Comparative evaluation of recombinant LigB protein and heat-killed antigen-based latex agglutination test with microscopic agglutination test for diagnosis of bovine leptospirosis

2015 ◽  
Vol 47 (7) ◽  
pp. 1329-1335 ◽  
Author(s):  
Mohandoss Nagalingam ◽  
Sushma Rahim Assadi Thirumalesh ◽  
Triveni Kalleshamurthy ◽  
Nakkala Niharika ◽  
Vinayagamurthy Balamurugan ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Bovine Leptospirosis

scholarly journals Comparative evaluation of recombinant LigB based latex agglutination test with microscopic agglutination test for the diagnosis of wildlife leptospirosis

2020 ◽  
Vol 6 (3) ◽  
pp. 440-448
Author(s):  
Yosef Deneke ◽  
Rajib Deb ◽  
SM Lutful Kabir
Keyword(s):  
Comparative Evaluation ◽  
Acute Infection ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Iptg Induction ◽  
E Coli ◽  
Sera Samples

In the present study a comparative evaluation microscopic agglutination test with rLigB protein based latex agglutination test was carried out, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiralserovars. It was employed for serodiagnosis of leptospirosis. The 46 KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected wildlife field sera. A total of 80 wildlife sera samples were collec ted, including 27 wild feline sera samples (18 tigers, 8 lions, and 1 jaguar) obtained from Chhatbir zoo, Chandigarh, 42 sera samples (8 tigers, 4 lions and 6 leopards, 2 cheethals, 1 black buck, 12 buffaloes and 9 zoo staff) were received from Jodhpur zoo Rajasthan, 8 sera samples (4 tigers, 3 leopards, 1 lion) from Van Vihar National park, Bohpal, Madhya Pradesh and 3 sera samples (2 lions and 1 tiger) received from Biwani Mini zoo, Haryana, India. The result showed that sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity, under laboratory and field conditions, for the detection of Leptospirosis. Asian J. Med. Biol. Res. September 2020, 6(3): 440-448

Expression of Recombinant Leptospiral Surface Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Test

2020 ◽  
Vol 62 (11-12) ◽  
pp. 598-610
Author(s):  
Anusha Alamuri ◽  
K. Vinod Kumar ◽  
S. SowjanyaKumari ◽  
L. Linshamol ◽  
R. Sridevi ◽  
...  
Keyword(s):  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
E Coli ◽  
Bovine Leptospirosis

Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India

2014 ◽  
Vol 28 (4) ◽  
pp. 141-146 ◽  
Author(s):  
Yosef Deneke ◽  
T. Sabarinath ◽  
Neha Gogia ◽  
Jonathan Lalsiamthara ◽  
K.N. Viswas ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Bovine Leptospirosis

Detection of rotavirus in sewage and drinking water by Latex agglutination test

2019 ◽  
Vol 9 (2) ◽  
pp. p8694
Author(s):  
Muhammad Shoaib ◽  
Kokab Furqan ◽  
Sajjad ur Rahman ◽  
Ahsan Naveed ◽  
Amjad Islam Aqib ◽  
...  
Keyword(s):  
Drinking Water ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test
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