In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds

The regenerative competence of inflorescence segments of Rauwolfia serpentina was assessed in M (Mitra et al., 1976) medium and its combinations with growth adjuncts such as cytokinins [N6-benzyladenine (6-BA - 0.5, 1.0 mg l-1), furfurylaminopurine (Kn-0.5, 1.0mg l-1) and auxin [α-naphthalene acetic acid (NAA - 0.5, 1.0 mg l-1)]. Inflorescence segments (1.00 cm in length) were used as explants. The regeneration response was obligatory to the use of growth regulators in the medium. Cytokinins favoured shoot bud mediated pathway of plantlet development and BAP favoured early shooting. The explants callused in NAA enriched medium. The callus was best maintained in NAA (0.5 mg l-1) through repeated sub-culturing. The entire plant of R. serpentina is the target of massive commercial collections resulting into shrinking habitats. Present study is the first ever report on the successful use of inflorescence segments for ex situ conservation of R. serpentina.

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Corrigendum to In Vitro Culture for Ex Situ Conservation of “Roveja” and Nutritional Considerations on This Italian Rare Legume

Natural Product Communications ◽

10.1177/1934578x20977238 ◽

2020 ◽

Vol 15 (11) ◽

pp. 1934578X2097723

Keyword(s):

In Vitro Culture ◽

Ex Situ Conservation ◽

Ex Situ

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In vitro propagation of endangered Dianthus taxa

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1511083m ◽

2015 ◽

pp. 83-98

Author(s):

Marija Markovic ◽

Mihailo Grbic ◽

Matilda Djukic

Keyword(s):

In Vitro Culture ◽

In Vitro Propagation ◽

Ex Situ Conservation ◽

Ex Situ ◽

Medium Term ◽

Research And Practice

The review of recent researches regarding the in vitro culture of 30 endangered Dianthus taxa is presented in this paper. Various in vitro protocols developed for selected rare and threatened Dianthus taxa are analysed in order to provide a useful synthesis of the data obtained with the main principles, techniques and recommendations for futher research and practice. The recapitulated data presented in this review can be used as a tool for the micropropagation of other endangered Dianthus taxa, enabling their propagation and obtaining a sufficient amount of plants for reintroduction. In addition, the obtained results represent the basis for ex situ conservation of the investigated taxa, especially for medium-term and long-term conservation (cryopreservation).

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Cryopreservation of Woody Crops: The Avocado Case

Plants ◽

10.3390/plants10050934 ◽

2021 ◽

Vol 10 (5) ◽

pp. 934

Author(s):

Chris O’Brien ◽

Jayeni Hiti-Bandaralage ◽

Raquel Folgado ◽

Alice Hayward ◽

Sean Lahmeyer ◽

...

Keyword(s):

Tissue Culture ◽

Ex Situ Conservation ◽

Plant Genetic Resources ◽

Ex Situ ◽

Conservation Strategies ◽

Recalcitrant Seed ◽

In Vitro Storage ◽

Germplasm Collections ◽

Seed Crops

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

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Ex situ conservation of genetic resources of field elm (Ulmus minor Mill) and European white elm (Ulmus laevis Pall)

Genetika ◽

10.2298/gensr0403221a ◽

2004 ◽

Vol 36 (3) ◽

pp. 221-227

Author(s):

Jelena Aleksic ◽

Sasa Orlovic

Keyword(s):

Genetic Resources ◽

Ex Situ Conservation ◽

Ex Situ ◽

In Vitro Production ◽

Ulmus Minor ◽

Conservation Of Genetic Resources ◽

Ulmus Laevis ◽

Vitro Production

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.

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Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Forests ◽

10.3390/f10111028 ◽

2019 ◽

Vol 10 (11) ◽

pp. 1028 ◽

Cited By ~ 1

Author(s):

Hazubska-Przybył

Keyword(s):

In Vitro Culture ◽

Protective Action ◽

Ex Situ ◽

Conservation Strategies ◽

Culture Techniques ◽

Effective Strategies ◽

Dry Climates ◽

Coniferous Tree Species ◽

Seed Propagation

The genus Juniperus (of the Cupressaceae family) is the second most prevalent group of conifers on Earth. Juniper species are widely dispersed in the Northern Hemisphere, in Europe and Asia, and in Africa and Central America. Juniper species are resistant to dry climates and can adapt to difficult environmental conditions. Most juniper species are important in both ecological and economic terms. However, today, many forests in which junipers occur are being reduced in size due to both natural causes (fires, for example) and human activity (uncontrolled exploitation of forests, etc.). Also, climate changes may have adversely affected the range of populations of different juniper species. For this reason, some juniper species are now categorized as rare or endangered, and require immediate protective action. Therefore, there is an urgent need to develop effective strategies for ex situ conservation, including reliable procedures for Juniperus sp. reproduction for future reintroduction and restoration programs. The conservation strategies used until now with traditional forestry techniques (seed propagation, rooted cuttings, grafting) have not been satisfactory in many cases. Thus, increasing attention is being paid to the possibilities offered by in vitro culture technology, which enables the conservation and mass clonal propagation of different coniferous tree species. In this mini-review, we summarize the current state of knowledge regarding the use of various methods of the propagation of selected Juniperus species, with a particular emphasis on in vitro culture techniques.

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