Isopentenyl transferase gene expression offers the positive selection of marker-free transgenic plant of Kalanchoe blossfeldiana

2009 ◽  
Vol 97 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Gunaratnam Thirukkumaran ◽  
Raham Sher Khan ◽  
Dong Poh Chin ◽  
Ikuo Nakamura ◽  
Masahiro Mii
Keyword(s):  
Gene Expression ◽  
Transgenic Plant ◽  
Positive Selection ◽  
Isopentenyl Transferase ◽  
Transferase Gene ◽  
Kalanchoe Blossfeldiana ◽  
Marker Free ◽  
Kalanchoë Blossfeldiana ◽  
Selection Of

scholarly journals Selection of marker-free transgenic plants using the isopentenyl transferase gene

1997 ◽  
Vol 94 (6) ◽  
pp. 2117-2121 ◽  
Author(s):  
H. Ebinuma ◽  
K. Sugita ◽  
E. Matsunaga ◽  
M. Yamakado
Keyword(s):  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene ◽  
Marker Free ◽  
Selection Of

Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker

2010 ◽  
Vol 30 (4) ◽  
pp. 587-597 ◽  
Author(s):  
Raham Sher Khan ◽  
Valentine Otang Ntui ◽  
Dong Poh Chin ◽  
Ikuo Nakamura ◽  
Masahiro Mii
Keyword(s):  
Positive Selection ◽  
Selection Marker ◽  
Isopentenyl Transferase ◽  
Disease Resistant ◽  
Transferase Gene ◽  
Marker Free

scholarly journals Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers

2021 ◽  
Vol 12 ◽  
Author(s):  
Bruno Trevenzoli Favero ◽  
Yi Tan ◽  
Yan Lin ◽  
Hanne Bøge Hansen ◽  
Nasim Shadmani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plant Growth ◽  
Growth Habit ◽  
Open Reading Frames ◽  
Gene Copy ◽  
Binding Motif ◽  
Common Denominator ◽  
Kalanchoe Blossfeldiana ◽  
Reading Frames ◽  
Kalanchoë Blossfeldiana

Reduced growth habit is a desirable trait for ornamental potted plants and can successfully be obtained through Rhizobium rhizogenes transformation in a stable and heritable manner. Additionally, it can also be obtained by transformation with Agrobacterium tumefaciens harboring specific genes from R. rhizogenes. The bacterial T-DNA harbors four root oncogenic loci (rol) genes and 14 less known open reading frames (ORFs). The four rol genes, i.e., rolA, rolB, rolC, and rolD, are conceived as the common denominator for the compact phenotype and the other less characterized ORFs seem auxiliary but present a potential breeding target for less aberrant and/or more tailored phenotypes. In this study, Kalanchoë blossfeldiana ‘Molly’ was transformed with individual rol genes and selected ORFs in 35S overexpressing cassettes to comprehensively characterize growth traits, gene copy and expression, and ethylene tolerance of the flowers. An association of reduced growth habit, e.g. height and diameter, was observed for rolB2 and ORF14-2 when a transgene single copy and high gene expression were detected. Chlorophyll content was reduced in overexpressing lines compared to wild type (WT), except for one ΔORF13a (a truncated ORF13a, where SPXX DNA-binding motif is absent). The flower number severely decreased in the overexpressing lines compared to WT. The anthesis timing showed that WT opened the first flower at 68.9 ± 0.9 days and the overexpressing lines showed similar or up to 24 days delay in flowering. In general, a single or low relative gene copy insertion was correlated to higher gene expression, ca. 3 to 5-fold, in rolB and ΔORF13a lines, while in ORF14 such relation was not directly linked. The increased gene expression observed in rolB2 and ΔORF13a-2 contributed to reducing plant growth and a more compact habit. Tolerance of detached flowers to 0.5 μl L−1 ethylene was markedly higher for ORF14 with 66% less flower closure at day 3 compared to WT. The subcellular localization of rolC and ΔORF13a was investigated by transient expression in Nicotiana benthamiana and confocal images showed that rolC and ΔORF13a are soluble and localize in the cytoplasm being able to enter the nucleus.

A novel principle for selection of transgenic plant cells: positive selection

1996 ◽  
Vol 16 (3-4) ◽  
pp. 219-221 ◽  
Author(s):  
Morten Joersbo, T. Okkels
Keyword(s):  
Transgenic Plant ◽  
Positive Selection ◽  
Plant Cells ◽  
Selection Of

Isopentenyl transferase gene (ipt) downstream transcriptionally fused with gene expression improves the growth of transgenic plants

2009 ◽  
Vol 19 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Jian-Chun Guo ◽  
Rui-Jun Duan ◽  
Xin-Wen Hu ◽  
Kai-Mian Li ◽  
Shao-Ping Fu
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene

USING MANNOSE AS A POSITIVE SELECTION OF TRANSFORMED CARICA PAPAYA L. VAR ‘EKSOTIKA’

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Roslinda A. Razak ◽  
Rogayah Sekeli ◽  
Noor Azmi Shaharuddin ◽  
Janna Ong Abdullah
Keyword(s):  
Carbon Source ◽  
Positive Selection ◽  
Carica Papaya ◽  
Transformation Efficiency ◽  
Selection System ◽  
Phosphomannose Isomerase ◽  
Embryogenic Calli ◽  
Putative Transformants ◽  
Marker Free ◽  
Selection Of

The main objective of this study is to develop marker-free transgenic papaya plants via positive selection using phosphomannose isomerase (pmi) gene. Phosphomannose isomerase (PMI) is an enzyme that converts mannose-6-phosphate to fructose-6-phosphate, a glycolysis intermediate that supports the growth of plant cells. To establish a marker-free positive selection system using this PMI, the effect of mannose on the growth and development of embryogenic ‘Eksotika’ papaya callus was evaluated. One-month old embryogenic calli were cultured on Murashige and Skoog (MS) medium in which 60 g/L sucrose in the original recipe was replaced with different concentrations of mannose and sucrose. Mannose was supplied as the sole carbon source or in combination with sucrose at 0, 5, 10, 15, 20, 25 or 30 g/L. Embryogenic calli cultured on medium supplemented with a ratio of 0:60 g/L mannose: sucrose was used as a control. The results after six sub-cultures showed that most of the embryogenic calli transferred on media containing only mannose turned brown. Higher concentrations of mannose resulted in higher percentage of brown calli (dead). Mannose at 30 g/L mannose was found to be effective for screening transformed embryogenic calli.  Evaluation of papaya transformation efficiency using this positive selection system was pursued using 650 one-month-old embryogenic calli Agrobacterium-transformed with pNOV2819 harboring the pmi gene. Only transformed cells are capable of utilizing mannose as a carbon source to grow. After five months on mannose selection, all 67 putative transformants obtained were PCR-positives for the pmi gene.

scholarly journals Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction

2010 ◽  
Vol 27 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Gunaratnam Thirukkumarana ◽  
Valentine Otang Ntui ◽  
Raham Sher Khan ◽  
Ikuo Nakamura ◽  
Masahiro Mii
Keyword(s):  
Transgenic Plants ◽  
Hairy Root ◽  
Root Induction ◽  
Kalanchoe Blossfeldiana ◽  
Marker Free ◽  
Hairy Root Induction ◽  
Kalanchoë Blossfeldiana

A novel principle for selection of transgenic plant cells: positive selection

1996 ◽  
Vol 16 (3-4) ◽  
pp. 219-221 ◽  
Author(s):  
Morten Joersbo ◽  
Finn T. Okkels
Keyword(s):  
Transgenic Plant ◽  
Positive Selection ◽  
Plant Cells ◽  
Selection Of

Carbon dioxide uptake by succulents

1971 ◽  
Vol 49 (8) ◽  
pp. 1520-1522 ◽  
Author(s):  
M. Holdsworth
Keyword(s):  
Carbon Dioxide ◽  
Plant Sample ◽  
Carbon Dioxide Absorption ◽  
Carbon Dioxide Uptake ◽  
Kalanchoe Blossfeldiana ◽  
Succulent Plants ◽  
Kalanchoë Blossfeldiana ◽  
Selection Of

Carbon dioxide absorption by a selection of succulent plants, was followed by a modification of Tregunna's method, in which the plant sample on absorbing the carbon dioxide from an enclosed volume of air in contact with weak bicarbonate, causes the solution's pH to change. Of the 21 species tested, 11 took up carbon dioxide in the dark: Lithops turbiniformis, Zygocactus truncatus, Bryophyllum daigremontianum, Crassula multicava, Echeveria gibbiflora, Kalanchoe blossfeldiana, Sedum confusum, S. pachyphyllum, S. rubrotinctum, S. spectabile, Aloe globuligemma. The duration of uptake varied from a few hours to the whole night (and longer). Even in those other species where uptake occurred in the daytime it started and stopped at times not immediately related to the onset of light or darkness.

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