Extracellular Matrix Surface Network During Plant Regeneration in Wheat Anther Culture

2005 ◽  
Vol 83 (2) ◽  
pp. 201-208 ◽  
Author(s):  
R. Konieczny ◽  
J. Bohdanowicz ◽  
A.Z. Czaplicki ◽  
L. Przywara
Keyword(s):  
Extracellular Matrix ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Matrix Surface ◽  
Surface Network

scholarly journals Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

2014 ◽  
Vol 83 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Maria Pilarska ◽  
Marzena Popielarska-Konieczna ◽  
Halina Ślesak ◽  
Małgorzata Kozieradzka-Kiszkurno ◽  
Grzegorz Góralski ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Regeneration ◽  
Vascular Bundles ◽  
Helianthus Tuberosus ◽  
Important Species ◽  
Electron Microscopies ◽  
Transmission Electron ◽  
Matrix Surface ◽  
Surface Network

<em>Helianthus tuberosus</em> is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (<strong><em>i</em></strong>) the morphogenic capacity of callus in topinambur is genotype dependent, (<strong><em>ii</em></strong>) cv. Albik of <em>H. tuberosus</em> proved recalcitrant in in vitro regeneration, and (<strong><em>iii</em></strong>) extracellular matrix surface network is not a morphogenic marker in this cultivar.

scholarly journals Are extracellular matrix surface network components involved in signalling and protective function?

2008 ◽  
Vol 3 (9) ◽  
pp. 707-709 ◽  
Author(s):  
Marzena Popielarska-Konieczna ◽  
Małgorzata Kozieradzka-Kiszkurno ◽  
Joanna Świerczyńska ◽  
Grzegorz Góralski ◽  
Halina Ślesak ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Protective Function ◽  
Matrix Surface ◽  
Surface Network

Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture

2008 ◽  
Vol 27 (7) ◽  
pp. 1137-1145 ◽  
Author(s):  
M. Popielarska-Konieczna ◽  
M. Kozieradzka-Kiszkurno ◽  
J. Świerczyńska ◽  
G. Góralski ◽  
H. Ślesak ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Callus Culture ◽  
Histochemical Analysis ◽  
Matrix Surface ◽  
Surface Network

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

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