Salicylic acid induces tolerance of Vitisriparia×V.labrusca to chilling stress by altered photosynthetic, antioxidant mechanisms and expression of cold stress responsive genes

2021 ◽  
Author(s):  
Bin Li ◽  
Wangtian Wang
Keyword(s):  
Salicylic Acid ◽  
Cold Stress ◽  
Chilling Stress ◽  
Stress Responsive Genes ◽  
Antioxidant Mechanisms

Acetyl salicylic acid alleviates chilling-induced damage in muskmelon seedlings

2007 ◽  
Vol 87 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Ahmet Korkmaz ◽  
Murat Uzunlu ◽  
Ali Riza Demirkiran
Keyword(s):  
Salicylic Acid ◽  
Cucumis Melo ◽  
Chilling Stress ◽  
Foliar Spray ◽  
Dry Weight ◽  
Acetyl Salicylic Acid ◽  
Signal Molecule ◽  
Stress Indicator ◽  
Shoot Dry Weight ◽  
Significant Difference

Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether acetyl salicylic acid (ASA) application at various concentrations through seed immersion or foliar spray would protect muskmelon [Cucumis melo L. (Reticulatus Group)] seedlings subjected to chilling stress. Twenty-one-day-old plants pre-treated with ASA (0, 0.1, 0.25, 0.50 or 1.0 mM) were subjected to chilling stress for 72 h at 3 ± 0.5°C. ASA, applied either through seed immersion or foliar spray, was effective within the range of 0.1 to 1 mM in inducing tolerance to chilling stress in muskmelon seedlings; however, there was no significant difference between application methods. ASA significantly and curvilinearly affected all seedling growth and stress indicator variables tested except shoot dry weight. The best protection was obtained from seedlings pre-treated with 0.5 mM ASA. The highest ASA concentration used was slightly less effective in providing chilling stress protection. Even though both methods provided similar means of protection, due to its simplicity and practicality, immersion of muskmelon seeds prior to sowing in 0.5 mM ASA would be a more desirable method to induce tolerance to chilling stress. Key words: Cucumis melo, aspirin, chilling stress tolerance, gas exchange, electrolyte leakage

scholarly journals Reactive Oxygen Species and the Redox-Regulatory Network in Cold Stress Acclimation

Antioxidants ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 169 ◽  
Author(s):  
Anna Dreyer ◽  
Karl-Josef Dietz
Keyword(s):  
Reactive Oxygen Species ◽  
Cold Stress ◽  
Regulatory Network ◽  
Chilling Stress ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Reactive Oxygen ◽  
Cold Climate ◽  
Oxygen Species ◽  
Cold Temperatures ◽  
Stress Acclimation

Cold temperatures restrict plant growth, geographical extension of plant species, and agricultural practices. This review deals with cold stress above freezing temperatures often defined as chilling stress. It focuses on the redox regulatory network of the cell under cold temperature conditions. Reactive oxygen species (ROS) function as the final electron sink in this network which consists of redox input elements, transmitters, targets, and sensors. Following an introduction to the critical network components which include nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductases, thioredoxins, and peroxiredoxins, typical laboratory experiments for cold stress investigations will be described. Short term transcriptome and metabolome analyses allow for dissecting the early responses of network components and complement the vast data sets dealing with changes in the antioxidant system and ROS. This review gives examples of how such information may be integrated to advance our knowledge on the response and function of the redox regulatory network in cold stress acclimation. It will be exemplarily shown that targeting the redox network might be beneficial and supportive to improve cold stress acclimation and plant yield in cold climate.

scholarly journals Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1706
Author(s):  
Mei Yang ◽  
Xinhang Duan ◽  
Zhaoyu Wang ◽  
Hang Yin ◽  
Junrui Zang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Cold Stress ◽  
Medicago Sativa ◽  
Transgenic Tobacco ◽  
Soluble Sugars ◽  
Ros Scavenging ◽  
Osmotic Homeostasis ◽  
Voltage Dependent ◽  
Stress Responsive Genes

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel VDAC gene was isolated and identified from alfalfa (Medicago sativa L.). MsVDAC localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of MsVDAC in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O2− content increased. Stress-responsive genes including LTP1, ERD10B and Hxk3 were upregulated in the transgenic plants under cold and drought stress. However, GAPC, CBL1, BI-1, Cu/ZnSOD and MnSOD were upregulated only in the transgenic tobacco plants under cold stress, and GAPC, CBL1, and BI-1 were downregulated under drought stress. These results suggest that MsVDAC provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via MsVDAC may be mainly due to osmotic homeostasis and stress-responsive genes.

scholarly journals Roles of Glutathione s-transferease in maize (Zea mays L.) under cold stress

2015 ◽  
Vol 2 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Md Mahfuzur Rahman ◽  
MA Khaleque Mian ◽  
Asgar Ahmed ◽  
Md Motiar Rohman
Keyword(s):  
Cold Stress ◽  
Redox State ◽  
Antioxidant Defense ◽  
Stress Condition ◽  
Chilling Stress ◽  
Protective Role ◽  
Glutathione S Transferase ◽  
Oxidative Damages ◽  
Organic Hydroperoxides ◽  
Hybrid Maize

Glutathione S-transferease (GST) activities involved in antioxidant defense and methylglyoxal detoxification were investigated in the seedlings of a Bangladeshi maize variety, BARI hybrid maize-7, to understand the protecting mechanism under cold stress condition. The activities of glutathione S-transferase (GST) increased, while the activities of catalase (CAT) decreased with the duration of stress. The western blot analysis of the dominant GST revealed that it significantly accumulated during the stress period. The continual increase in H2O2 contents along with reduced redox state and activities suggested their roles in maintaining the glutathione homeostasis. The accumulation of GST with the content of H2O2 suggested its detoxification roles for organic hydroperoxides during chilling stress. Considering all, glutathione S-transferase (GST) enzymes showed protective role in maize from oxidative damages under Chilling condition.Res. Agric., Livest. Fish.2(1): 9-15, April 2015

Salicylic acid analogues with biological activity may induce chilling tolerance of maize (Zea mays) seeds

Botany ◽  
2012 ◽  
Vol 90 (9) ◽  
pp. 845-855 ◽  
Author(s):  
Yang Wang ◽  
Jin Hu ◽  
Guochen Qin ◽  
Huawei Cui ◽  
Qitian Wang
Keyword(s):  
Zea Mays ◽  
Biological Activity ◽  
Salicylic Acid ◽  
Chilling Stress ◽  
Chilling Tolerance ◽  
Biologically Active ◽  
Aminobenzoic Acid ◽  
Aminosalicylic Acid ◽  
Shoot Height ◽  
Maize Seeds

One kind of biologically active salicylic acid (SA) analogue (acetylsalicylic acid, ASA) and two inactive compounds (4-aminosalicylic acid and 4-aminobenzoic acid), along with SA were chosen to evaluate their role in inducing chilling tolerance of two different chilling-tolerant maize ( Zea mays L.) inbred lines. These compounds were applied as seed treatments or as a hydroponic application. The results showed that four compounds had no significant effect on germination of maize seeds; however, SA or ASA soaking treatments significantly increased the root length, shoot height, and shoot and root dry weights of seedlings grown under chilling stress. Hydroponic applications of SA or ASA significantly alleviated the accumulation of malondialdehyde, hydrogen peroxide, and superoxide radicals in roots and leaves of both lines under chilling stress, and the applications also increased the photosynthetic pigments, including chlorophyll a, chlorophyll b, and carotenoids. However, 4-aminosalicylic acid and 4-aminobenzoic acid applications had no significant effect in ameliorating the growth inhibition of seedlings under chilling stress. This study showed that SA and ASA significantly induced the chilling tolerance of maize; however, 4-aminosalicylic acid and 4-aminobenzoic acid were not effective in inducing tolerance to chilling stress. The results suggest that only SA analogues with biological activity may have the ability to induce chilling tolerance of maize.

scholarly journals The effect of salicylic acid on cell membrane stability in in vitro micropropagated tea plants under cold stress

2019 ◽  
pp. 110-117
Author(s):  
L. S. Samarina ◽  
M. V. Gvasaliya ◽  
L. S. Maluykova ◽  
V. I. Malyarovskaya ◽  
R. S. Rakhmangulov ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Cell Membrane ◽  
Cold Stress ◽  
Membrane Stability ◽  
Cell Membrane Stability ◽  
Tea Plants

scholarly journals Salicylic Acid Is Involved in Rootstock–Scion Communication in Improving the Chilling Tolerance of Grafted Cucumber

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Fu ◽  
Yi-Qing Feng ◽  
Xiao-Wei Zhang ◽  
Yan-Yan Zhang ◽  
Huan-Gai Bi ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Root Zone ◽  
Chilling Stress ◽  
Photochemical Efficiency ◽  
Chilling Tolerance ◽  
Rubisco Activase ◽  
Protein Levels ◽  
Binding Factor ◽  
Cold Tolerant ◽  
Cold Sensitive

Salicylic acid (SA) has been proven to be a multifunctional signaling molecule that participates in the response of plants to abiotic stresses. In this study, we used cold-sensitive cucumber and cold-tolerant pumpkin as experimental materials to examine the roles of SA in root–shoot communication responses to aerial or/and root-zone chilling stress in own-root and hetero-root grafted cucumber and pumpkin plants. The results showed that pumpkin (Cm) rootstock enhanced the chilling tolerance of grafted cucumber, as evidenced by the observed lower levels of electrolyte leakage (EL), malondialdehyde (MDA), and higher photosynthetic rate (Pn) and gene expression of Rubisco activase (RCA). However, cucumber (Cs) rootstock decreased the chilling tolerance of grafted pumpkins. Cs/Cm plants showed an increase in the mRNA expression of C-repeat-binding factor (CBF1), an inducer of CBF expression (ICE1), and cold-responsive (COR47) genes and CBF1 protein levels in leaves under 5/25 and 5/5°C stresses, or in roots under 25/5 and 5/5°C stresses, respectively, compared with the Cs/Cs. Chilling stress increased the endogenous SA content and the activity of phenylalanine ammonia-lyase (PAL), and the increase in SA content and activity of PAL in Cs/Cm plants was much higher than in Cs/Cs plants. Transcription profiling analysis revealed the key genes of SA biosynthesis, PAL, ICS, and SABP2 were upregulated, while SAMT, the key gene of SA degradation, was downregulated in Cs/Cm leaves, compared with Cs/Cs leaves under chilling stress. The accumulation of SA in the Cs/Cm leaves was mainly attributed to an increase in SA biosynthesis in leaves and that in transport from roots under aerial and root-zone chilling stress, respectively. In addition, exogenous SA significantly upregulated the expression level of cold-responsive (COR) genes, enhanced actual photochemical efficiency (ΦPSII), maximum photochemical efficiency (Fv/Fm), and Pn, while decreased EL, MDA, and CI in grafted cucumber. These results suggest that SA is involved in rootstock–scion communication and grafting-induced chilling tolerance by upregulating the expression of COR genes in cucumber plants under chilling stress.

scholarly journals Spatial–Temporal Response of Reactive Oxygen Species and Salicylic Acid Suggest Their Interaction in Pumpkin Rootstock-Induced Chilling Tolerance in Watermelon Plants

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2024
Author(s):  
Fei Cheng ◽  
Min Gao ◽  
Junyang Lu ◽  
Yuan Huang ◽  
Zhilong Bie
Keyword(s):  
Salicylic Acid ◽  
Chilling Stress ◽  
Redox Homeostasis ◽  
Early Response ◽  
Chilling Tolerance ◽  
Plant Tolerance ◽  
Temporal Response ◽  
Signal Molecule ◽  
Antioxidant Metabolism ◽  
Psii Activity

Grafting with pumpkin rootstock could improve chilling tolerance in watermelon, and salicylic acid (SA) as a signal molecule is involved in regulating plant tolerance to chilling and other abiotic stresses. To clarify the mechanism in pumpkin rootstock-induced systemic acquired acclimation in grafted watermelon under chilling stress, we used self-grafted (Cl/Cl) and pumpkin rootstock-grafted (Cl/Cm) watermelon seedlings to study the changes in lipid peroxidation, photosystem II (PSII) activity and antioxidant metabolism, the spatio–temporal response of SA biosynthesis and H2O2 accumulation to chilling, and the role of H2O2 signal in SA-induced chilling tolerance in grafted watermelon. The results showed that pumpkin rootstock grafting promoted SA biosynthesis in the watermelon scions. Chilling induced hydrolysis of conjugated SA into free SA in the roots and accumulation of free SA in the leaves in Cl/Cm plants. Further, pumpkin rootstock grafting induced early response of antioxidant enzyme system in the roots and increased activities of ascorbate peroxidase and glutathione reductase in the leaves, thus maintaining cellular redox homeostasis. Exogenous SA improved while the inhibition of SA biosynthesis reduced chilling tolerance in Cl/Cl seedlings. The application of diphenyleneiodonium (DPI, inhibitor of NADPH oxidase) and dimethylthiourea (DMTU, H2O2 scavenger) decreased, while exogenous H2O2 improved the PSII activity in Cl/Cl plants under chilling stress. Additionally, the decrease of the net photosynthetic rate in DMTU- and DPI-pretreated Cl/Cl plants under chilling conditions could be alleviated by subsequent application of H2O2 but not SA. In conclusion, pumpkin rootstock grafting induces SA biosynthesis and redistribution in the leaves and roots and participates in the regulation of antioxidant metabolism probably through interaction with the H2O2 signal, thus improving chilling tolerance in watermelon.

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