Molecular characterization of the interaction between the N-terminal region of Potato virus X (PVX) coat protein (CP) and Nicotiana benthamiana PVX CP-interacting protein, NbPCIP1

Virus Genes ◽  
2013 ◽  
Vol 46 (3) ◽  
pp. 517-523 ◽  
Author(s):  
Mi-Ri Park ◽  
Kook-Hyung Kim
Keyword(s):  
Coat Protein ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Terminal Region ◽  
Interacting Protein

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

Status of potato viruses in Tunisia and molecular characterization of Tunisian Potato Virus X (PVX) isolates

2017 ◽  
Vol 151 (3) ◽  
pp. 735-744 ◽  
Author(s):  
Ahmed Ben Hafsa ◽  
Nesrine Nabi ◽  
Besma M’rabet Saamali ◽  
Mohammed Salem Zellama ◽  
Sébastien Theil ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Viruses

scholarly journals Potato virus X movement in Nicotiana benthamiana: new details revealed by chimeric coat protein variants

2011 ◽  
Vol 13 (2) ◽  
pp. 198-203 ◽  
Author(s):  
CAMILLA BETTI ◽  
CHIARA LICO ◽  
DARIO MAFFI ◽  
SIMONE D'ANGELI ◽  
MARIA MADDALENA ALTAMURA ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Protein Variants

scholarly journals Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Juliane Röder ◽  
Christina Dickmeis ◽  
Rainer Fischer ◽  
Ulrich Commandeur
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Leaf Extracts ◽  
Systemic Spread ◽  
Mouth Disease Virus ◽  
Direct Fusion

Plant virus-based nanoparticles can be produced in plants on a large scale and are easily modified to introduce new functions, making them suitable for applications such as vaccination and drug delivery, tissue engineering, and in vivo imaging. The latter is often achieved using green fluorescent protein and its derivatives, but the monovalent fluorescent protein iLOV is smaller and more robust. Here, we fused the iLOV polypeptide to the N-terminus of the Potato virus X (PVX) coat protein, directly or via the Foot-and-mouth disease virus 2A sequence, for expression in Nicotiana benthamiana. Direct fusion of the iLOV polypeptide did not prevent the assembly or systemic spread of the virus and we verified the presence of fusion proteins and iLOV hybrid virus particles in leaf extracts. Compared to wild-type PVX virions, the PVX particles displaying the iLOV peptide showed an atypical, intertwined morphology. Our results confirm that a direct fusion of the iLOV fluorescent protein to filamentous PVX nanoparticles offers a promising tool for imaging applications.

scholarly journals Serological Identity of Potato Virus X (PVX) and PCR Characterization of Its Coat Protein (CP) Gene

2013 ◽  
Vol 2 (2) ◽  
pp. 92-96
Author(s):  
Qudsia Nosheen ◽  
Shahid Hameed ◽  
Sardar M. Mughal ◽  
Muhammad F. Abbas
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Crop ◽  
Potato Virus X ◽  
Datura Stramonium ◽  
Plant Viruses ◽  
Cp Gene ◽  
Antisense Primer ◽  
Specific Sense

Potato virus X (PVX) is among top ten most economically damaging plant viruses in the world and its increasing incidence is getting an alarming situation in potato crop of Pakistan. During two consecutive years (2010-11 and 2011-12), the incidence of PVX was recorded in potato fields at Rawalpindi, Islamabad, Faisalabad and Sahiwal. The samples were collected and subjected to Double Antibody Sandwiched (DAS) Enzyme Linked Immunosorbant Assay (ELISA) and average incidence of PVX was determined about 16.86% (OD405nm 1.38) during 2010-11 and 27.10% (OD405nm 0.479) in 2011-12. The infectivity of the virus was assayed through mechanical inoculation on Nicotiana tabacum cv. Samsun, N. rustica, Datura stramonium, Chenopodium sp. Gomphrena and Capsicum annuum producing local lesion, mosaic and mottling symptoms. Coat protein (CP) gene specific sense and antisense primer successfully amplified a 750bp fragments through Polymerase Chain Reaction (PCR) assay.

scholarly journals Characterization of Antigenic Epitopes of the Coat Protein of Potato Virus X by Systematic Immunochemical Analysis of Synthetic Peptides.

1993 ◽  
Vol 47 ◽  
pp. 994-998
Author(s):  
Matti Vuento ◽  
Maija Vihinen-Ranta ◽  
Katariina Paananen ◽  
Soile Blomqvist ◽  
Aarne Kurppa ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Synthetic Peptides ◽  
Potato Virus X ◽  
Immunochemical Analysis ◽  
Antigenic Epitopes
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