Gamma Irradiation of Active Self-Healing PLGA Microspheres for Efficient Aqueous Encapsulation of Vaccine Antigens

Kashappa-Goud H. Desai; Samer Kadous; Steven P. Schwendeman

doi:10.1007/s11095-013-1019-2

Gamma Irradiation of Active Self-Healing PLGA Microspheres for Efficient Aqueous Encapsulation of Vaccine Antigens

Pharmaceutical Research ◽

10.1007/s11095-013-1019-2 ◽

2013 ◽

Vol 30 (7) ◽

pp. 1768-1778 ◽

Cited By ~ 8

Author(s):

Kashappa-Goud H. Desai ◽

Samer Kadous ◽

Steven P. Schwendeman

Keyword(s):

Gamma Irradiation ◽

Self Healing ◽

Plga Microspheres ◽

Vaccine Antigens

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Active self-healing encapsulation of vaccine antigens in PLGA microspheres

Journal of Controlled Release ◽

10.1016/j.jconrel.2012.10.012 ◽

2013 ◽

Vol 165 (1) ◽

pp. 62-74 ◽

Cited By ~ 51

Author(s):

Kashappa-Goud H. Desai ◽

Steven P. Schwendeman

Keyword(s):

Self Healing ◽

Plga Microspheres ◽

Vaccine Antigens

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Effect of Self-healing Encapsulation on the Initial Burst Release from PLGA Microspheres Containing a Long-Acting Prostacyclin Agonist, ONO-1301

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.c17-00025 ◽

2017 ◽

Vol 65 (7) ◽

pp. 653-659 ◽

Cited By ~ 6

Author(s):

Mai Hazekawa ◽

Honami Kojima ◽

Tamami Haraguchi ◽

Miyako Yoshida ◽

Takahiro Uchida

Keyword(s):

Burst Release ◽

Self Healing ◽

Plga Microspheres ◽

Initial Burst ◽

Initial Burst Release ◽

Long Acting

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Effect of Polymer Porosity on Aqueous Self-Healing Encapsulation of Proteins in PLGA Microspheres

Macromolecular Bioscience ◽

10.1002/mabi.201300323 ◽

2013 ◽

Vol 13 (12) ◽

pp. 1700-1710 ◽

Cited By ~ 16

Author(s):

Samuel E. Reinhold ◽

Steven P. Schwendeman

Keyword(s):

Self Healing ◽

Plga Microspheres

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Self-healing encapsulation and controlled release of vaccine antigens from PLGA microparticles delivered by microneedle patches

Bioengineering & Translational Medicine ◽

10.1002/btm2.10103 ◽

2018 ◽

Vol 4 (1) ◽

pp. 116-128 ◽

Cited By ~ 14

Author(s):

J. Maxwell Mazzara ◽

Lukasz J. Ochyl ◽

Justin K. Y. Hong ◽

James J. Moon ◽

Mark R. Prausnitz ◽

...

Keyword(s):

Controlled Release ◽

Self Healing ◽

Vaccine Antigens ◽

Plga Microparticles

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Protein Loading into Spongelike PLGA Microspheres

Pharmaceutics ◽

10.3390/pharmaceutics13020137 ◽

2021 ◽

Vol 13 (2) ◽

pp. 137

Author(s):

Yuyoung Kim ◽

Hongkee Sah

Keyword(s):

Tween 80 ◽

Critical Parameters ◽

Self Healing ◽

Plga Microspheres ◽

Pore Characteristics ◽

Protein Loading ◽

Loading Efficiency ◽

Model Protein ◽

Critical Process Parameters ◽

Closed Pore

A self-healing microencapsulation process involves mixing preformed porous microspheres in an aqueous solution containing the desired protein and converting them into closed-pore microspheres. Spongelike poly-d,l-lactide-co-glycolide (PLGA) microspheres are expected to be advantageous to protein loading through self-healing. This study aimed to identify and assess relevant critical parameters, using lysozyme as a model protein. Several parameters governed lysozyme loading. The pore characteristics (open-pore, closed-pore, and porosity) of the preformed microspheres substantially affected lysozyme loading efficiency. The type of surfactant present in the aqueous medium also influenced lysozyme loading efficiency. For instance, cetyltrimethylammonium bromide showing a superior wetting functionality increased the extent of lysozyme loading more than twice as compared to Tween 80. Dried preformed microspheres were commonly used before, but our study found that wet microspheres obtained at the end of the microsphere manufacturing process displayed significant advantages in lysozyme loading. Not only could an incubation time for hydrating the microspheres be shortened dramatically, but also a much more considerable amount of lysozyme was encapsulated. Interestingly, the degree of microsphere hydration determined the microstructure and morphology of closed-pore microspheres after self-healing. Understanding these critical process parameters would help tailor protein loading into spongelike PLGA microspheres in a bespoke manner.

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Autonomous self-healing polyisoprene elastomers with high modulus and good toughness based on the synergy of dynamic ionic crosslinks and highly disordered crystals

Polymer Chemistry ◽

10.1039/d0py01034k ◽

2020 ◽

Vol 11 (41) ◽

pp. 6549-6558

Author(s):

Yohei Miwa ◽

Mayu Yamada ◽

Yu Shinke ◽

Shoichi Kutsumizu

Keyword(s):

Mechanical Properties ◽

Room Temperature ◽

Self Healing ◽

High Modulus ◽

Disordered Crystals ◽

Ionic Crosslinks

We designed a novel polyisoprene elastomer with high mechanical properties and autonomous self-healing capability at room temperature facilitated by the coexistence of dynamic ionic crosslinks and crystalline components that slowly reassembled.

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