An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L.

2008 ◽  
Vol 30 (4) ◽  
pp. 493-499 ◽  
Author(s):  
Iram Siddique ◽  
M. Anis
Keyword(s):  
Plant Regeneration ◽  
Ocimum Basilicum ◽  
Ex Vitro ◽  
Regeneration System

scholarly journals An Efficient Tissue Culture Regeneration System for Georgia Plume, Elliottia racemosa, a Threatened Georgia Endemic

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 447-453 ◽  
Author(s):  
Seong Min Woo ◽  
Hazel Y. Wetzstein
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Dry Weight ◽  
Medium Composition ◽  
Induction Medium ◽  
Ex Vitro ◽  
Regeneration System

Georgia plume, Elliottia racemosa Muhlenb. ex. Elliott, is an extremely rare small tree or shrub endemic to Georgia, which is being severely affected by habitat loss and lack of sexual recruitment. In vitro plant regeneration of Georgia plume has not been previously reported and may be a method for the conservation and propagation of this threatened species. Studies evaluated the effects of sterilization methods, explant types, medium composition, and light environment on plant regeneration. An efficient plant regeneration system was developed in which adventitious shoot buds were induced using young, expanding leaf explants placed on an induction medium supplemented with 10 μm thidiazuron and 5 μm indole-3-acetic acid with Gamborg's B5 salts. Shoot elongation was promoted on a medium with 25 μm (2-isopentenyl) adenine incorporated into Woody Plant Medium. In vitro rooting studies evaluated continuous and pulse auxin treatments and ex vitro rooting trials after KIBA (indole-3-butric acid, potassium salt) dips. A 5-day pulse treatment on 100 or 150 μm indole-3-butyric acid produced ≈90% rooting of shoots with greater shoot and root dry weight than other pulse times. High rooting frequencies were obtained under in vitro and ex vitro conditions with over 85% survival of plantlets transferred to greenhouse conditions. The culture protocol was found to be effective with material collected from mature specimens in the wild from divergent populations. Tissue culture appears to be a promising approach for the propagation and conservation of this rare and threatened plant.

Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

2018 ◽  
Vol 93 (4) ◽  
pp. 1285-1295 ◽  
Author(s):  
Afshan Naaz ◽  
Sheikh Altaf Hussain ◽  
Ruphi Naz ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar
Keyword(s):  
Plant Regeneration ◽  
De Novo ◽  
Syzygium Cumini ◽  
Regeneration System ◽  
Medicinal Tree

An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba

2010 ◽  
Vol 46 (5) ◽  
pp. 445-450 ◽  
Author(s):  
Junli Wang ◽  
Jue Wang ◽  
Kun Liu ◽  
Xuan Xiao ◽  
Weizhen Gong ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Regeneration System ◽  
Flavonoid Accumulation

scholarly journals In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 318
Author(s):  
Mehtab Muhammad Aslam ◽  
Joseph K. Karanja ◽  
Qian Zhang ◽  
Huifeng Lin ◽  
Tianyu Xia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Genetic Engineering ◽  
Shoot Regeneration ◽  
Lupinus Albus ◽  
Cotyledonary Node ◽  
White Lupin ◽  
Regeneration System ◽  
Regeneration Frequency ◽  
Cotyledonary Nodes

The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

Establishment of an efficient plant regeneration system of dodder

2008 ◽  
Vol 136 ◽  
pp. S154-S155
Author(s):  
Dongxiao Li ◽  
Jingling Chang ◽  
Guoguang Zhang ◽  
Liang Chen
Keyword(s):  
Plant Regeneration ◽  
Regeneration System
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