Evaluation of white Kwao Krua (Pueraria candollei Grah. ex Benth.) products sold in Thailand by molecular, chemical, and microscopic analyses

2019 ◽  
Vol 74 (1) ◽  
pp. 106-118 ◽  
Author(s):  
Aekkhaluck Intharuksa ◽  
Masashi Kitamura ◽  
Nichakan Peerakam ◽  
Wannaree Charoensup ◽  
Hirokazu Ando ◽  
...  
Keyword(s):  
Pueraria Candollei ◽  
White Kwao Krua

scholarly journals Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract

2015 ◽  
Vol 19 ◽  
pp. 269-277 ◽  
Author(s):  
Orapin Udomsin ◽  
Thaweesak Juengwatanatrakul ◽  
Gorawit Yusakul ◽  
Waraporn Putalun
Keyword(s):  
Crude Extract ◽  
Estrogenic Compounds ◽  
Pueraria Candollei ◽  
White Kwao Krua

An enzyme-linked immunoassay using anti-miroestrol monoclonal antibody for quality control of Pueraria candollei products

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
T Kitisripanya ◽  
J Komaikul ◽  
K Jutathis ◽  
O Udomsin ◽  
H Tanaka ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quality Control ◽  
Pueraria Candollei

Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation

2010 ◽  
Vol 33 (2) ◽  
pp. 369-374 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Pueraria Candollei

scholarly journals Transformation of Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity

RSC Advances ◽  
2021 ◽  
Vol 11 (51) ◽  
pp. 32067-32076
Author(s):  
Fonthip Makkliang ◽  
Wipawee Juengsanguanpornsuk ◽  
Suppalak Phaisan ◽  
Attapon Sakdamas ◽  
Waraporn Putalun ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Pueraria Candollei

Transformation of Pueraria candollei var. mirifica phytoestrogens by β-glucosidase increases estrogenic activity.

scholarly journals Characterization and validation of fragment antigen-binding (Fab) antibody-based immunoassay for deoxymiroestrol, a potent phytoestrogen from Pueraria candollei

2021 ◽  
Author(s):  
Worapol Sae-foo ◽  
Supaluk Krittanai ◽  
Wipawee Juengsanguanpornsuk ◽  
Gorawit Yusakul ◽  
Tharita Kitisripanya ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Estrogenic Activity ◽  
Limit Of Detection ◽  
Hormone Replacement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Binding ◽  
Pueraria Candollei ◽  
Specific Determination ◽  
Fragment Antigen Binding

Deoxymiroestrol is the most potent phytoestrogen in chromenes group that has been found in Pueraria candollei, Thai name known as Kwao Krua Khao. Several studies reported estrogenic activity of P. candollei in order to using as hormone replacement therapy for postmenopausal women. Previously, specific determination of deoxymiroestrol content by enzyme-linked immunosorbent assay (ELISA) using polyclonal antibody (pAb) have been reported. However, production of pAb has limitation and variability from different batches. Therefore, in this study, we established quantitative method for determination of deoxymiroestrol using fragment antigen-binding (Fab) antibody-based immunoassay. The developed immunoassay has specificity to deoxymiroestrol with a calibration range of 15.6-1000 ng mL-1. Precision including intra-assay and inter-assay are 1.48-7.11 and 0.58-9.31%, respectively. Accuracy of the assay showed in recovery between 99.77-101.61% when spike deoxymiroestrol standard into the samples. The limit of detection (LOD) is 30.80 ng mL-1. Comparation antibody-based immunoassay for determination of deoxymiroestrol using Fab with pAb was represented consistency (R2 = 0.9807) when analysis roots bark of Pueraria candollei from difference areas. Therefore, this development assay can apply to determine deoxymiroestrol content in the plant samples.

Norsesquiterpenes from the Roots of White Kwao Krua (Pueraria mirifica)

2014 ◽  
Vol 57 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Jung-Hwa Kwon ◽  
Jin-Gyeong Cho ◽  
Hee-Jung Park ◽  
Gyu-Won Huh ◽  
Myun-Ho Bang ◽  
...  
Keyword(s):  
Pueraria Mirifica ◽  
White Kwao Krua
Sign in / Sign up

Export Citation Format

Share Document