Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

2007 ◽  
Vol 23 (11) ◽  
pp. 1585-1593 ◽  
Author(s):  
Sarvesh Kumar Soni ◽  
Jayant Malhar Khire
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Partial Characterization ◽  
Fermentation Conditions

PURIFICATION AND PARTIAL CHARACTERIZATION OF AN ENDO-POLYGALACTURONASE FROM ASPERGILLUS NIGER

2002 ◽  
Vol 26 (3) ◽  
pp. 253-265 ◽  
Author(s):  
CHUN-TENG GUO ◽  
WEI-MING XUE ◽  
TIAN-BAO CHEN ◽  
DENG WEN-HAN ◽  
PING-FAN RAO
Keyword(s):  
Aspergillus Niger ◽  
Partial Characterization

Multiple forms of glycosidases in an enzyme preparation from Aspergillus niger: Partial characterization of a β-apiosidase

1997 ◽  
Vol 21 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Ziya Günata ◽  
Isabelle Dugelay ◽  
M.J. Vallier ◽  
J.C. Sapis ◽  
C. Bayonove
Keyword(s):  
Aspergillus Niger ◽  
Enzyme Preparation ◽  
Partial Characterization ◽  
Multiple Forms

Purification and Partial Characterization of Cellulase Produced by Aspergillus niger Cultured on Vitellaria paradoxa shells

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Abdulhakeem Olarewaju Sulyman ◽  
Yusuf A Iyanda ◽  
Afolabi Olaniyi Opasola ◽  
OtunOla Adedayo ◽  
Raliat Abimbola Aladodo
Keyword(s):  
Aspergillus Niger ◽  
Specific Activity ◽  
Gel Filtration ◽  
Inoculum Size ◽  
Partial Characterization ◽  
Effect Of Temperature ◽  
Vitellaria Paradoxa ◽  
Endoglucanase Activity ◽  
Ammonium Sulphate Precipitation

This research investigated the purification and partial characterization of cellulase produced by Aspergillus niger cultured on Vitellaria paradoxa shells. Cellulase (endoglucanase) from A. niger was produced under optimum fermentation conditions at 35 °C, pH 4.7, V. paradoxa, 4 g/L, inoculum size of 10 mm and the fermentation media incubated for 120 hours. The crude endoglucanase obtained were partially purified by subjecting to ammonium sulphate precipitation, dialysis and gel filtration chromatography for further purification. The effect of temperature and pH on the activity of purified endoglucanase was determined. Cellulase was purified to 734.33 folds by Sephadex G-100 column chromatography with a specific activity and yield of 4.406 U/mg and 63.03% respectively. Fractions 4 and 7 contained the highest endoglucanase activity out of 18 fractions collected and the two fractions were pooled for further analysis. The activity of purified endoglucanase was optimum at a temperature of 40 °C and pH 5. Therefore, the purified endoglucanase produced may be explored in detergent industry.

Production and partial characterization of glucose oxidase and catalase from xerophytic strain of Aspergillus niger

2014 ◽  
Vol 8 (29) ◽  
pp. 2788-2800
Author(s):  
Vernica Garay Flores Roco ◽  
Patricia Segura Ceniceros Elda ◽  
de Len Gmez Rosalba ◽  
Balvantn Garca Cecilia ◽  
Luis Martnez Hernndez Jos ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Partial Characterization

scholarly journals Production and Partial Characterization of an Extracellular Phytase Produced by <i>Muscodor</i> sp. under Submerged Fermentation

2016 ◽  
Vol 06 (01) ◽  
pp. 23-32 ◽  
Author(s):  
Natálie Martins Alves ◽  
Luis Henrique Souza Guimarães ◽  
Roberta Hilsdorf Piccoli ◽  
Patrícia Gomes Cardoso
Keyword(s):  
Submerged Fermentation ◽  
Partial Characterization ◽  
Extracellular Phytase

Partial characterization of esterases from Fusarium culmorum grown in media containing di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation

2018 ◽  
Vol 44 ◽  
pp. S137 ◽  
Author(s):  
E. Ocaña-Romo ◽  
L. Ferrer-Parra ◽  
D.I. López-Nicolás ◽  
R. Martínez-Castillo ◽  
J.P. Montiel-Cina ◽  
...  
Keyword(s):  
Solid State ◽  
Submerged Fermentation ◽  
Fusarium Culmorum ◽  
Partial Characterization ◽  
Ethyl Hexyl

scholarly journals SCREENING OF ASPERGILLUS NIGER ISOLATED FROM SOIL FOR PECTINASE PRODUCTION

2020 ◽  
Vol 4 (2) ◽  
pp. 244-249
Author(s):  
Abubakar Madika ◽  
Ulem Asagha Eugene ◽  
Musa Bishir ◽  
Mamunu Abdulkadir Sulaiman ◽  
Ibrahim Mohammed Hussaini
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Botanical Garden ◽  
Culture Technique ◽  
Soil Samples ◽  
Citrus Pectin ◽  
Fermentation Conditions ◽  
Cotton Blue ◽  
Pectinase Activity ◽  
Pectinase Production

This study was conducted to screen for pectinase production by Aspergillus niger isolated from soil samples collected from three different locations within Ahmadu Bello University, Zaria, namely; botanical garden, refuse dump and sheep pen sites. A total of fifteen (15) soil samples were collected from different locations and used for isolation by a cultural method. Isolates suspected to be Aspergillus niger were further identified by microscopic examination of the lactophenol cotton blue stained-preparation and slide culture technique. The isolates were then screened in a pectin-containing medium for their pectinase activity. The isolates were further subjected to pectinase production using citrus pectin as the substrate under submerged fermentation conditions. Seven (7) isolates were confirmed to be Aspergillus niger with percentage occurrence of 60% each from sheep pen (SP) and refuse dumpsites (RD), and 20% from the botanical garden (BG). Aspergillus niger RD3 produced the highest zone of pectin hydrolysis (53 ± 1.1 mm) while isolate RD5 produced the lowest (35 ± 3.1 mm). Under submerged fermentation conditions, Aspergillus niger SP5 had the highest pectinase activity of 2.92 U/mL while isolate RD4 had the lowest pectinase activity of 1.29 U/mL. Aspergillus niger can be readily isolated from various soil types with the highest frequency in soils from sheep pen and refuse dumpsites. All the Aspergillus niger isolates demonstrated the potential for pectinase production. The study reveals the potential of various Aspergillus niger isolates from different soil in the production of pectinase.

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