Sodium azide mutagenesis within temporary immersion bioreactors modifies sugarcane in vitro micropropagation rates and aldehyde, chlorophyll, carotenoid, and phenolic profiles

2019 ◽  
Vol 41 (7) ◽  
Author(s):  
Daviel Gómez ◽  
Lázaro Hernández ◽  
Julia Martínez ◽  
Doris Escalante ◽  
Byron E. Zevallos ◽  
...  
Keyword(s):  
Sodium Azide ◽  
Temporary Immersion ◽  
In Vitro Micropropagation ◽  
Phenolic Profiles

Comparison of different in vitro micropropagation methods of Stevia rebaudiana B. including temporary immersion bioreactor (BIT®)

2017 ◽  
Vol 131 (1) ◽  
pp. 195-199 ◽  
Author(s):  
Karel Vives ◽  
Iván Andújar ◽  
José Carlos Lorenzo ◽  
Oscar Concepción ◽  
Martha Hernández ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Micropropagation

scholarly journals In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production

2021 ◽  
Vol 14 (8) ◽  
pp. 747
Author(s):  
Eder Villegas Sánchez ◽  
Mariana Macías-Alonso ◽  
Soraya Osegueda Robles ◽  
Lisset Herrera-Isidrón ◽  
Hector Nuñez-Palenius ◽  
...  
Keyword(s):  
High Performance ◽  
Emerging Infectious Diseases ◽  
Rosmarinus Officinalis ◽  
Wild Plant ◽  
Array Detector ◽  
Phase System ◽  
Naphthaleneacetic Acid ◽  
Temporary Immersion System ◽  
Temporary Immersion

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

scholarly journals Data on recurrent somatic embryogenesis and in vitro micropropagation of Cnidium officinale Makino

Data in Brief ◽  
2018 ◽  
Vol 19 ◽  
pp. 2311-2314
Author(s):  
Muhammad Adil ◽  
Dong Il Kang ◽  
Byoung Ryong Jeong
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Micropropagation ◽  
Cnidium Officinale

scholarly journals A temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis

2011 ◽  
Vol 108 (8) ◽  
pp. 1463-1475 ◽  
Author(s):  
D. A. Steinmacher ◽  
M. P. Guerra ◽  
K. Saare-Surminski ◽  
R. Lieberei
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Secondary Somatic Embryogenesis ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Peach Palm

scholarly journals In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia

2014 ◽  
Vol 57 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Ummi Nur Ain Abdul Razak ◽  
Chong Boon Ong ◽  
Tiew Sing Yu ◽  
Li Kiaw Lau
Keyword(s):  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni ◽  
In Vitro Micropropagation

In vitro absorption of bile salts by small intestine of rats and guinea pigs

1961 ◽  
Vol 200 (2) ◽  
pp. 313-317 ◽  
Author(s):  
Leon Lack ◽  
I. M. Weiner
Keyword(s):  
Small Intestine ◽  
Bile Acid ◽  
Active Transport ◽  
Sodium Azide ◽  
Concentration Gradient ◽  
Guinea Pigs ◽  
Bile Salts ◽  
Ileal Segment

The transport of taurocholic and glycocholic acids by the small intestine of rats and guinea pigs against a concentration gradient was studied by the everted gutsac technique. Transport of these substances is limited to the distal ileal segment. This transport is inhibited by anoxia, dinitrophenol and sodium azide. The system has a transport maximum. On the basis of these criteria bile acid reabsorption is considered to occur by active transport.

EFFECT OF BA AND NAA ON IN VITRO MICROPROPAGATION OF TEA TREE

2014 ◽  
pp. 233-240
Author(s):  
A. Jala ◽  
W. Hankamolsiri
Keyword(s):  
Tea Tree ◽  
In Vitro Micropropagation
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