Phytase production by fermentation of recombinant Pichia pastoris in monosodium glutamate wastewater

2009 ◽  
Vol 25 (9) ◽  
pp. 1643-1649 ◽  
Author(s):  
Yingguo Bai ◽  
Peilong Yang ◽  
Yaru Wang ◽  
Pengjun Shi ◽  
Huiying Luo ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Monosodium Glutamate ◽  
Phytase Production ◽  
Recombinant Pichia Pastoris

Medium Optimization for the Production of Phytase by Recombinant Pichia pastoris Grown on Glycerol

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Min Liu ◽  
Gabriel Potvin ◽  
Yiru Gan ◽  
Zhanbin Huang ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Phytase Production ◽  
Medium Components ◽  
Gap Promoter ◽  
Recombinant Pichia Pastoris ◽  
Burman Design ◽  
Statistical Designs ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Three Components

Based on statistical designs, minimal salts medium, commonly used for yeast cultivation, was optimized to maximize GAP promoter-mediated phytase production by recombinant Pichia pastoris grown on glycerol. A Plackett-Burman design was followed to screen medium components to determine those that significantly affected phytase production. Of the 8 components studied, the concentrations of K2SO4, CaSO4•2H2O and MgSO4•7H2O were identified as having a significant effect. These three components were subsequently optimized by response surface methodology using a central composite design. The optimal concentrations of the three components, leading to a maximal extracellular phytase activity of 161.64 U/ml, were K2SO4 13.25g/l, CaSO4•2H2O 1.03g/l and MgSO4•7H2O 17.94g/l. The activity measured in cultures using optimized growth medium is significantly higher than the 73.31 U/ml measured in cultures using standard minimal salts media. The theoretical phytase yields predicted by the developed model were very close to experimentally obtained values.

scholarly journals Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris

2018 ◽  
Vol 212 ◽  
pp. 80-87 ◽  
Author(s):  
Martine Moulin ◽  
Gernot A. Strohmeier ◽  
Melanie Hirz ◽  
Katherine C. Thompson ◽  
Adrian R. Rennie ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Neutron Scattering ◽  
Recombinant Pichia Pastoris

Constitutive expression of alkaline β-mannanase in recombinant Pichia pastoris

2014 ◽  
Vol 49 (12) ◽  
pp. 2025-2029 ◽  
Author(s):  
Taicheng Zhu ◽  
Hongbing Sun ◽  
Pengfei Li ◽  
Yanfen Xue ◽  
Yin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Constitutive Expression ◽  
Recombinant Pichia Pastoris

Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris

2013 ◽  
Vol 40 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Taicheng Zhu ◽  
Haifeng Hang ◽  
Ju Chu ◽  
Yingping Zhuang ◽  
Siliang Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Mixed Feeding ◽  
Recombinant Pichia Pastoris ◽  
Cellular Stresses
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