Optimization of Copper (II) Removal by Response Surface Methodology Using Root Nodule Endophytic Bacteria Isolated from Vigna unguiculata

2016 ◽  
Vol 227 (8) ◽  
Author(s):  
R. Manohari ◽  
K. N. Yogalakshmi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Vigna Unguiculata ◽  
Endophytic Bacteria ◽  
Root Nodule

RESPONSE SURFACE METHODOLOGY FOR STUDYING THE QUALITY CHARACTERISTICS OF COWPEA (VIGNA UNGUICULATA)-BASED TEMPEH

2009 ◽  
Author(s):  
GEORGE AMPONSAH ANNOR ◽  
ESTHER SAKYI-DAWSON ◽  
FIRIBU K. SAALIA ◽  
SAMUEL SEFA-DEDEH ◽  
EMMANUEL OHENE AFOAKWA ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Vigna Unguiculata ◽  
Quality Characteristics

scholarly journals Endophytic bacteria of Catharanthus roseus as an alternative source of Vindoline and application of response surface methodology to enhance its production

2019 ◽  
Vol 71 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Nawed Anjum ◽  
Ramesh Chandra
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Catharanthus Roseus ◽  
Endophytic Bacteria ◽  
Cost Effective ◽  
Ionization Mass ◽  
Nutritional Requirement ◽  
Alternative Source ◽  
Anticancer Compounds ◽  
Cost Efficient

The requirement for novel, cost-effective pharmaceutical compounds is growing. We screened endophytic bacteria of Catharanthus roseus for the production of vinca alkaloids. Vindoline-producing endophytic bacteria was identified as Microbacterium sp. Vindoline was purified and characterized using column chromatography, TLC, HPLC and electrospray ionization mass spectrometry. The subsequent aim of this study was to design a cost-efficient media that can maximize vindoline production from Microbacterium sp. Initial optimization of the nutritional requirement and of the process parameters were carried out by monothetic analysis. Key factors obtained were optimized by the central composite design of response surface methodology. This model was also validated by repeating the experiment under the optimized conditions, which resulted in maximum production of 82 ?g/L (predicted response was 76.21 ?g/L). Reliability of the experiments was confirmed by ANOVA analysis, regression coefficient, prediction vs actual chart and a 3D response plot, which determined the fit of the model. This study should serve as a reference point for the use of statistical techniques in bacterial systems for production of bioactive, anticancer compounds by plant endophytes.

Response surface methodology for optimisation of protein concentratepreparation from cowpea [Vigna unguiculata (L.) Walp]

2008 ◽  
Vol 110 (3) ◽  
pp. 735-741 ◽  
Author(s):  
Martin Alain Mune Mune ◽  
Samuel René Minka ◽  
Israël Lape Mbome
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Vigna Unguiculata
Sign in / Sign up

Export Citation Format

Share Document