Adding gelling agents to cotton ovule culture media leads to subtle changes in fiber development

Effects of autoclaving volume, gelling agent (Bactoagar, Gel-gro, Phytagar), and basal salts [Murashige and Skoog (MS); Woody Plant Medium (WPM); Gamborg B5 (GB)] on gel strength and pH of tissue culture media were tested. Gel strength was significantly affected by gelling agent and basal medium. MS media were generally softer than comparable WPM or GB media. As the vessel volume during autoclaving decreased, gel strength significantly decreased with Phytagar and Bactoagar gelling agents; Gel-gro had greater gel strength at the intermediate volume of medium autoclave. In all cases, autoclaving resulted in a pH decrease of 0.2 to 0.5 pH units. Lower pH values were associated with softer gels. The type of gelling agent did not greatly affect the postautoclave pH; mean values among gelling agents were within 0.05 pH units. Postautoclave pH of MS medium was lower than that of WPM or GB. This study verifies the need to observe uniform sterilization protocols to maintain consistency in the chemical and physical properties of media.

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Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

International Journal of Biology ◽

10.5539/ijb.v11n4p26 ◽

2019 ◽

Vol 11 (4) ◽

pp. 26

Author(s):

Sai Krishna Repalli ◽

Chaitanya Kumar Geda ◽

N. S. N. Pradhan ◽

G. J. N. Rao

Keyword(s):

Culture Media ◽

Indica Rice ◽

Casein Hydrolysate ◽

Gelling Agent ◽

Regeneration Potential ◽

Rice Varieties ◽

Gelling Agents ◽

Profound Influence ◽

In Vitro Response

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.

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Admixture of Isubgol husk together with conventionally used agar as gelling agent for Potato and Stevia regeneration

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v47i2.11447 ◽

2012 ◽

Vol 47 (2) ◽

pp. 161-166

Author(s):

Salim Khan ◽

Abdullah Al Maruf ◽

Shahina Aktera ◽

Ahashan Habiba ◽

Tanjina Akhtar Banua ◽

...

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Culture Media ◽

Gelling Agent ◽

Plantago Ovata ◽

Gelling Agents ◽

Tissue Culture Media ◽

Commercially Important ◽

The Cost

Attempts have been made to find a substitute for agar as gelling agent. The study was conducted on commercially important crop plants like potato varieties (Diamond and Granola) and Stevia. 'Isubgol', the mucilaginous husk, derived from Plantago ovata was successfully used as a gelling material in tissue culture media. The price of 'Isubgol husk' is cheaper than the conventionally used agar and it had reduced the price of gelling agent approximately by 47.5 % in plant tissue culture media. The use of 'Isubgol husk' along with agar can reduce the cost of gelling agents. The response from media gelled with Isubgol husk in potato and Stevia was similar to that from media solidified with agar. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11447 Bangladesh J. Sci. Ind. Res. 47(2), 161-166, 2012

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Evaluation of gelling agents on anther culture: response of two soybean cultivars

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000700004 ◽

2007 ◽

Vol 50 (6) ◽

pp. 933-939 ◽

Cited By ~ 2

Author(s):

Milena Barcelos Cardoso ◽

Maria Helena Bodanese-Zanettini ◽

Elsa Cristina de Mundstock ◽

Eliane Kaltchuk-Santos

Keyword(s):

Developmental Stage ◽

Culture Media ◽

Callus Formation ◽

Gelling Agent ◽

Embryogenic Calli ◽

Gelling Agents ◽

Soybean Cultivars ◽

Viability Assay ◽

Significant Difference ◽

Anther Culture Response

Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies.

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