scholarly journals Assessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in Coffea arabica

2015 ◽  
Vol 122 (3) ◽  
pp. 517-531 ◽  
Author(s):  
Roberto Bobadilla Landey ◽  
Alberto Cenci ◽  
Romain Guyot ◽  
Benoît Bertrand ◽  
Frédéric Georget ◽  
...  
Keyword(s):  
Cell Culture ◽  
Somaclonal Variation ◽  
Coffea Arabica ◽  
Epigenetic Changes

scholarly journals Erratum to: Assessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in Coffea arabica

2015 ◽  
Vol 122 (3) ◽  
pp. 533-533
Author(s):  
Roberto Bobadilla Landey ◽  
Alberto Cenci ◽  
Romain Guyot ◽  
Benoît Bertrand ◽  
Frédéric Georget ◽  
...  
Keyword(s):  
Cell Culture ◽  
Somaclonal Variation ◽  
Coffea Arabica ◽  
Epigenetic Changes

Cytogenetic abnormalities of cotton somaclones from callus cultures

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 762-770 ◽  
Author(s):  
David M. Stelly ◽  
D. W. Altman ◽  
R. J. Kohel ◽  
T. S. Rangan ◽  
E. Commiskey
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Somaclonal Variation ◽  
High Frequency ◽  
Chromosomal Abnormalities ◽  
Callus Cultures ◽  
Meiotic Abnormalities ◽  
High Frequencies ◽  
Field Environment ◽  
Metaphase I

Somaclonal variation occurs among regenerants from tissue culture of many plant species. Our objective was to determine whether cytogenetic variation contributes to somaclonal variation in cotton (Gossyptum hirsutum L.,2n = 4x = 52). Of 117 somaclones of cotton regenerated from 18-month-old callus cultures of 'SJ-2' and 'SJ-5' cultivars, 35 were analyzed for meiotic abnormalities. The population of somaclones was extremely varied in phenotype, most plants being strikingly aberrant in phenotype. Fertility was generally poor: 84% failed to set bolls and only 5% set 10 or more bolls in a field environment. Only one of the somaclones (3%) formed 26 bivalents at metaphase I. Fourteen were nonsynaptic to partially synaptic at metaphase I. Synaptic abnormalities impaired fertility and precluded thorough metaphase analysis. Chromosome numbers obtained for 32 plants ranged from 49 to 53, and only 1 plant was hyperaneuploid. No plant was polyploid. Chromosomal abnormalities in plants with normal metaphase pairing included univalents, unequal bivalents, rod bivalents, trivalents, open quadrivalents, and centric fragments. Seventeen hypoaneuploid plants formed a V-shaped trivalent at metaphase I, constituting a high frequency of tertiary monosomy. The high frequencies of aneuploidy and tertiary monosomy indicate that cytogenetic anomalies are a major source of somaclonal variation in cotton. It is hypothesized that (i) primary cytogenetic events during cotton cell culture give rise to breakage – fusion – bridge (BFB) cycles, (ii) BFB cycles accrue during culture, (iii) BFB cycles cause loss of chromatin, and (iv) BFB cycles are resolved by the formation of stable tertiary chromosomes with mono-centric activity. The hypothesis accounts mechanistically for the coincidence of chromatin deficiencies and chromatin exchange involved implicitly in tertiary monosomy, as well as for the relatively high frequency of tertiary monosomy among somaclones.Key words: aneuploid, monosomic, synaptic, sterility, Gossypium.

Somaclonal variation from Sorghum bicolor (L.) Moench cell culture

1987 ◽  
Vol 9 (3) ◽  
pp. 189-196 ◽  
Author(s):  
S. Bhaskaran ◽  
R. H. Smith ◽  
S. Paliwal ◽  
K. F. Schertz
Keyword(s):  
Cell Culture ◽  
Sorghum Bicolor ◽  
Somaclonal Variation ◽  
Sorghum Bicolor L

Somatic cell culture of rice cultivars with different grain types: Somaclonal variation in some grain and quality characters

1989 ◽  
Vol 18 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Zheng Kang-Le ◽  
Zhou Zong-Ming ◽  
Wang Guo-Liang ◽  
Luo Yu-Kun ◽  
Xiong Zhen-Min
Keyword(s):  
Cell Culture ◽  
Somatic Cell ◽  
Somaclonal Variation ◽  
Rice Cultivars

scholarly journals Paraquat induces epigenetic changes by promoting histone acetylation in cell culture models of dopaminergic degeneration

2011 ◽  
Vol 32 (5) ◽  
pp. 586-595 ◽  
Author(s):  
C. Song ◽  
A. Kanthasamy ◽  
H. Jin ◽  
V. Anantharam ◽  
A.G. Kanthasamy
Keyword(s):  
Cell Culture ◽  
Histone Acetylation ◽  
Epigenetic Changes ◽  
Culture Models ◽  
Cell Culture Models ◽  
Dopaminergic Degeneration

scholarly journals Random Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid Hazelnut

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1079-1082 ◽  
Author(s):  
Mehmet Nuri Nas ◽  
Nedim Mutlu ◽  
Paul E. Read
Keyword(s):  
Somaclonal Variation ◽  
Clonal Propagation ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Epigenetic Changes ◽  
Phenotypic Analysis ◽  
Micropropagated Plants ◽  
Genetic Assessment

RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropagated plants (6-year cultures), and no somaclonal variation was detected among in vitro-propagated plants. However, polymorphism (15.6%) was detected between the parent plant and its in vitro-propagated progenies (from seedlings). These results show a good discriminatory power of RAPD to detect polymorphism between samples where it is expected, and it can be effectively used for genetic assessment of micropropagated hazelnut. No evidence of genetic or epigenetic changes was observed in long-term cultured hazelnut, and thus long-term in vitro culture of hazelnut does not seem to limit its clonal propagation.

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