Urease production using corn steep liquor as a low-cost nutrient source by Sporosarcina pasteurii: biocementation and process optimization via artificial intelligence approaches

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

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Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: Process optimization and metabolic engineering

Bioresource Technology ◽

10.1016/j.biortech.2016.04.020 ◽

2016 ◽

Vol 212 ◽

pp. 100-110 ◽

Cited By ~ 42

Author(s):

Daiana Wischral ◽

Jianzhi Zhang ◽

Chi Cheng ◽

Meng Lin ◽

Lucas Monteiro Galotti De Souza ◽

...

Keyword(s):

Metabolic Engineering ◽

Process Optimization ◽

Crude Glycerol ◽

Corn Steep Liquor ◽

Clostridium Beijerinckii ◽

Steep Liquor

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Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

Sustainability ◽

10.3390/su12177122 ◽

2020 ◽

Vol 12 (17) ◽

pp. 7122

Author(s):

Ludwika Tomaszewska-Hetman ◽

Waldemar Rymowicz ◽

Anita Rywińska

Keyword(s):

Yarrowia Lipolytica ◽

Crude Glycerol ◽

Low Cost ◽

Corn Steep Liquor ◽

Culture Broth ◽

Biodiesel Production ◽

Innovative Strategy ◽

Simple Medium ◽

Low Cost Strategy ◽

Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

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Statistical Optimization of Acetoin Production Using Corn Steep Liquor as a Low-Cost Nitrogen Source by Bacillus Subtilis CICC 10025

Renewable Resources and Biorefineries ◽

10.5772/intechopen.79353 ◽

2019 ◽

Cited By ~ 1

Author(s):

Abiola Ezekiel Taiwo ◽

Tunde Victor Ojumu ◽

Tafirenyika N. Madzimbamuto

Keyword(s):

Bacillus Subtilis ◽

Nitrogen Source ◽

Low Cost ◽

Corn Steep Liquor ◽

Statistical Optimization ◽

Acetoin Production ◽

Steep Liquor

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Butanol production by Clostridium pasteurianum NRRL-598 using corn steep liquor as nutrient source

Brazilian Journal of Development ◽

10.34117/bjdv6n7-238 ◽

2020 ◽

Vol 6 (7) ◽

pp. 45399-45404

Author(s):

Larissa C. P. Ribeiro ◽

Maria Alice Z. Coelho ◽

Tatiana F. Ferreira

Keyword(s):

Corn Steep Liquor ◽

Clostridium Pasteurianum ◽

Butanol Production ◽

Nutrient Source ◽

Steep Liquor

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Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Biointerface Research in Applied Chemistry ◽

10.33263/briac103.348354 ◽

2020 ◽

Vol 10 (3) ◽

pp. 5348-5354

Keyword(s):

Crude Glycerol ◽

Low Cost ◽

Corn Steep Liquor ◽

Aeration Rate ◽

Phaffia Rhodozyma ◽

Agitation Rate ◽

Fed Batch ◽

Batch Bioreactor ◽

Steep Liquor ◽

Β Carotene

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

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