A high frequency of bulblet regeneration was achieved for the endemic and
endangered ornamental plant Muscari azureum using immature embryos. Immature
embryos of M. azureum were cultured on a callus induction medium consisting
of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1
L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus
clusters were transferred to a bulblet induction medium consisting of MS
mineral salts and vitamins containing different concentrations and
combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1
agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved
from immature embryos after 5-6 months of culture initiation. Well-developed
bulblets were excised and individually rooted on ? strength MS medium
supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and
6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>