TcMYB1, TcMYB4, and TcMYB8 participate in the regulation of lignan biosynthesis in Taiwania cryptomerioides Hayata

2019 ◽  
Vol 15 (5) ◽  
Author(s):  
Nien-Ting Chiang ◽  
Chi-Hsiang Wen ◽  
Fang-Hua Chu
Keyword(s):  
Lignan Biosynthesis ◽  
Taiwania Cryptomerioides

The gene expression and enzymatic activity of pinoresinol-lariciresinol reductase during wood formation in Taiwania cryptomerioides Hayata

Holzforschung ◽  
2019 ◽  
Vol 73 (2) ◽  
pp. 197-208 ◽  
Author(s):  
Nien-Ting Chiang ◽  
Li-Ting Ma ◽  
Yi-Ru Lee ◽  
Nai-Wen Tsao ◽  
Chih-Kai Yang ◽  
...  
Keyword(s):  
Wood Formation ◽  
Rna Seq ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reaction Products ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Lignan Biosynthesis ◽  
Polymerase Chain ◽  
Taiwania Cryptomerioides ◽  
Pinoresinol Lariciresinol Reductase

AbstractTaiwania (Taiwania cryptomerioidesHayata) is an indigenous conifer species of Taiwan. Various secondary metabolites of Taiwania with diverse bioactivities have been identified, and lignans are especially abundant in the heartwood (hW). In the present study, the wood of this species was separated to cambium (Cam), sapwood (sW), transition zone (TZ) and hW and their transcriptomes were sequenced. Three pinoresinol-lariciresinol reductases (PLRs; designatedTcPLR1,TcPLR2.2andTcPLR3), which are responsible for lignan biosynthesis, were cloned and their expressions in wood tissues were detected.TcPLRs had higher expression levels in Cam and sW in RNA-seq and reverse transcriptase-polymerase chain reaction (RT-PCR) results. Liquid chromatography-mass spectrometry (LC-MS) analysis of the reaction products of TcPLRs revealed that TcPLR1 can reduce (+)-pinoresinol to lariciresinol, and both TcPLR2.2 and TcPLR3 could reduce (+)-pinoresinol to lariciresinol and secoisolariciresinol.

Development of polymorphic microsatellite markers for the Tertiary relict tree species Taiwania cryptomerioides (Cupressaceae) in East Asia

2021 ◽  
Vol 48 (3) ◽  
pp. 3031-3036
Author(s):  
Mengyun Qin ◽  
Ningning Zhang ◽  
Shixin Zhu ◽  
Caipeng Yue ◽  
Jinyong Huang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
East Asia ◽  
Tree Species ◽  
Polymorphic Microsatellite ◽  
Taiwania Cryptomerioides

scholarly journals Unveiling Monoterpene Biosynthesis in Taiwania cryptomerioides via Functional Characterization

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2404
Author(s):  
Li-Ting Ma ◽  
Pi-Ling Liu ◽  
Yang-Tui Cheng ◽  
Tz-Fan Shiu ◽  
Fang-Hua Chu
Keyword(s):  
Phylogenetic Analysis ◽  
Functional Characterization ◽  
In Vitro Assay ◽  
Conifer Species ◽  
Vitro Assay ◽  
Catalytic Residues ◽  
Monoterpene Synthase ◽  
Monoterpene Biosynthesis ◽  
Taiwania Cryptomerioides

Taiwania cryptomerioides is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in T. cryptomerioides, this study used transcriptome mining to identify candidates with tentative monoterpene synthase activity. Along with the phylogenetic analysis and in vitro assay, two geraniol synthases (TcTPS13 and TcTPS14), a linalool synthase (TcTPS15), and a β-pinene synthase (TcTPS16), were functionally characterized. Via the comparison of catalytic residues, the Cys/Ser at region 1 might be crucial in determining the formation of α-pinene or β-pinene. In addition, the Cupressaceae monoterpene synthases were phylogenetically clustered together; they are unique and different from those of published conifer species. In summary, this study aimed to uncover the ambiguous monoterpenoid network in T. cryptomerioide, which would expand the landscape of monoterpene biosynthesis in Cupressaceae species.

Diversity in lignan biosynthesis

2003 ◽  
Vol 2 (3) ◽  
pp. 371-390 ◽  
Author(s):  
Toshiaki Umezawa
Keyword(s):  
Lignan Biosynthesis

Taiwanoids A–D, four dimeric diterpenoids featuring tetracyclic [7. 75, 9. 4. 05, 10. 08, 9] octodecane from Taiwania cryptomerioidesim

2018 ◽  
Vol 16 (46) ◽  
pp. 9059-9063
Author(s):  
Wen-Li Wang ◽  
Dong-Rong Zhu ◽  
Jie Luo ◽  
Fei-Yan Liu ◽  
Chen Chen ◽  
...  
Keyword(s):  
Tetracyclic Core ◽  
Taiwania Cryptomerioides

Taiwanoids A–D (1–4) representing four dimeric diterpenoids featuring an uncommon tetracyclic core were isolated from the leaves of Taiwania cryptomerioides.

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