Expression of Recombinant Leptospiral Surface Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Test

2020 ◽  
Vol 62 (11-12) ◽  
pp. 598-610
Author(s):  
Anusha Alamuri ◽  
K. Vinod Kumar ◽  
S. SowjanyaKumari ◽  
L. Linshamol ◽  
R. Sridevi ◽  
...  
Keyword(s):  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
E Coli ◽  
Bovine Leptospirosis

Performance Comparison of a fliCh7 Real-Time PCR Assay with an H7 Latex Agglutination Test for Confirmation of the H Type of Escherichia coli O157:H7†

2009 ◽  
Vol 72 (10) ◽  
pp. 2195-2197 ◽  
Author(s):  
NEELAM NARANG ◽  
PINA M. FRATAMICO ◽  
GLENN TILLMAN ◽  
KITTY PUPEDIS ◽  
WILLIAM C. CRAY
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Escherichia Coli O157 ◽  
Pcr Assay ◽  
E Coli ◽  
Reference Strains ◽  
Pcr Test

Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. Positive identification of E. coli O157:H7 is made using biochemical tests and specific antisera or latex agglutination reagents for the O157 and H7 antigens. However, under certain conditions, some E. coli O157:H7 isolates can appear to be nonreactive with H7 antisera and may require multiple passages on motility medium to restore H7 antigenicity. In this study, we compared the performance of a real-time PCR test with that of a method using latex agglutination reagents to detect the presence of the fliCh7 gene or the H7 antigen, respectively, in E. coli O157:H7 isolates. One hundred twenty-six E. coli strains were tested including reference strains and strains isolated from meat. Lyophilized E. coli O157:H7 isolates were rehydrated and were plated on sheep blood agar without passage on motility medium. All strains were analyzed in parallel by a real-time PCR test targeting the fliCh7 gene and by a latex agglutination test that detects the H7 antigen. The real-time PCR assay showed 100% agreement with the H7 status reported for reference strains and E. coli O157:H7 meat isolates. The latex agglutination test results agreed with the H7 status reported for the E. coli O157:H7 reference strains and non-O157:H7 strains, except for one, E. coli O117:H7; however, 42% (42 of 100) of the E. coli O157:H7 meat isolates tested negative for the H7 antigen by latex agglutination. The real-time fliCh7 PCR test can be used to confirm E. coli O157:H7 strains that are not expressing the immunoreactive H7 antigen.

Comparative evaluation of recombinant LigB protein and heat-killed antigen-based latex agglutination test with microscopic agglutination test for diagnosis of bovine leptospirosis

2015 ◽  
Vol 47 (7) ◽  
pp. 1329-1335 ◽  
Author(s):  
Mohandoss Nagalingam ◽  
Sushma Rahim Assadi Thirumalesh ◽  
Triveni Kalleshamurthy ◽  
Nakkala Niharika ◽  
Vinayagamurthy Balamurugan ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Bovine Leptospirosis

scholarly journals Cloning and Expression of Leptospiral Immunoglobulin Like B Gene and Use of The Recombinant Antigen for The Diagnosis of Bovine and Caprine Leptospirosis

2020 ◽  
Vol 8 (2) ◽  
pp. 146-153
Author(s):  
Yosef Deneke ◽  
Rajib Deb
Keyword(s):  
Acute Infection ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Cloning And Expression ◽  
Indian Veterinary Research Institute ◽  
Iptg Induction ◽  
E Coli ◽  
Veterinary Research

In the present study recombinant LigB protein is employed in latex agglutination test, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiral serovars. It was employed  for serodiagnosis of leptospirosis. The 46KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected field sea. Western blot confirmed that rLigB is an immunodominant protein against which antibodies are produced during active infection. A total of 453 field sera, including 432 bovine sera, 18 caprine sera and three sera samples of buffalo bull collected post-mortem following death the animal from Indian Veterinary Research institute (IVRI) were tested using rLigB based LAT. The result showed that 300 sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, under laboratory and field conditions, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity for the detection of Leptospirosis. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 146-153

Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India

2014 ◽  
Vol 28 (4) ◽  
pp. 141-146 ◽  
Author(s):  
Yosef Deneke ◽  
T. Sabarinath ◽  
Neha Gogia ◽  
Jonathan Lalsiamthara ◽  
K.N. Viswas ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Bovine Leptospirosis

scholarly journals Comparative evaluation of recombinant LigB based latex agglutination test with microscopic agglutination test for the diagnosis of wildlife leptospirosis

2020 ◽  
Vol 6 (3) ◽  
pp. 440-448
Author(s):  
Yosef Deneke ◽  
Rajib Deb ◽  
SM Lutful Kabir
Keyword(s):  
Comparative Evaluation ◽  
Acute Infection ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Iptg Induction ◽  
E Coli ◽  
Sera Samples

In the present study a comparative evaluation microscopic agglutination test with rLigB protein based latex agglutination test was carried out, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiralserovars. It was employed for serodiagnosis of leptospirosis. The 46 KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected wildlife field sera. A total of 80 wildlife sera samples were collec ted, including 27 wild feline sera samples (18 tigers, 8 lions, and 1 jaguar) obtained from Chhatbir zoo, Chandigarh, 42 sera samples (8 tigers, 4 lions and 6 leopards, 2 cheethals, 1 black buck, 12 buffaloes and 9 zoo staff) were received from Jodhpur zoo Rajasthan, 8 sera samples (4 tigers, 3 leopards, 1 lion) from Van Vihar National park, Bohpal, Madhya Pradesh and 3 sera samples (2 lions and 1 tiger) received from Biwani Mini zoo, Haryana, India. The result showed that sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity, under laboratory and field conditions, for the detection of Leptospirosis. Asian J. Med. Biol. Res. September 2020, 6(3): 440-448

Detection of rotavirus in sewage and drinking water by Latex agglutination test

2019 ◽  
Vol 9 (2) ◽  
pp. p8694
Author(s):  
Muhammad Shoaib ◽  
Kokab Furqan ◽  
Sajjad ur Rahman ◽  
Ahsan Naveed ◽  
Amjad Islam Aqib ◽  
...  
Keyword(s):  
Drinking Water ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test
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