Development of leucine-rich repeat polymorphism, amplified fragment length polymorphism, and sequence characterized amplified region markers to the Cronartium ribicola resistance gene Cr2 in western white pine (Pinus monticola)

2008 ◽  
Vol 4 (4) ◽  
pp. 601-610 ◽  
Author(s):  
Jun-Jun Liu ◽  
Abul K. M. Ekramoddoullah
Keyword(s):  
Resistance Gene ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Pinus Monticola ◽  
Leucine Rich Repeat ◽  
Repeat Polymorphism ◽  
White Pine ◽  
Sequence Characterized Amplified Region ◽  
Western White Pine

An anchored AFLP- and retrotransposon-based map of diploid Avena

Genome ◽  
2000 ◽  
Vol 43 (5) ◽  
pp. 736-749 ◽  
Author(s):  
Gong-Xin Yu ◽  
Roger P Wise
Keyword(s):  
Resistance Gene ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Rust Resistance ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Crown Rust ◽  
Crown Rust Resistance ◽  
Resistance Specificity ◽  
Specific Amplification

A saturated genetic map of diploid oat was constructed based on a recombinant inbred (RI) population developed from a cross between Avena strigosa (Cereal Introduction, C.I. 3815) and A. wiestii (C.I. 1994). This 513-locus map includes 372 AFLP (amplified fragment length polymorphism) and 78 S-SAP (sequence-specific-amplification polymorphism) markers, 6 crown-rust resistance loci, 8 resistance-gene analogs (RGAs), one morphological marker, one RAPD (random amplified polymorphic DNA) marker, and is anchored by 45 grass-genome RFLP (restriction fragment length polymorphism) markers. This new A. strigosa × A. wiestii RI map is colinear with a diploid Avena map from an A. atlantica × A. hirtula F2 population. However, some linkage blocks were rearranged as compared to the RFLP map derived from the progenitor A. strigosa × A. wiestii F2 population. Mapping of Bare-1-like sequences via sequence-specific AFLP indicated that related retrotransposons had considerable heterogeneity and widespread distribution in the diploid Avena genome. Novel amplified fragments detected in the RI population suggested that some of these retrotransposon-like sequences are active in diploid Avena. Three markers closely linked to the Pca crown-rust resistance cluster were identified via AFLP-based bulk-segregant analysis. The derived STS (sequence-tagged-site) marker, Agx4, cosegregates with Pc85, the gene that provides resistance specificity to crown-rust isolate 202 at the end of the cluster. This framework map will be useful in gene cloning, genetic mapping of qualitative genes, and positioning QTL (quantitative trait loci) of agricultural importance.Key words: AFLP, Bare-1 retrotransposon, sequence-specific-amplification polymorphism (S-SAP), resistance-gene analog, crown-rust resistance, Pca, Gramineae, grass anchor probe.

scholarly journals Use of Amplified Fragment Length Polymorphism and Sequence Characterized Amplified Region Marker for Identifying the Sex of the Oxyeleotris marmorata

2021 ◽  
Vol 44 (1) ◽  
Author(s):  
Douglas Law ◽  
Voon Chun Ping ◽  
Tan Chin Yee ◽  
Herryawan Ryadi Eziwar Dyari ◽  
Maizan Haiji Mohamed ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Scar Marker ◽  
Amplified Fragment Length Polymorphism ◽  
Breeding Programs ◽  
Sequence Characterized Amplified Region ◽  
Sex Marker ◽  
Daunting Task ◽  
Sexual Identification

There is a huge demand for the Oxyeleotris marmorata, especially in Asian markets. However, farmers are unable to provide a constant supply of this fish to meet the demand, which is estimated to be around 100 metric tonnes per annum. One of the reasons that are hindering the supply is the low success rate of O. marmorata breeding programs. These breeding programs rely on many factors for their success, one of which is the use of genuine male and female adults, although determining these could be a daunting task. This research was carried out in an attempt to determine a sex marker for the O. marmorata using the amplified fragment length polymorphism (AFLP) method. Of the 30×30 AFLP primer mixtures screened, the E-TAA and M-CTT primer pair had an amplified ~600 bp marker that was specific to the female. This ~600 bp AFLP marker was later used to design a 464 bp sequence characterized amplified region (SCAR) marker. Thus, it has been suggested that the SCAR marker obtained has the potential to be used for the sexual identification of the O. marmorata at the juvenile stage, thereby enabling them to be used in breeding programs.

scholarly journals A Novel Rust Resistance Gene in Maize Showing Overdominance

1998 ◽  
Vol 11 (3) ◽  
pp. 242-245 ◽  
Author(s):  
Donna E. Delaney ◽  
Craig A. Webb ◽  
Scot H. Hulbert
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Resistance Gene ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Rust Resistance ◽  
Fragment Length Polymorphism ◽  
Resistance Locus ◽  
Chromosome 6 ◽  
Restriction Fragment Length

The Rp8 rust resistance locus of maize is unique in that only Rp8-A/Rp8-B heterozygotes provide resistance and homozygotes are susceptible. A third allele, Rp8-C, provides no resistance in homozygotes or heterozygous combinations. Rp8 maps to the long arm of chromosome 6, near the restriction fragment length polymorphism marker umc21.

Linkage map of Cucumis melo including phenotypic traits and sequence-characterized genes

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 761-773 ◽  
Author(s):  
Leah Silberstein ◽  
Irina Kovalski ◽  
Yariv Brotman ◽  
Christophe Perin ◽  
Catherine Dogimont ◽  
...  
Keyword(s):  
Linkage Map ◽  
Resistance Gene ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeats ◽  
Cucumis Melo ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Phenotypic Traits ◽  
Simple Sequence

A new linkage map of Cucumis melo, derived from the F2 progeny of a cross between PI 414723 and C. melo 'TopMark' is presented. The map spans a total of 1421 cM and includes 179 points consisting of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSRs), simple sequence repeats (SSRs), and restriction fragment length polymorphism (RFLP) markers. The map also includes an aphid resistance trait (Vat) and the sex type gene, andromonoecious (a), the two of which are important in resistance breeding and the control of hybrid seed production, as well as a seed-color gene, Wt-2. Most RFLPs represent sequence-characterized cDNA probes from C. melo and Cucumis sativus. These include resistance gene homologues and genes involved in various aspects of plant development and metabolism. A sub-set of our SSR and RFLP markers were also mapped, as part of this study, on additional mapping populations that were published for this species. This provides important reference points ("anchors"), enabling us to identify several linkage groups with respect to other melon maps.Key words: Cucumis melo, melon, genetic map, molecular markers, resistance gene homologues.

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