Morphogenetic potential and in vitro micropropagation of endangered plant species Leucojum aestivum L. and Lilium rhodopaeum Delip.

1994 ◽  
Vol 13 (8) ◽  
Author(s):  
MarinaI. Stanilova ◽  
ValentinaP. Ilcheva ◽  
NedjalkaA. Zagorska
Keyword(s):  
Plant Species ◽  
Endangered Plant ◽  
Endangered Plant Species ◽  
Leucojum Aestivum ◽  
In Vitro Micropropagation ◽  
Morphogenetic Potential

scholarly journals Protocol for efficient micropropagation of spring gentian and sand jurinea

2014 ◽  
Vol 60 (No. 1) ◽  
pp. 1-5
Author(s):  
H. Cvrčková ◽  
P. Máchová ◽  
J. Dostál ◽  
J. Malá
Keyword(s):  
Czech Republic ◽  
Plant Species ◽  
In Vitro Propagation ◽  
Casein Hydrolysate ◽  
Endangered Plant ◽  
Ms Medium ◽  
The Czech Republic ◽  
Endangered Plant Species ◽  
Primary Explants

Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg&middot;l<sup>&ndash;1</sup>of glutamine, 200 mg&middot;l<sup>&ndash;1 </sup>of casein hydrolysate, 30 g&middot;l<sup>&ndash;1 </sup>of sucrose, 0.2 mg&middot;l<sup>&ndash;1 </sup>of BAP, and 0.1 mg&middot;l<sup>&ndash;1 </sup>of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg&middot;l<sup>&ndash;1 </sup>of IBA 70&ndash;75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides. &nbsp; &nbsp;

scholarly journals In Vitro Propagation of Aconitum chasmanthum Stapf Ex Holmes: An Endemic and Critically Endangered Plant Species of the Western Himalaya

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 586
Author(s):  
Shah Rafiq ◽  
Nasir Aziz Wagay ◽  
Irshad Ahmad Bhat ◽  
Zahoor Ahmad Kaloo ◽  
Sumaira Rashid ◽  
...  
Keyword(s):  
Plant Species ◽  
Shoot Regeneration ◽  
Large Scale ◽  
Basal Medium ◽  
Critically Endangered ◽  
Endangered Plant ◽  
Ms Medium ◽  
Endangered Plant Species ◽  
Chilling Treatment

Aconitum chasmanthum Stapf ex Holmes, a highly valued medicinal plant, is a critically endangered plant species with restricted global distribution. Because there is no published report on the in vitro micropropagation of A. chasmanthum, the present study was undertaken to contribute to the development of an efficient micropropagation protocol for its conservation. Seeds collected from the wild showed enhanced germination after being given a chilling treatment (−4 °C and −20 °C) for different durations (10, 20, 30 and 40 days). Seeds given a chilling treatment of −4 °C for 10 days showed enhanced germination rates of 47.59 ± 0.53% with a mean germination time of 10.78 ± 0.21 days compared to seeds kept at room temperature when grown in an MS basal medium. Nodes, leaves and stems, taken from 20–40-day-old seedlings, were used as an explant for micropropagation. An MS medium supplemented with different concentrations of cytokinins (BAP, Kn), auxins (2,4-D, NAA), and an additive adenine sulphate were tested for callusing, direct shoot regeneration and rooting. Only nodal explants responded and showed direct multiple shoot regeneration with 7 ± 0.36 shoots with an elongation of 5.51 ± 0.26 cm in the MS medium supplemented with BAP 0.5 mg/L, and with a response time (RT) of 10.41 ± 0.51 days and a percentage culture response of 77.77 ± 2.77%. Rhizome formation was observed after 8 weeks, with the highest culture response of 36.66 ± 3.33% in the MS basal media with an RT of 43.75 ± 0.50 days. These rhizomes showed a 60% germination rate within 2 weeks and developed into plantlets. The present in vitro regeneration protocol could be used for the large-scale propagation and conservation of A. chasmanthum.

scholarly journals Production of Δ7-Sterols from In Vitro Root Cultures of Endangered Gypsophila trichotoma

2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200
Author(s):  
Petranka Zdraveva ◽  
Pavlinka Popova ◽  
Aleksandar Shkondrov ◽  
Ilina Krasteva ◽  
Iliana Ionkova
Keyword(s):  
Plant Species ◽  
In Vitro Cultures ◽  
Endangered Plant ◽  
Root Cultures ◽  
Endangered Plant Species ◽  
Red Data Book ◽  
Aerial Parts ◽  
Data Book

Species from the genus Gypsophila are known for their medicinal, industrial and decorative applications. G. trichotoma Wend. is an endangered plant species for the Bulgarian flora according to the Red Data Book. Δ7-Sterols, which are unusual and rare in the plant kingdom, are present in the roots of this species. In previous studies different in vitro cultures were established from aerial parts of the species. The objective of this study was to explore the possibility for production of Δ7-sterols from in vitro cultured roots of G. trichotoma. The root cultures were grown on six modified MS media and the quantity of sterols was analyzed. These findings will serve to solve the important matter of the role of nutrients on sterols biosynthesis.

Site examination for threatened and endangered plant species

1982 ◽  
Vol 6 (4) ◽  
pp. 307-316 ◽  
Author(s):  
F. Glenn Goff ◽  
Gary A. Dawson ◽  
John J. Rochow
Keyword(s):  
Plant Species ◽  
Endangered Plant ◽  
Endangered Plant Species

scholarly journals Recovery of Globba wengeri (C.E.C. Fisch.) K.J. Williams, critically endangered plant species from Serchhip District in Mizoram, Northeast India

2020 ◽  
Vol 7 (2) ◽  
pp. 178-181
Author(s):  
Lalnun Mawia ◽  
Vanlalhruaii Ralte ◽  
H. Lalruatsanga ◽  
Zothan Mawia ◽  
P.C. Vanlalhluna ◽  
...  
Keyword(s):  
Life History ◽  
Plant Species ◽  
Natural Habitat ◽  
Northeast India ◽  
Critically Endangered ◽  
Endangered Plant ◽  
Endangered Plant Species ◽  
The Family ◽  
First Time

Globba wengeri (C.E.C. Fisch.) K.J. Williams, former state flower of Mizoram, a rare and critically endangered plant species, commonly known as ‘dancing girl’, belonging to the family Zingiberaceae, is reported in this communication for the first time from Serchhip District in Mizoram at an elevation of about 1187 m a.s.l. It was found on moist, watery and rocky slopes. The plant is under severe threat in the natural habitat and therefore, further studies are required to determine life history and particular survival threats of this species.

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