scholarly journals Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

2019 ◽  
Vol 11 (4) ◽  
pp. 26
Author(s):  
Sai Krishna Repalli ◽  
Chaitanya Kumar Geda ◽  
N. S. N. Pradhan ◽  
G. J. N. Rao
Keyword(s):  
Culture Media ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Gelling Agent ◽  
Regeneration Potential ◽  
Rice Varieties ◽  
Gelling Agents ◽  
Profound Influence ◽  
In Vitro Response

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.

scholarly journals In vitro response and effect of gamma irradiation on four local indica rice varieties

2020 ◽  
pp. 90-92
Author(s):  
Md. Monirul Islam ◽  
Md Taufiqur Rahman ◽  
Md. Hasanuzzaman ◽  
Md. Shahidul Islam ◽  
Md. Imtiaz Uddin ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Gamma Radiation ◽  
Gamma Rays ◽  
Callus Induction ◽  
Indica Rice ◽  
Callus Growth ◽  
Rice Varieties ◽  
Rice Callus ◽  
In Vitro Response

In vitro response of four local Indica rice cultivars viz. Sadamota, Kachamota, Moulata and Dudhkalam was evaluated. The aim of this study is to develop an efficient protocol for callus induction, plant regeneration and to observe the effect of gamma radiation on plant regeneration for creating possible genetic variability. In Different concentration of 2,4-D and growth regulators were supplemented with MS medium (Murashige and Skoog’s) to observe their callus induction frequency using mature embryo as explant. Among the cutivars, the highest primary callus (92.55%) as well as embryogenic callus induction (56.26%) was showed in sadamota at 3.0 mgl-1 2,4-D and 10 mgl-1 kinetin under dark condition. Twenty one days old embryogenic calli were exposed to 0, 2, 4 and 6 Gy of gamma rays and transferred to regeneration medium. Both callus growth and regeneration capacity were found to be decreased with increasing level of exposure to gamma rays. The doses of 4 Gy of gamma radiation were found to be the 50% inhibition dose for callus growth and plant regeneration in sadamota and kachamota, repectively whereas the 50% inhibition dose for moulata and dudhkalam at 2 Gy. This results indicate that sentivity of gamma radiation on rice callus depends on genotype of a genus.

scholarly journals Studies on in vitro response to callus induction and gene transfer technique of five high yielding indica rice varieties.

1970 ◽  
pp. 41-45 ◽  
Author(s):  
Md. Niuz Morshed Khan ◽  
Dr. Md. Monirul Islam ◽  
Dr. Md. Shahidul Islam
Keyword(s):  
Gene Transfer ◽  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Induction Medium ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
In Vitro Response ◽  
Increasing Demand

Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64were observed that is done in four different concentration of growth regulators (2,4-D). The highest callus induction were observed in media containing 2 mg/L 2,4-D. Among those varieties BRRI dhan-48 showed highest rate of callus induction. In this experiment Agrobacterium mediated gene transfer is also tried although plants were found nut need to be tested by molecular methods and tested the desired characters for selecting those plantlets as transgenic plant. This study will be useful for selecting suitable callus induction medium for callus induction in future.

scholarly journals In vitro answer of Bulgarian pepper (Capsicum annuum L.)

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 129-136
Author(s):  
Velichka Rodeva ◽  
Stanislava Grozeva ◽  
Velichka Todorova
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Casein Hydrolysate ◽  
Basal Medium ◽  
Regeneration Ability ◽  
Stem Explants ◽  
Media Composition ◽  
Breeding Lines ◽  
High Level

Callusogenesis and regeneration ability of cotyledon and hypocotyl explants from three Bulgarian pepper varieties in MS basal medium supplemented with l-3mg/l BAP. l.0mg/1 IAA and 0.5mg/l GA3 was studied. In the different variants of culture medium was registered high level of callusogenesis and organogenesis in both type of explants from the all varieties. The highest percentage of plant-regenerants is established in cotyledon explants (from 3.3 to 18.3) in variant 3 of the culture medium containing 3mg/l BA. In the process of micropropagation by stem explants of the same studied pepper varieties the addition of the vitamins C. B12. Casein hydrolysate and Ferulic acid had a stimulation effect on the plant growth in height and rooting. In result of anther cultivation from three pepper varieties and four breeding lines the highest percentage of embryo structure formation was registered in varieties Albena and Strjama (12.0 and 13.8 respectively). The Bulgarian peppers are recalcitrant and their in vitro answer is different depending from the explants type, genotype and the culture media composition.

scholarly journals Establishment of Morphogenic and Regeneration Ability in two Indica Rice Cell Suspension Cultures after Exposure to NaCl

1970 ◽  
Vol 19 (2) ◽  
pp. 185-197
Author(s):  
T.L. Aditya
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Indica Rice ◽  
Cell Suspension Cultures ◽  
Nacl Stress ◽  
Callus Cultures ◽  
Regeneration Ability ◽  
Rice Varieties

An efficient protocol was developed for in vitro morphogenic ability along with plantlet regeneration of two Bangladeshi indica rice varieties (BR24 and BR26) via somatic embryogenesis by applying 50 mM NaCl stress in callus induction and suspension initiation media. Osmotic stress was induced by NaCl (50, 100, 150, 200 and 250 mM) on the cell growth in suspension maintenance media. In viability test stress adapted cells showed 85 - 95% viability up to 200 mM NaCl compared with stress shocked (MS1-50) and control (MS1-0) treatments. Higher stress adapted cells showed growth retardation and the induction of plasmolysis. For both genotypes somatic embryos were obtained in both MS based liquid and semisolid media with or without 50 and 100 mM NaCl. Cell suspension-derived micro-calli were partially desiccated (6 - 12 hr) and subsequently maintained in MS1 callus induction media supplemented with proline (12 mM), ABA (2 mg/l) and 0.6% phytagel in the presence or absence of 50 and 100 mM NaCl. Subsequently, desiccated somatic embryos were transferred in MS based regeneration media with or without 50 and 100 mM NaCl. Proline mediated callus was found to be more effective in embryo differentiation than ABA. Partial desiccation dramatically enhanced callus growth and partially increased regeneration percentage. BR24 showed a better regeneration response producing plantlets in presence of proline in control media while BR26 restored regeneration potential in the presence of ABA and 100 mM NaCl. Plantlets regenerated from salt stressed callus cultures were then grown in compost in a glasshouse and produced normal, fertile plants.  Key words: Indica rice, Cell suspension, Morphogenic, Regeneration D.O.I. 10.3329/ptcb.v19i2.5436 Plant Tissue Cult. & Biotech. 19(2): 185-197, 2009 (December)

scholarly journals Embryo rescue from interspecific crosses in apple rootstocks

2006 ◽  
Vol 41 (6) ◽  
pp. 969-973 ◽  
Author(s):  
Adriana Cibele de Mesquita Dantas ◽  
José Itamar Boneti ◽  
Rubens Onofre Nodari ◽  
Miguel Pedro Guerra
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Embryo Rescue ◽  
Casein Hydrolysate ◽  
Female Parent ◽  
Interspecific Crosses ◽  
Open Pollination ◽  
Apple Rootstocks ◽  
Hand Pollination

The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings.

scholarly journals Vessel Volume, Gelling Agent, and Basal Salts Affect pH and Gel Strength of Autoclave Tissue Culture Media

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 683-685 ◽  
Author(s):  
Hazel Y. Wetzstein ◽  
Choongsik Kim ◽  
Harry E. Sommer
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Basal Medium ◽  
Gel Strength ◽  
Gelling Agent ◽  
Mean Values ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Vessel Volume ◽  
Basal Salts

Effects of autoclaving volume, gelling agent (Bactoagar, Gel-gro, Phytagar), and basal salts [Murashige and Skoog (MS); Woody Plant Medium (WPM); Gamborg B5 (GB)] on gel strength and pH of tissue culture media were tested. Gel strength was significantly affected by gelling agent and basal medium. MS media were generally softer than comparable WPM or GB media. As the vessel volume during autoclaving decreased, gel strength significantly decreased with Phytagar and Bactoagar gelling agents; Gel-gro had greater gel strength at the intermediate volume of medium autoclave. In all cases, autoclaving resulted in a pH decrease of 0.2 to 0.5 pH units. Lower pH values were associated with softer gels. The type of gelling agent did not greatly affect the postautoclave pH; mean values among gelling agents were within 0.05 pH units. Postautoclave pH of MS medium was lower than that of WPM or GB. This study verifies the need to observe uniform sterilization protocols to maintain consistency in the chemical and physical properties of media.

scholarly journals Effect of gelling agents on in vitro development of Amelanchier canadensis ‘Rainbow Pillar’

2013 ◽  
Vol 19 (3-4) ◽  
Author(s):  
A. Fira ◽  
K. Magyar-Tábori ◽  
I. Hudák ◽  
D. Clapa ◽  
J. Dobránszky
Keyword(s):  
Guar Gum ◽  
Growth Parameters ◽  
Shoot Multiplication ◽  
Wheat Starch ◽  
Gelling Agent ◽  
Shoot Cultures ◽  
Gelling Agents ◽  
Shoot Number ◽  
The Media

In vitro shoot multiplication responses of Amelanchier canadensis ‘Rainbow Pillar’ were studied on media solidifi ed with different gelling agents. The media were gelled either with 6.8 g l-1 fi brous agar-agar, or 50.0 g l-1 wheat starch, or 20.0 g l-1 Guar gum, or 15 g l-1 Isubgol or 50.0 g l-1 wheat starch mixed with 0.5 g l-1 Phytagel. Shoot cultures were grown for two months, thereafter the multiplication rates (number of newly developed shoots per explant) were counted and the length of shoots were measured. We found that the highest shoot multiplication of Amelanchier canadensis ‘Rainbow Pillar’ occurred on media gelled with Guar gum, while the longest shoots developed on media with Starch. About four-fold shoot number were obtained on media with Guar gum compared to the weakest results found on media gelled with Isubgol. Finally, considering all factors (shoot growth parameters, costs) the most economical gelling agent for Amelanchier canadensis ‘Rainbow Pillar’ was proved to be wheat starch among the tested alternatives which allows a 75.6% cost reduction.

scholarly journals Plant Regeneration Efficiency of Two Scented Indica Rice Varieties Pusa Basmati 1 and Kalanamak

2013 ◽  
Vol 22 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Aniruddha Kumar Tiwari ◽  
Md Shamim ◽  
Ravi Prakash Saxena ◽  
Kapil Deo Narayan Singh
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Growth Hormones ◽  
Rice Varieties ◽  
Shoot Initiation ◽  
Seed Embryo ◽  
Regeneration Efficiency

This study was undertaken to establish a regeneration protocol for two scented indica rice varieties, namely Pusa Basmati1 and Kalanamak. Callus culture in Pusa Basmati1 was initiated in MS containing 2.0 mg/l of 2,4-D. Optimum requirement of growth hormones for callus induction in Kalanamak was 1.5 mg/l of 2,4-D supplemented with 0.1 mg/l each of NAA and BAP in MS. Shoot regeneration in Pusa Basmati1 initiated in MS containing 2.0 mg/l BAP, 0.5 mg/l each of NAA and Kn. There was no shoot initiation in MS with same composition in Kalanamak. The shoot regeneration was successfully initiated and achieved in Dl medium supplemented with 2.0 mg/l BAP, 0.2 mg/l each of IAA, NAA and Kn, 500 mg/l each of proline and glutamine and 800 mg/l casein hydrolysate. The calli derived from mature seed embryo produced fertile green plants. The plants were successfully transferred to field with normal flowering. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14206 Plant Tissue Cult. & Biotech. 22(2): 163-169, 2012 (December)

scholarly journals Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

2019 ◽  
Vol 7 ◽  
pp. 97-104
Author(s):  
Md. Niuz Morshed Khan ◽  
Md. Monirul Islam ◽  
Dr. Md. Shahidul Islam
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Induction Medium ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
New Variety ◽  
Increasing Demand ◽  
Embryogenic Callus Formation

Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

scholarly journals Admixture of Isubgol husk together with conventionally used agar as gelling agent for Potato and Stevia regeneration

2012 ◽  
Vol 47 (2) ◽  
pp. 161-166
Author(s):  
Salim Khan ◽  
Abdullah Al Maruf ◽  
Shahina Aktera ◽  
Ahashan Habiba ◽  
Tanjina Akhtar Banua ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Media ◽  
Gelling Agent ◽  
Plantago Ovata ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Commercially Important ◽  
The Cost

Attempts have been made to find a substitute for agar as gelling agent. The study was conducted on commercially important crop plants like    potato varieties (Diamond and Granola) and Stevia. 'Isubgol', the mucilaginous husk, derived from Plantago ovata was successfully used as a gelling material in tissue culture media. The price of 'Isubgol husk' is cheaper than the conventionally used agar and it had reduced the price of gelling agent approximately by 47.5 % in plant tissue culture media. The use of 'Isubgol husk' along with agar can reduce the cost of gelling agents. The response from media gelled with Isubgol husk in potato and Stevia was similar to that from media solidified with agar. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11447 Bangladesh J. Sci. Ind. Res. 47(2), 161-166, 2012

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