Photoelectrochemical Biosensor for Sensitive Detection of Soluble CD44 Based on the Facile Construction of a Poly(ethylene glycol)/Hyaluronic Acid Hybrid Antifouling Interface

2019 ◽  
Vol 11 (27) ◽  
pp. 24764-24770 ◽  
Author(s):  
Bobo Fan ◽  
Qi Fan ◽  
Min Cui ◽  
Tingting Wu ◽  
Jinshen Wang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Sensitive Detection ◽  
Poly Ethylene Glycol ◽  
Soluble Cd44 ◽  
Photoelectrochemical Biosensor ◽  
Poly Ethylene

A bioactive hyaluronic acid–based hydrogel cross-linked by Diels–Alder reaction for promoting neurite outgrowth of PC12 cells

2017 ◽  
Vol 32 (4) ◽  
pp. 382-396 ◽  
Author(s):  
Dongming Xing ◽  
Lie Ma ◽  
Changyou Gao
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Alder Reaction ◽  
Poly Ethylene Glycol ◽  
Substitution Degree ◽  
Poly Ethylene

In order to improve neurite outgrowth on the in situ formed hyaluronic acid–based hydrogel, furan and methacrylate groups were grafted on hyaluronic acid successively. Furthermore, a laminin-derived peptide CQAASIKVAV was covalently immobilized via the Michael addition. The furan- and peptide-modified hyaluronic acid was then cross-linked in situ by mixing with bismaleimide poly(ethylene glycol) at 37 °C to obtain a bioactive hyaluronic acid–based hydrogel. The hyaluronic acid derivatives were characterized by 1H NMR and Fourier transform infrared spectroscopy. The gelation, swelling, and mechanical property of the hydrogels were analyzed. The modulus of the hydrogel could be tuned by changing furan substitution degree, while the peptide concentration could be changed by the ratio of furan- and peptide-modified hyaluronic acid with hyaluronic acid–furan. In vitro culture of PC12 cells showed that the longest neurite outgrowth appeared on the hyaluronic acid–poly(ethylene glycol) hydrogel with the highest peptide content (the substitution degree of peptide in furan- and peptide-modified hyaluronic acid was 23 %) and a lower threshold modulus of 4.5 kPa. The furan and methacrylate-functionalized hyaluronic acid provides a versatile platform for diverse functionalization and can be used for modulation of other cell behaviors as well.

Hyaluronic Acid/Chitosan-g-Poly(ethylene glycol) Nanoparticles for Gene Therapy: An Application for pDNA and siRNA Delivery

2010 ◽  
Vol 27 (12) ◽  
pp. 2544-2555 ◽  
Author(s):  
Manuela Raviña ◽  
Eva Cubillo ◽  
David Olmeda ◽  
Ramón Novoa-Carballal ◽  
Eduardo Fernandez-Megia ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Sirna Delivery ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Interpolyelectrolyte complexes based on hyaluronic acid-block-poly(ethylene glycol) and poly-l-lysine

Soft Matter ◽  
2013 ◽  
Vol 9 (16) ◽  
pp. 4297 ◽  
Author(s):  
Ramon Novoa-Carballal ◽  
Dmitry V. Pergushov ◽  
Axel H. E. Müller
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Interpolyelectrolyte Complexes ◽  
Poly Ethylene
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