  
Ramón Novoa-Carballal ◽  
Eduardo Fernandez-Megia ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Sirna Delivery ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

A bioactive hyaluronic acid–based hydrogel cross-linked by Diels–Alder reaction for promoting neurite outgrowth of PC12 cells

2017 ◽  
Vol 32 (4) ◽  
pp. 382-396 ◽  
Author(s):  
Dongming Xing ◽  
Lie Ma ◽  
Changyou Gao
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Alder Reaction ◽  
Poly Ethylene Glycol ◽  
Substitution Degree ◽  
Poly Ethylene

In order to improve neurite outgrowth on the in situ formed hyaluronic acid–based hydrogel, furan and methacrylate groups were grafted on hyaluronic acid successively. Furthermore, a laminin-derived peptide CQAASIKVAV was covalently immobilized via the Michael addition. The furan- and peptide-modified hyaluronic acid was then cross-linked in situ by mixing with bismaleimide poly(ethylene glycol) at 37 °C to obtain a bioactive hyaluronic acid–based hydrogel. The hyaluronic acid derivatives were characterized by 1H NMR and Fourier transform infrared spectroscopy. The gelation, swelling, and mechanical property of the hydrogels were analyzed. The modulus of the hydrogel could be tuned by changing furan substitution degree, while the peptide concentration could be changed by the ratio of furan- and peptide-modified hyaluronic acid with hyaluronic acid–furan. In vitro culture of PC12 cells showed that the longest neurite outgrowth appeared on the hyaluronic acid–poly(ethylene glycol) hydrogel with the highest peptide content (the substitution degree of peptide in furan- and peptide-modified hyaluronic acid was 23 %) and a lower threshold modulus of 4.5 kPa. The furan and methacrylate-functionalized hyaluronic acid provides a versatile platform for diverse functionalization and can be used for modulation of other cell behaviors as well.

Copolymers of amine methacrylate with poly(ethylene glycol) as vectors for gene therapy

2001 ◽  
Vol 73 (2-3) ◽  
pp. 359-380 ◽  
Author(s):  
Uracha Rungsardthong ◽  
Mangesh Deshpande ◽  
Lindsey Bailey ◽  
M. Vamvakaki ◽  
Steven P. Armes ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Cleavable poly(ethylene glycol) branched chain-modified Antheraea pernyi silk fibroin as a gene delivery carrier

Nanomedicine ◽  
2021 ◽  
Author(s):  
Xueping Liu ◽  
Hong Luo ◽  
Longxing Niu ◽  
Yanfei Feng ◽  
Peng Pan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Ethylene Glycol ◽  
Silk Fibroin ◽  
Plasma Proteins ◽  
Kidney Cells ◽  
Poly Ethylene Glycol ◽  
Normal Cells ◽  
Antheraea Pernyi ◽  
Poly Ethylene ◽  
Branched Chain

Aim: To obtain a gene carrier that can effectively deliver loaded therapeutic genes to tumor cells, avoid toxic effects on normal cells and reduce nonspecific adsorption of plasma proteins. Methods: The conjugate of poly(ethylene glycol) (PEG) and MMP2SSP (PEG-MMP2SSP) was covalently coupled to cationized Antheraea pernyi silk fibroin (CASF) through disulfide bond exchange reaction to obtain a PEG-MMP2SSP-modified CASF (CASFMP). Results: The PEG chains were effectively cleaved from the CASFMP by MMP2. CASFMP/pDNA complexes inhibited human fibrosarcoma cell proliferation, and its cytotoxicity to human normal embryonic kidney cells was significantly lower than that of poly(ethylenimine)/pDNA after coculturing with cells for 24 h. Conclusion: CASFMP is a promising compound for use in gene therapy.

Sign in / Sign up

Export Citation Format

Share Document