scholarly journals In vitro plant regeneration of Coccinea cordifolia (Linn.) Cogn., an anti-diabetic medicinal plant

2012 ◽  
Vol 47 (2) ◽  
pp. 187-190
Author(s):  
Chapol Kumar Roy ◽  
John Liton Munshia ◽  
Nadira Begum ◽  
Rahima Khatun ◽  
AKM Sayeed Hassanb
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Half Strength ◽  
In Vitro Plant ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro plant regeneration of a popularly used anti-diabetic medicinal plant, Coccinea cordifolia (Linn.) Cogn.(Cucurbitaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 88.2% of nodal explants responded to produce maximum number (6.2 ± 0.58) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. The survival rate of regenerated plantlets was 85%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11450   Bangladesh J. Sci. Ind. Res. 47(2), 187-190, 2012  

scholarly journals In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

2012 ◽  
Vol 47 (3) ◽  
pp. 297-302
Author(s):  
AKMS Hassan ◽  
F Haque ◽  
MAA Jahan ◽  
SK Roy
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Plumbago Zeylanica ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063 Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Ilse-Yazmín Arciniega-Carreón ◽  
Carmen Oliver-Salvador ◽  
María-Guadalupe Ramírez-Sotelo ◽  
Carlos Edmundo Salas
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Medicinal Plant ◽  
Metabolic Disorders ◽  
Naphthalene Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Shoot Production ◽  
In Vitro Plant ◽  
Indole 3 Acetic Acid

Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

scholarly journals In Vitro Shoot Proliferation and Plant Regeneration of Phyllanthus fraternus Webster (B. Bhuiamla), a Seasonal Medicinal Herb

1970 ◽  
Vol 46 (2) ◽  
pp. 205-210 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
R Khatun
Keyword(s):  
Plant Regeneration ◽  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Medicinal Herb ◽  
Nodal Explants ◽  
Efficient System ◽  
Half Strength ◽  
Phyllanthus Fraternus

An efficient system was developed for shoot proliferation and large scale plant regeneration of a seasonal multipotent medicinal herb, Phyllanthus fraternus Webster through in vitro culture. Shoot tips and nodal explants of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 88% of nodal explants responded to produce maximum number (16.8 ± 0.95) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%. Key words: Phyllanthus fraternus; Medicinal plant; Shoot proliferation; Regeneration; Acclimatization DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8187 Bangladesh J. Sci. Ind. Res. 46(2), 205-210, 2011

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