Biophysical Settings that Influenced Plantation Survival During the 2015 Wildfires in Northern Rocky Mountain Moist Mixed-Conifer Forests

Fire suppression and the loss of western white pine (WWP) have made northern Rocky Mountain moist mixed-conifer forests less disturbance resilient. Although managers are installing hundreds of plantations, most of these plantations have not experienced wildfire since establishment. In 2015, wildfires burned through one hundred WWP plantations in this region, providing an opportunity to evaluate the effects of wildfires on sapling survival. A Weibull distribution approach was used to characterize the variation of fire severity pixels, as indicated by the differenced normalized burn ratio. The distribution parameters provided a method to identify the biophysical setting and plantation characteristics influencing fire severity and sapling survival. Plantations located on lower slope positions were more resistant to wildfires than plantations located midslope or close to the ridges. Snow water equivalent was positively correlated with wildfire resistance and resilience. Results will help focus reforestation efforts and identify locations where future plantations can potentially survive wildfires. Study Implications This study examined wildfire effects on western white pine plantations, with the intention to inform managers where to locate plantations that will be more resistant to wildfires and determine which plantations may require postfire reforestation. Plantations were more resilient and resistant to wildfires when they occurred on lower slopes, even when steep, indicating these places may be better suited for future plantations. Plantations located on upper slopes and ridges are vulnerable to wildfire even when located on moist habitat types and will likely need reforestation.

Multidecadal Growth of Western White Pine and Interior Douglas-Fir Following Site Preparation

Site preparation is used to favor seedling regeneration and establishment by enhancing growing conditions and increasing resource availability, yet few studies have compared different site preparation techniques on growth and yield of trees over multiple decades. We destructively sampled 34-year old trees of western white pine (Pinus monticola Douglas ex D. Don) and Interior Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. glauca (Beissn.) Franco planted at two sites using a replicated experiment to test the effectiveness of different site preparation treatments: (1) no site preparation, (2) scalping, (3) bedding, and (4) bedding plus three years of competition control with herbicide. Growth and yield were compared among the treatments, and models of growth were developed for each species and treatment combination. The herbicide treatment was the only treatment that consistently improved growth and yield of both species resulting in 19%–30% gains in height, 43%–63% gains in diameter, and 31%–109% gains in stem volume by age 34. Height growth response to herbicide was sustained until age 14 for white pine and age 12 for Douglas-fir, while the diameter response was sustained until age 23 for white pine and 20 for Douglas-fir. The later peak in growth for white pine suggests a better response to treatment and that the species was able to maintain higher growth following crown closure. Both species exhibited a Type 2 growth response to herbicide, suggesting competition control resulted in sustained gains over time with associated age shifts of 8.5 and 9.7 years for white pine and 7.1 and 10.2 years for Douglas-fir, height and diameter, respectively. This compares to scalping and bedding which produced no detectable difference in growth compared to the control, and in some instances, reduced growth. In the Northern Rocky Mountains, moisture is most limiting. This is likely why trees showed the greatest response to competition control. Interestingly, this growth was sustained well beyond seedling establishment.

Characterization of the western white pine TIR-NBS-LRR (PmTNL2) gene by transcript profiling and promoter analysis

Proteins with nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) have been reported to play important roles in plant disease resistance, growth, and development. However, no comprehensive analysis of this protein family has been performed in conifers. Here we report that the Pinus monticola PmTNL2 gene is a member of the NBS-LRR superfamily. Quantitative reverse transcription-PCR (qRT-PCR) analysis revealed that the PmTNL2 transcript was expressed in a tissue-specific pattern with extensive regulation by various environmental stimuli in western white pine seedlings, suggesting its wide involvement in stress defense and diverse developmental processes. In silico analysis of the PmTNL2 promoter region revealed multiple cis-regulatory elements characterized with potential functions for development-, light-, and stress-regulated transcript expression. Expression patterns were largely confirmed by PmTNL2 promoter-directed reporter gene expression using stable transgenic Arabidopsis plants. Notably, the PmTNL2 promoter activity was highly expressed in shoot apical and floral meristems and was induced strongly with vascular specificity by pathogen infection. Our data has provided a fundamental insight into both expression regulation and putative functions of the PmTNL2 gene in the context of plant growth and development, as well as in responses to environmental stressors. Promoter application as a potential tool for tree improvement was further discussed.

Armillaria altimontana Is Associated with Healthy Western White Pine (Pinus monticola): Potential in Situ Biological Control of the Armillaria Root Disease Pathogen, A. solidipes

Research Highlights: Two genets of Armillaria altimontana Brazee, B. Ortiz, Banik, and D.L. Lindner and five genets of Armillaria solidipes Peck (as A. ostoyae [Romagnesi] Herink) were identified and spatially mapped within a 16-year-old western white pine (Pinus monticola Doug.) plantation, which demonstrated distinct spatial distribution and interspecific associations. Background and Objectives: A. solidipes and A. altimontana frequently co-occur within inland western regions of the contiguous USA. While A. solidipes is well-known as a virulent primary pathogen that causes root disease on diverse conifers, little has been documented on the impact of A. altimontana or its interaction with A. solidipes on growth, survival, and the Armillaria root disease of conifers. Materials and Methods: In 1971, a provenance planting of P. monticola spanning 0.8 ha was established at the Priest River Experimental Forest in northern Idaho, USA. In 1987, 2076 living or recently dead trees were measured and surveyed for Armillaria spp. to describe the demography and to assess the potential influences of Armillaria spp. on growth, survival, and the Armillaria root disease among the study trees. Results: Among the study trees, 54.9% were associated with Armillaria spp. The genets of A. altimontana and A. solidipes comprised 82.7% and 17.3% of the sampled isolates (n = 1221) from the study plot, respectively. The mapped distributions showed a wide, often noncontiguous, spatial span of individual Armillaria genets. Furthermore, A. solidipes was found to be uncommon in areas dominated by A. altimontana. The trees colonized by A. solidipes were associated with a lower tree growth/survival and a substantially higher incidence of root disease than trees colonized only by A. altimontana or trees with no colonization by Armillaria spp. Conclusions: The results demonstrate that A. altimontana was not harmful to P. monticola within the northern Idaho planting. In addition, the on-site, species-distribution patterns suggest that A. altimontana acts as a long-term, in situ biological control of A. solidipes. The interactions between these two Armillaria species appear critical to understanding the Armillaria root disease in this region.

Effects of Grosmannia clavigera and Leptographium longiclavatum on Western White Pine Seedlings and the Fungicidal Activity of Alamo®, Arbotect®, and TREE-äge®

Bark beetles carry a number of associated organisms that are transferred to the host tree upon attack that are thought to play a role in tree decline. To assess the pathogenicity to western white pine (WWP; Pinus monticola) of fungi carried by the mountain pine beetle (MPB; Dendroctonus ponderosae), and to evaluate the potential for systemic prophylactic treatments for reducing fungal impacts, experiments were conducted with WWP seedlings to meet three objectives: 1) evaluate pathogenicity of two MPB-associated blue-stain fungi; 2) evaluate phytotoxicity of tree injection products; 3) evaluate the anti-fungal activity of tree injection products, in vitro and in vivo, toward the associated blue-staining fungi. To evaluate pathogenicity, seedlings were inoculated with Grosmannia clavigera or Leptographium longiclavatum, common fungal associates of MPB. Seedling mortality at four months after inoculation was 50% with L. longiclavatum and 90% with G. clavigera, both significantly higher than controls and thereby demonstrating pathogenicity. Phytotoxic effects of TREE-äge®, Alamo®, and Arbotect® were evaluated by stem injection; no phytotoxic effects were observed. Anti-fungal properties of the same three products were evaluated in vitro against G. clavigera, where Alamo was most active. Co-inoculation of G. clavigera and L. longiclavatum into seedlings after a stem injection of Alamo showed significantly less mortality and lesion formation than either species alone. Results support the hypothesis that MPB blue-stain associates, particularly G. clavigera, promote death of WWP when attacked by MPB. These findings suggest that the administration of a fungicide with insecticide for tree protection against bark beetles may be advantageous.