Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes

Conservation translocation and reintroduction for the purpose of repopulating and reinforcing extirpated or depleted populations has been recognised as an important conservation tool, particularly for gibbon conservation in the immediate future. Feasibility assessments involving multiple factors, including taxonomic and genetic assessment of rescued and captive gibbons, are imperative prior to translocation and reintroduction programmes. In this study, we attempt to determine the subspecies and origin of captive Hylobates lar, White-handed gibbons, from Peninsular Malaysia to assist in future translocation and reintroduction programmes. A total of 12 captive and rescued H. lar samples were analysed using the control region segment of mitochondrial DNA. Sequence analyses and phylogenetic trees constructed using neighbour-joining, maximum likelihood, Bayesian inference, and network methods congruently differentiate all 12 captive individuals used in this study from other H. lar subspecies suggesting that these individuals belong to the H. lar lar subspecies. In addition, two populations of H. l. lar were observed: (1) a southern population consisting of all 12 individuals from Peninsular Malaysia, and (2) a possible northern population represented by three individuals (from previous studies), which might have originated from the region between the Isthmus of Kra, Surat Thani-Krabi depression, and Kangar-Pattani. Our findings suggest that the complete control region segment can be used to determine the subspecies and origin of captive H. lar.

Genetic Analysis of Fruit Yield and its Components in Bottle Gourd [Lagenaria siceraria (Mol.) Standl.]

Background: The choice of parents to be incorporated in hybridization is a crucial step for breeders for the improvement of complex quantitative characters, such as fruit yield and its components. It requires extensive and detailed genetic assessment of existing germplasm and newly developed promising lines. The magnitude and type of gene action serves as criteria for selection of parents, which after hybridization are likely to produce the best recombinants for desirable traits. Methods: Forty five bottle gourd genotypes comprising 36 hybrids and 9 parents were evaluated in four different environments. Genetic components of variation were estimated for 12 different characters including fruit yield per plant. Result: The additive as well as dominant components were significant for fruit yield per plant and its components, revealing equal importance of both additive as well as non-additive gene effects. However, fruit yield per plant is under the control of dominance variance. Over dominance type of gene action, considerable degree of gene symmetry over all the loci, excess of dominant alleles in parents and high narrow sense heritability was found for most of the traits studied.

MRI CNS Atrophy Pattern and the Etiologies of Progressive Ataxias

MRI shows in-vivo the three archetypal patterns of CNS volume loss underlying progressive ataxias, namely spinal atrophy (SA), cortical cerebellar atrophy (CCA) and olivopontocerebellar atrophy (OPCA). The MRI-based CNS atrophy pattern was reviewed in 128 progressive ataxias. A CNS atrophy pattern was identified in 91 conditions: SA in Freidreich’s ataxia, CCA in 5 acquired and 72 (24 dominant, 47 recessive,1 X-linked) inherited ataxias, OPCA in Multi-System Atrophy and 12 (9 dominant, 2 recessive,1 X-linked) inherited ataxias. The MRI-based CNS atrophy pattern may be useful for genetic assessment, identification of shared cellular targets, and repurposing therapies or enlargement of drugs indications in progressive ataxias.

Genetic Structure and Gene Flow in Eastern Grey Kangaroos in an Isolated Conservation Reserve

Dispersal is a key process for population persistence, particularly in fragmented landscapes. Connectivity between habitat fragments can be easily estimated by quantifying gene flow among subpopulations. However, the focus in ecological research has been on endangered species, typically excluding species that are not of current conservation concern. Consequently, our current understanding of the behaviour and persistence of many species is incomplete. A case in point is the eastern grey kangaroo (Macropus giganteus), an Australian herbivore that is subjected to considerable harvesting and population control efforts. In this study, we used non-invasive genetic sampling of eastern grey kangaroos within and outside of the Mourachan Conservation Property to assess functional connectivity. In total, we genotyped 232 samples collected from 17 locations at 20 microsatellite loci. The clustering algorithm indicated the presence of two clusters, with some overlap between the groups within and outside of the reserve. This genetic assessment should be repeated in 10–15 years to observe changes in population structure and gene flow over time, monitoring the potential impact of the planned exclusion fencing around the reserve.

Noninvasive Genetic Assessment Is an Effective Wildlife Research Tool When Compared with Other Approaches

Wildlife research has been indispensable for increasing our insight into ecosystem functioning as well as for designing effective conservation measures under the currently high rates of biodiversity loss. Genetic and genomic analyses might be able to yield the same information on, e.g., population size, health, or diet composition as other wildlife research methods, and even provide additional data that would not be possible to obtain by alternative means. Moreover, if DNA is collected non-invasively, this technique has only minimal or no impact on animal welfare. Nevertheless, the implementation rate of noninvasive genetic assessment in wildlife studies has been rather low. This might be caused by the perceived inefficiency of DNA material obtained non-invasively in comparison with DNA obtained from blood or tissues, or poorer performance in comparison with other approaches used in wildlife research. Therefore, the aim of this review was to evaluate the performance of noninvasive genetic assessment in comparison with other methods across different types of wildlife studies. Through a search of three scientific databases, 113 relevant studies were identified, published between the years 1997 and 2020. Overall, most of the studies (94%) reported equivalent or superior performance of noninvasive genetic assessment when compared with either invasive genetic sampling or another research method. It might be also cheaper and more time-efficient than other techniques. In conclusion, noninvasive genetic assessment is a highly effective research approach, whose efficacy and performance are likely to improve even further in the future with the development of optimized protocols.