Isolation of cold stress-responsive genes from Lepidium latifolium by suppressive subtraction hybridization

2009 ◽  
Vol 32 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Mohammad Aslam ◽  
Vimlendu B. Sinha ◽  
Rupesh K. Singh ◽  
Sivalingam Anandhan ◽  
Veena Pande ◽  
...  
Keyword(s):  
Cold Stress ◽  
Lepidium Latifolium ◽  
Subtraction Hybridization ◽  
Suppressive Subtraction Hybridization ◽  
Stress Responsive Genes

scholarly journals Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1706
Author(s):  
Mei Yang ◽  
Xinhang Duan ◽  
Zhaoyu Wang ◽  
Hang Yin ◽  
Junrui Zang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Cold Stress ◽  
Medicago Sativa ◽  
Transgenic Tobacco ◽  
Soluble Sugars ◽  
Ros Scavenging ◽  
Osmotic Homeostasis ◽  
Voltage Dependent ◽  
Stress Responsive Genes

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel VDAC gene was isolated and identified from alfalfa (Medicago sativa L.). MsVDAC localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of MsVDAC in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O2− content increased. Stress-responsive genes including LTP1, ERD10B and Hxk3 were upregulated in the transgenic plants under cold and drought stress. However, GAPC, CBL1, BI-1, Cu/ZnSOD and MnSOD were upregulated only in the transgenic tobacco plants under cold stress, and GAPC, CBL1, and BI-1 were downregulated under drought stress. These results suggest that MsVDAC provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via MsVDAC may be mainly due to osmotic homeostasis and stress-responsive genes.

scholarly journals Analysis of Genes Expressed During Porcine Fetal Pituitary Development by Suppressive Subtraction Hybridization

2007 ◽  
Vol 53 (5) ◽  
pp. 1087-1091 ◽  
Author(s):  
Jun TAKAHASHI ◽  
Takao SUSA ◽  
Takanobu SATO ◽  
Hiroko ASANO ◽  
Takako KATO ◽  
...  
Keyword(s):  
Subtraction Hybridization ◽  
Pituitary Development ◽  
Suppressive Subtraction Hybridization
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