Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

2011 ◽  
Vol 35 (8) ◽  
pp. 521-530 ◽  
Author(s):  
Alida Maria Ferlazzo ◽  
Giuseppe Bruschetta ◽  
Patrizia Di Pietro ◽  
Pietro Medica ◽  
Anna Notti ◽  
...  
Keyword(s):  
Animal Species ◽  
31P Nmr ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes

scholarly journals Impact of membranetropic compounds on the sensitivity of mammalian erythrocytes to the posthypertonic shock action

2020 ◽  
pp. 31-38
Author(s):  
O Chabanenko ◽  
◽  
N Yershova ◽  
N Orlova ◽  
N Shpakova ◽  
...  
Keyword(s):  
Critical Micelle Concentration ◽  
Defect Formation ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes ◽  
Minimum Level ◽  
Rat Erythrocytes ◽  
Amphiphilic Compounds ◽  
Antihemolytic Activity ◽  
Mammalian Erythrocyte ◽  
Shock Action

The effect of cationic trifluoperazine (TFP) and nonionic decyl-β,D-glucopyranoside (DGP) on the sensitivity of human, rabbit and rat erythrocytes to the action of posthypertonic shock (PHS) at 0 °C was studied in this research. Trifluoperazine shows a high antihemolytic activity under conditions of PHS of human and animal erythrocytes at slight differences of values of effective concentrations. The value of antihemolytic activity of TFP for human and rabbit erythrocytes is ~ 60 %, and for rat cells the efficiency of this compound is approximately 1.4 times higher. The values of antihemolytic activity of DGP under PHS conditions of human and rat erythrocytes are comparable and amounts to 62 and 66 %, respectively. Significant differen­ces of this parameter (72 %) were found for rabbit cells compared with human erythrocytes. It was found that the size of plateau (the range of concentrations of amphiphilic compounds within the minimum level of erythrocyte hemolysis was observed) cationic TFP and nonionic DGP are significantly different. Thus, TFP has a narrow plateau (100–200 μmol/L), while DGP has a rather wide one (400–1600 μmol/L). In addition, a shift of the plateau concentrations of DGP to the region of higher values compared with TFP is observed, which is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP. Moreover, a shift of plateau concentrations of DGP to the region of higher values compared with TFP is observed, that is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP one. It was established that under PHS conditions of human erythrocyte, both compounds (TFP and DGP) show a commensurate antihemolytic activity. At the same time, for rabbit cells, DGP is more effective compared with TFP, and for rat erythrocytes, on the contrary, the efficiency of TFP is higher than DGP. This may be due to differences in the phospholipid composition of mammalian erythrocyte membranes. The results suggest that under PHS conditions the efficacy of membrane-tropic compounds is most likely due to their ability to incorporate into membrane to the defect formation areas, and thus significantly increase the critical hemolytic volume of cells, as a result, prevent their destruction.

Relationships between bile salts hydrophilicity and phospholipid composition in bile of various animal species

1986 ◽  
Vol 83 (3) ◽  
pp. 551-554 ◽  
Author(s):  
D. Alvaro ◽  
A. Cantafora ◽  
A.F. Attili ◽  
S. Ginanni Corradini ◽  
C. De Luca ◽  
...  
Keyword(s):  
Bile Salts ◽  
Animal Species ◽  
Phospholipid Composition

A study of the phospholipid composition of vegetables by using 31P-NMR

1992 ◽  
Vol 20 (2) ◽  
pp. 181S-181S
Author(s):  
M. ANTONIA MURCIA ◽  
JOSE VILLALAIN
Keyword(s):  
31P Nmr ◽  
Phospholipid Composition

scholarly journals Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

1995 ◽  
Vol 307 (2) ◽  
pp. 425-431 ◽  
Author(s):  
L M Lenton ◽  
C A Behm ◽  
F L Bygrave
Keyword(s):  
Mitochondrial Respiration ◽  
Post Infection ◽  
31P Nmr ◽  
Fasciola Hepatica ◽  
Phospholipid Composition ◽  
Liver Mitochondria ◽  
Esterified Fatty Acids ◽  
Phospholipid Species

The non-esterified fatty acid (NEFA) content and phospholipid composition of mitochondria isolated from the livers of Wistar rats infected with Fasciola hepatica were examined in relation to the aberrant mitochondrial respiration previously reported [Rule, Behm, and Bygrave (1989) Biochem. J. 260, 517-523]. At 2 weeks post-infection, elevated NEFA levels were associated with uncoupling of mitochondrial respiration that was reversible in vitro by the addition of BSA. State IV respiration rates showed a strong correlation with NEFA content. At 3 weeks post-infection, NEFA content had increased further and uncoupled mitochondria no longer showed any response to BSA. 31P-NMR analyses of cholate extracts of mitochondria from infected livers at 3 weeks post-infection revealed a marked loss of several major phospholipid species with a concomitant increase in catabolic products, particularly glycerophosphocholine and glycerophosphoethanolamine. Similar changes were observed in microsomal extracts. The NEFA content and phospholipid composition of mitochondria isolated from infected, athymic nude rats were not significantly different from uninfected, athymic rats. These findings suggest that uncoupling of liver mitochondria during infection with F. hepatica is the result of phospholipase activation mediated by the immune system of the host.

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