scholarly journals SCREENING OF ASPERGILLUS NIGER ISOLATED FROM SOIL FOR PECTINASE PRODUCTION

2020 ◽  
Vol 4 (2) ◽  
pp. 244-249
Author(s):  
Abubakar Madika ◽  
Ulem Asagha Eugene ◽  
Musa Bishir ◽  
Mamunu Abdulkadir Sulaiman ◽  
Ibrahim Mohammed Hussaini
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Botanical Garden ◽  
Culture Technique ◽  
Soil Samples ◽  
Citrus Pectin ◽  
Fermentation Conditions ◽  
Cotton Blue ◽  
Pectinase Activity ◽  
Pectinase Production

This study was conducted to screen for pectinase production by Aspergillus niger isolated from soil samples collected from three different locations within Ahmadu Bello University, Zaria, namely; botanical garden, refuse dump and sheep pen sites. A total of fifteen (15) soil samples were collected from different locations and used for isolation by a cultural method. Isolates suspected to be Aspergillus niger were further identified by microscopic examination of the lactophenol cotton blue stained-preparation and slide culture technique. The isolates were then screened in a pectin-containing medium for their pectinase activity. The isolates were further subjected to pectinase production using citrus pectin as the substrate under submerged fermentation conditions. Seven (7) isolates were confirmed to be Aspergillus niger with percentage occurrence of 60% each from sheep pen (SP) and refuse dumpsites (RD), and 20% from the botanical garden (BG). Aspergillus niger RD3 produced the highest zone of pectin hydrolysis (53 ± 1.1 mm) while isolate RD5 produced the lowest (35 ± 3.1 mm). Under submerged fermentation conditions, Aspergillus niger SP5 had the highest pectinase activity of 2.92 U/mL while isolate RD4 had the lowest pectinase activity of 1.29 U/mL. Aspergillus niger can be readily isolated from various soil types with the highest frequency in soils from sheep pen and refuse dumpsites. All the Aspergillus niger isolates demonstrated the potential for pectinase production. The study reveals the potential of various Aspergillus niger isolates from different soil in the production of pectinase.

scholarly journals Pectinase production by Aspergillus niger isolated from decomposed apple skin

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Point Of View ◽  
Media Composition ◽  
Maximal Level ◽  
Pectinolytic Activity ◽  
Pectinase Activity ◽  
Pectinase Production

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013

Synthesis of Pectin Methylesterase from Aspergillus niger in Submerged Fermentation Using as Citrus Pectin and Orange Peel as Inducers

2018 ◽  
Vol 14 (4) ◽  
pp. 212-221
Author(s):  
Jonaina Pili ◽  
Cindy Elena Bustamante Vargas ◽  
Carolina Elisa Demaman Oro ◽  
Geciane Toniazzo Backes ◽  
Eunice Valduga ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Orange Peel ◽  
Pectin Methylesterase ◽  
Citrus Pectin

scholarly journals Study of pectinase production in submerged fermentation using different strains of Aspergillus Niger

2009 ◽  
Vol 1 (2) ◽  
pp. 13-17 ◽  
Author(s):  
Yogesh Khairnar ◽  
◽  
Joshi B ◽  
Adarsh Mujapara ◽  
Amol Jhadav ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Pectinase Production ◽  
Different Strains

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

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