Role of jasmonate signaling in rice resistance to the leaf folder Cnaphalocrocis medinalis

Many plant pathogens suppress antimicrobial defenses using virulence factors that modulate endogenous host defenses. The Pseudomonas syringae phytotoxin coronatine (COR) is believed to promote virulence by acting as a jasmonate analog, because COR-insensitive 1 (coi1) Arabidopsis thaliana and tomato mutants are impaired in jasmonate signaling and exhibit reduced susceptibility to P. syringae. To further investigate the role of jasmonate signaling in disease development, we analyzed several jasmonate-insensitive A. thaliana mutants for susceptibility to P. syringae pv. tomato strain DC3000 and sensitivity to COR. Jasmonate-insensitive1 (jin1) mutants exhibit both reduced susceptibility to P. syringae pv. tomato DC3000 and reduced sensitivity to COR, whereas jasmonate-resistant 1 (jar1) plants exhibit wild-type responses to both COR and P. syringae pv. tomato DC3000. A jin1 jar1 double mutant does not exhibit enhanced jasmonate insensitivity, suggesting that JIN1 functions downstream of jasmonic acid-amino acid conjugates synthesized by JAR1. Reduced disease susceptibility in jin1 mutants is correlated with elevated expression of pathogenesis-related 1(PR-1) and is dependent on accumulation of salicylic acid (SA). We also show that JIN1 is required for normal P. syringae pv. tomato DC3000 symptom development through an SA-independent mechanism. Thus,P. syringae pv. tomatoDC3000 appears to utilize COR to manipulate JIN1-dependent jasmonate signaling both to suppress SA-mediated defenses and to promote symptom development.

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The role of jasmonate signaling pathway in plant’s flowering genes response to ionizing radiation

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv ◽

10.7124/visnyk.utgis.17.1.1200 ◽

2019 ◽

Vol 17 (1) ◽

pp. 45-50

Author(s):

M. V. Kryvokhyzha ◽

K. V. Krutovsky ◽

N. M. Rashydov

Keyword(s):

Arabidopsis Thaliana ◽

Ionizing Radiation ◽

Jasmonic Acid ◽

Total Dose ◽

Dose Rate ◽

Real Time Pcr ◽

Chronic Irradiation ◽

Jasmonate Signaling ◽

Flowering Genes

Aim. This study aimed to characterize the role of the jasmonate signaling pathway in flowering genes response to acute and chronic ionizing irradiation in plants. Methods. We used the wild-type Arabidopsis thaliana and jasmonic pathway defective jin mutant of Col0 ecotype in our experiments. The chronic irradiation was provided by 137СsCl with a total dose of 17 cGy and a dose rate of 6.8×10-6 cGy/s. The acute irradiation experiment was performed on 21 days old plants at the 5.0 stage (Boyes 2001) by X-rays in a total dose of 5 Gy with a dose rate of 89 cGy/s. The length of stems and leaves was measured in post-irradiation period. The molecular genetic analysis was done using real-time PCR. We determined the relative expression of key flowering genes AP1, GI, FT, CO, ACT2 with UBQ10 used as reference genes. Statistical analysis of phenotypic parameters was done using Student’s t-test in GraphPad Prism 8 software. The quantitative PCR data were analyzed in the REST 2009 software, QIAGEN. Results. The plant groups differed significantly by the stem length (p>0,05). The study revealed decreased expression of CO, GI and FT genes in jin mutants. The overexpression of AP1 in jin mutants under chronic irradiation may cause cell division errors and impact flower development. In contrast, AP1 expression in WT plants was near to normal =1 under chronic irradiation. These results suggest the involvement of the jasmonate pathway in the regulation of plants flowering during the irradiation. Сonclusion. Based on the results of our study, we hypothesize that jasmonic acid has a stabilizing effect on the rate of cell differentiation in plants under chronic irradiation. Despite the uncovered role of jasmonic acid in Arabidopsis thaliana flowering the exact mechanism of its action remains unclear and requires further investigation.Keywords: jasmonate signaling, jasmonic acid, JA, flowering, ionizing radiation, real-time PCR, relative expression.

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Virulence assay and role of Bacillus thuringiensis TS110 as biocontrol agent against the larval stages of rice leaffolder Cnaphalocrocis medinalis

Journal of Parasitic Diseases ◽

10.1007/s12639-016-0835-9 ◽

2016 ◽

Vol 41 (2) ◽

pp. 491-495

Author(s):

Tuhin Subhra Ghosh ◽

Soumendranath Chatterjee ◽

Syed Afrin Azmi ◽

Abhijit Mazumdar ◽

Tushar Kanti Dangar

Keyword(s):

Bacillus Thuringiensis ◽

Biocontrol Agent ◽

Cnaphalocrocis Medinalis ◽

Larval Stages ◽

Virulence Assay ◽

Rice Leaffolder

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Role of NINJA in root jasmonate signaling

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1307910110 ◽

2013 ◽

Vol 110 (38) ◽

pp. 15473-15478 ◽

Cited By ~ 83

Author(s):

I. F. Acosta ◽

D. Gasperini ◽

A. Chetelat ◽

S. Stolz ◽

L. Santuari ◽

...

Keyword(s):

Jasmonate Signaling

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Role of salicylate and jasmonate signaling in lipopolysaccharide-induced resistance of Arabidopsis thaliana to the phytopathogenic strain of Pseudomonas aeruginosa IMB 9096

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv ◽

10.7124/visnyk.utgis.15.1.713 ◽

2017 ◽

Vol 15 (1) ◽

pp. 58-63

Author(s):

J. V. Shilina ◽

M. I. Guscha ◽

O. S. Molozhava ◽

S. V. Litvinov ◽

A. P. Dmitriev

Keyword(s):

Pseudomonas Aeruginosa ◽

Arabidopsis Thaliana ◽

Model Systems ◽

Resistance Testing ◽

Pr Genes ◽

Bacterial Gene ◽

Signaling Systems ◽

Jasmonate Signaling ◽

Different Strains

Aim. The aim of the investigation was to study the effect of lipopolysaccharides (LPS) derived from saprophytic strains of Pseudomonas aeruginosa on the resistance to phytopathogenic strain of P. aeruginosa IMB 9096. The wild-type (Col-0) Arabidopsis thaliana plants, npr1 mutant, which lacks expression of PR-genes, NahG genotype plants, expressing the bacterial gene of NahG salicylate hydrolase, jin1 mutant, insensitive to jasmonic acid, have been used as a model systems in resistance testing. Methods. Common phytopathological methods were used. Results. Lipopolysaccharide from the saprophyte P. aeruginosa IMV 8614 strain increased the resistance of seedlings of all genotypes to infection with phytopathogenic strain P. aeruginosa IMB 9096. The most effective protection had been observed in the mutant jin1. The protective effect was also observed in jin1 after the treatment with LPS derived from the saprophyte strain P. aeruginosa IMV 8615. LPS 8615 increased the sensitivity to infection in the NahG and npr1 transgenic plants, especially in NahG. LPS from the saprophyte P. aeruginosa IMV 8616 increased resistance to P. aeruginosa IMB 9096 infection in all four A. thaliana genotypes. Conclusions. The effect of LPS derived from different strains of saprophytic bacteria can both increase and decrease the sensitivity of plants to infection with bacterial phytopathogens. The effect of LPS depends upon the bacteria strain and the functional state of the salicylate and jasmonate signaling systems in the infected plants.Keywords: Arabidopsis thaliana, Pseudomonas aeruginosa, lipopolysaccharide, induced resistanse

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Phenylalanine Suppresses Cell Death Caused by Loss of Fumarylacetoacetate Hydrolase in Arabidopsis

10.21203/rs.3.rs-1221177/v1 ◽

2022 ◽

Author(s):

Yihe Jiang ◽

Qi Zhu ◽

Hua Yang ◽

Tiantian Zhi ◽

Chunmei Ren

Keyword(s):

Cell Death ◽

Methyl Jasmonate ◽

Chlorophyll Biosynthesis ◽

Phenylpropanoid Pathway ◽

Degradation Pathway ◽

Marker Genes ◽

Jasmonate Signaling ◽

Short Day ◽

Fumarylacetoacetate Hydrolase

Abstract Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of Tyrosine (Tyr) degradation pathway essential to animals and the deficiency of FAH causes an inborn lethal disease. In plants, a role of this pathway was unknown until we found that mutation of Short-day Sensitive Cell Death1 (SSCD1), encoding Arabidopsis FAH, results in cell death under short day. Phenylalanine (Phe) could be converted to Tyr and then degraded in both animals and plants. Phe ingestion in animals worsens the disease caused by FAH defect. However, in this study we found that Phe represses cell death caused by FAH defect in plants. Phe treatment promoted chlorophyll biosynthesis and suppressed the up‑regulation of reactive oxygen species marker genes in the sscd1 mutant. Furthermore, the repression of sscd1 cell death by Phe could be reduced by α-aminooxi-β-phenylpropionic acid but increased by methyl jasmonate, which inhibits or activates Phe ammonia-lyase catalyzing the first step of phenylpropanoid pathway, respectively. In addition, we found that jasmonate signaling up‑regulates Phe ammonia-lyase 1 and mediates the methyl jasmonate enhanced repression of sscd1 cell death by Phe. These results uncovered the relation between chlorophyll biosynthesis, phenylpropanoid pathway and jasmonate signaling in regulating the cell death resulting from loss of FAH in plants.

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