scholarly journals An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

2014 ◽  
Vol 20 (3) ◽  
pp. 405-410 ◽  
Author(s):  
J. J. Chavan ◽  
A. S. Nalawade ◽  
N. B. Gaikwad ◽  
R. V. Gurav ◽  
G. B. Dixit ◽  
...  
Author(s):  
Avinash A. Adsul ◽  
Jaykumar J. Chavan ◽  
Nikhil B. Gaikwad ◽  
Rajaram V. Gurav ◽  
Ghansham B. Dixit ◽  
...  

Abstract The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.


2018 ◽  
Vol 17 (1) ◽  
pp. 21
Author(s):  
Arun Kanagavel ◽  
Sethu Parvathy ◽  
Abhiijth P. Chundakatil ◽  
Neelesh Dahanukar ◽  
Benjamin Tapley

Distribution and habitat associations of the Critically Endangered frog Walkerana phrynoderma (Anura: Ranixalidae), with an assessment of potential threats, abundance, and morphology. Little is known about Walkerana phrynoderma, a frog endemic to the Anamalai Hills of the Western Ghats of India. Baseline information (i.e., distribution, threats, habitat characteristics, activity patterns, and relative abundance) is provided for this species, with the aim of improving our understanding of the status of the species in the wild. Visual-encounter, transect, and time-activity budget surveys were conducted in and around the Anamalai Hills of the Western Ghats. The frog skin was swabbed to determine the presence/absence of Batrachochytrium dendrobatidis, and habitat and environmental characteristics were recorded at sites where W. phrynoderma was found. These data were compared with those of sites apparently lacking this species that had suitable habitat. Walkerana phrynoderma is restricted to evergreen forests between 1300 and 1700 m a.s.l. in the Anamalai Tiger Reserve and at Munnar; thus, its range was extended from the state of Tamil Nadu to the adjoining state of Kerala. Pesticide runoff and human disturbance are the most severe threats to the species; B. dendrobatidis was not detected. This nocturnal anuran prefers forest edges and is associated with well-shaded forest foors in cool areas near freshwater streams. Walkerana phrynoderma is rarely encountered whereas its congener, W. leptodactyla, is more common. The impact of anthropogenic disturbances, especially waste disposal and development of tourism infrastructure, should be evaluated. The land that is owned by the Forest Department peripheral to the protected areas could be designated as eco-sensitive sites to prevent changes in land use that could have an adverse effect on W. phrynoderma.


2018 ◽  
Vol 9 ◽  
Author(s):  
S. Sai Latha ◽  
S. Naveen ◽  
C. K. Pradeep ◽  
C. Sivaraj ◽  
M. G. Dinesh ◽  
...  

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)


Phytotaxa ◽  
2017 ◽  
Vol 302 (2) ◽  
pp. 193 ◽  
Author(s):  
R. RAMASUBBU ◽  
C. DIVYA ◽  
N. SASI KALA ◽  
ANJANA SURENDRAN ◽  
A.K. SREEKALA

Impatiens megamalayana, a new species from Tamil Nadu, India is described and illustrated. This species is characterized by a ridged stem, ensiform and villous leaves, a wrinkled boat-shaped lower sepal with outwardly curved tip, ornamented seeds and strictly ovate pollen grains. This combination of characters makes determining the relationships of I. megamalayana difficult. The floral structure of I. megamalayana is closely allied to I. herbicola Hook.f. and I. inconspicua Benth. in Wall., the latter with three varieties, but it differs by several unique vegetative and floral features. Impatiens megamalayana is assessed as Critically Endangered in accordance with the IUCN guidelines. The habitat of the species is severely affected by landslides. As a result, effective strategies should be developed to protect I. megamalayana from exinction.


2012 ◽  
Vol 6 (4) ◽  
pp. 318-325 ◽  
Author(s):  
A.K. Gaur ◽  
Eti Sharma ◽  
Khushhaal Gaur ◽  
Himanshu Punetha

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