Phospholipid composition of dystrophic chicken erythrocyte plasmalemmae I. Isolation of a unique lipid in dystrophic erythrocyte membranes

1984 ◽  
Vol 778 (1) ◽  
pp. 112-120 ◽  
Author(s):  
Mark Kester ◽  
C.A. Privitera
Keyword(s):  
Phospholipid Composition ◽  
Erythrocyte Membranes ◽  
Chicken Erythrocyte ◽  
Dystrophic Chicken

scholarly journals Impact of membranetropic compounds on the sensitivity of mammalian erythrocytes to the posthypertonic shock action

2020 ◽  
pp. 31-38
Author(s):  
O Chabanenko ◽  
◽  
N Yershova ◽  
N Orlova ◽  
N Shpakova ◽  
...  
Keyword(s):  
Critical Micelle Concentration ◽  
Defect Formation ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes ◽  
Minimum Level ◽  
Rat Erythrocytes ◽  
Amphiphilic Compounds ◽  
Antihemolytic Activity ◽  
Mammalian Erythrocyte ◽  
Shock Action

The effect of cationic trifluoperazine (TFP) and nonionic decyl-β,D-glucopyranoside (DGP) on the sensitivity of human, rabbit and rat erythrocytes to the action of posthypertonic shock (PHS) at 0 °C was studied in this research. Trifluoperazine shows a high antihemolytic activity under conditions of PHS of human and animal erythrocytes at slight differences of values of effective concentrations. The value of antihemolytic activity of TFP for human and rabbit erythrocytes is ~ 60 %, and for rat cells the efficiency of this compound is approximately 1.4 times higher. The values of antihemolytic activity of DGP under PHS conditions of human and rat erythrocytes are comparable and amounts to 62 and 66 %, respectively. Significant differen­ces of this parameter (72 %) were found for rabbit cells compared with human erythrocytes. It was found that the size of plateau (the range of concentrations of amphiphilic compounds within the minimum level of erythrocyte hemolysis was observed) cationic TFP and nonionic DGP are significantly different. Thus, TFP has a narrow plateau (100–200 μmol/L), while DGP has a rather wide one (400–1600 μmol/L). In addition, a shift of the plateau concentrations of DGP to the region of higher values compared with TFP is observed, which is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP. Moreover, a shift of plateau concentrations of DGP to the region of higher values compared with TFP is observed, that is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP one. It was established that under PHS conditions of human erythrocyte, both compounds (TFP and DGP) show a commensurate antihemolytic activity. At the same time, for rabbit cells, DGP is more effective compared with TFP, and for rat erythrocytes, on the contrary, the efficiency of TFP is higher than DGP. This may be due to differences in the phospholipid composition of mammalian erythrocyte membranes. The results suggest that under PHS conditions the efficacy of membrane-tropic compounds is most likely due to their ability to incorporate into membrane to the defect formation areas, and thus significantly increase the critical hemolytic volume of cells, as a result, prevent their destruction.

Sphingomyelinase of chicken erythrocyte membranes

1975 ◽  
Vol 167 (1) ◽  
pp. 186-192 ◽  
Author(s):  
Daniella Hirshfeld ◽  
Abraham Loyter
Keyword(s):  
Erythrocyte Membranes ◽  
Chicken Erythrocyte

scholarly journals N-Acetyl-d-glucosamine specific hemagglutinin receptor ofVibrio choleraeO1 in chicken erythrocyte membranes

2002 ◽  
Vol 32 (3) ◽  
pp. 187-189
Author(s):  
D Sasmal ◽  
B Guhathakurta ◽  
S.K Bhattacharya ◽  
C.R Pal ◽  
A Datta
Keyword(s):  
Erythrocyte Membranes ◽  
Chicken Erythrocyte

Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

2011 ◽  
Vol 35 (8) ◽  
pp. 521-530 ◽  
Author(s):  
Alida Maria Ferlazzo ◽  
Giuseppe Bruschetta ◽  
Patrizia Di Pietro ◽  
Pietro Medica ◽  
Anna Notti ◽  
...  
Keyword(s):  
Animal Species ◽  
31P Nmr ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes

scholarly journals Modification of host cell membrane lipid composition by the intra-erythrocytic human malaria parasite Plasmodium falciparum

1991 ◽  
Vol 274 (1) ◽  
pp. 121-132 ◽  
Author(s):  
L L Hsiao ◽  
R J Howard ◽  
M Aikawa ◽  
T F Taraschi
Keyword(s):  
Fatty Acid ◽  
Malaria Parasite ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes ◽  
Fatty Acid Profiles ◽  
Human Malaria Parasite ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Erythrocyte Plasma Membrane ◽  
Fatty Acid Compositions

The phospholipid and fatty acid compositions of the host infected erythrocyte plasma membrane (IEPM) have been determined for erythrocytes infected with the human malaria parasite Plasmodium falciparum. IEPM were prepared by selective lysis of the host erythrocyte (but not of the parasite membranes) with 0.1% saponin, followed by differential centrifugation. The purity of the IEPM was determined by measuring the membrane-specific enzyme markers acetylcholinesterase, glutamate dehydrogenase and lactate dehydrogenase, and by immunoelectron microscopy using monoclonal antibodies specific for human erythrocyte glycophorin A (4E7) and for a 195 kDa parasite membrane glycoprotein (Pf6 3B10.1). Both approaches demonstrated that the host erythrocyte plasma membrane preparation was free from contamination by parasite membranes. During intra-erythrocytic development of the parasite, the phospholipid composition of the erythrocyte membrane was strikingly altered. IEPM contained more phosphatidylcholine (38.7% versus 31.7%) and phosphatidylinositol (2.1% versus 0.8%) and less sphingomyelin (14.6% versus 28.0%) than normal uninfected erythrocytes. Similar alterations in phospholipid composition were determined for erythrocyte membranes of parasitized cells isolated by an alternative method utilizing polycationic polyacrylamide microbeads (Affigel 731). The total fatty acid compositions of the major phospholipids in IEPM were determined by g.l.c. The percentage of polyunsaturated fatty acids in normal erythrocyte phospholipids (39.4%) was much higher than in phospholipids from purified parasites (23.3%) or IEPM (24.0%). The unsaturation index of phospholipids in IEPM was considerably lower than in uninfected erythrocytes (107.5 versus 161.0) and was very similar to that in purified parasites (107.5 versus 98.5). Large increases in palmitic acid (C16:0) (from 21.88% to 31.21%) and in oleic acid (C18:1) (from 14.64% to 24.60%), and major decreases in arachidonic acid (C20:4) (from 17.36% to 7.85%) and in docosahexaenoic acid (C22:6) (from 4.34% to 1.8%) occurred as a result of infection. The fatty acid profiles of individual phospholipid classes from IEPM resembled in many instances the fatty acid profiles of parasite phospholipids rather than those of uninfected erythrocytes. Analysis of IEPM from P. falciparum-infected erythrocytes (trophozoite stage) revealed that, during intra-erythrocytic maturation of the parasite, the host erythrocyte phospholipid composition was markedly refashioned. These alterations were not dependent on the method used to isolate the IEPM, with similar results obtained using either a saponin-lysis method or binding to Affigel beads. Since mature erythrocytes have negligible lipid synthesis and metabolism, these alterations must occur as a result of parasite-directed metabolism of erythrocyte lipids and/or trafficking of lipids between the parasite and erythrocyte membranes.

Studying the membrane structure of chicken erythrocytes by in situ atomic force microscopy

2014 ◽  
Vol 6 (20) ◽  
pp. 8115-8119 ◽  
Author(s):  
Yongmei Tian ◽  
Mingjun Cai ◽  
Haijiao Xu ◽  
Hongda Wang
Keyword(s):  
Atomic Force Microscopy ◽  
Membrane Structure ◽  
Erythrocyte Membranes ◽  
Chicken Erythrocyte ◽  
Force Microscopy ◽  
Outer Leaflet ◽  
Atomic Force ◽  
Chicken Erythrocytes

The smooth outer leaflet and protein-covered inner leaflet of chicken erythrocyte membranes are observed by atomic force microscopy under near-native conditions.

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