Biochemical Studies on Sphingolipid of Artemia franciscana (I) Isolation and Characterization of Sphingomyelin

Lipids ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 635-643 ◽  
Author(s):  
Hisao Kojima ◽  
Takashi Inoue ◽  
Mutsumi Sugita ◽  
Saki Itonori ◽  
Masahiro Ito
Keyword(s):  
Artemia Franciscana ◽  
Isolation And Characterization ◽  
Biochemical Studies

Genetic and biochemical studies of asparagine-linked oligosaccharide assembly

1982 ◽  
Vol 300 (1099) ◽  
pp. 207-223 ◽  
Keyword(s):  
Protein Synthesis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Isolation And Characterization ◽  
Oligosaccharide Processing ◽  
Biochemical Studies ◽  
The Common ◽  
Specific Lipid ◽  
Temperature Sensitive Phenotype

The formation of N -glycosidic linkages of eukaryotic glycoproteins involves the assembly of a specific lipid-linked precursor oligosaccharide in the endoplasmic reticulum. This oligosaccharide is transferred from the lipid carrier to appropriate asparagine residues during protein synthesis. The protein-linked oligosaccharide then undergoes processing reactions that include both removal and addition of carbohydrate residues. In this paper we report recent studies from our laboratory on the synthesis of asparagine-linked oligosaccharides. In the first part we describe the isolation and characterization of temperature-sensitive mutants of yeast blocked at specific stages in the assembly of the lipid-linked oligosaccharide. In addition, we are using these mutants to clone the genes for the enzymes in this pathway by complementation of the temperature-sensitive phenotype. The second part deals with the topography of asparagine-linked oligosaccharide assembly. Our studies on the transmembrane movement of sugar residues during the assembly of secreted glycoproteins from cytoplasmic precursors are presented. Finally, experiments on the control of protein-linked oligosaccharide processing are described. Recent data are presented on the problem of how specific oligosaccharides are assembled from the common precursors at individual sites on glycoproteins.

scholarly journals Biochemical studies on the isolation and characterization of human spleen haemosiderin

1984 ◽  
Vol 223 (1) ◽  
pp. 31-38 ◽  
Author(s):  
M P Weir ◽  
J F Gibson ◽  
T J Peters
Keyword(s):  
Minor Component ◽  
Iron Oxyhydroxide ◽  
Human Spleen ◽  
Major Band ◽  
Isolation And Characterization ◽  
Biochemical Studies ◽  
Characteristic Group ◽  
A Minor ◽  
Proteolytic Product

Haemosiderin was isolated from thalassaemic human spleens by centrifugation through concentrated KI solutions. A method for solubilizing haemosiderin was developed which leaves the iron oxyhydroxide cores and constituent polypeptides intact, facilitating further purification and analysis. Purified haemosiderin contained no detectable haem, trace amounts of carbohydrate, and iron and phosphorus in a molar ration of 6:1; much of the phosphate may be present as core-adsorbed. Several lipids were present, but it is not certain whether these are contaminants or components of the haemosiderin granules. In all preparations examined, a characteristic group of six to seven peptides of apparent Mr 12 900-17 800 were found, with a major band at Mr 14 500 and, in addition, a minor component of Mr 42 000; these peptides co-chromatographed with the cores. Negatively stained electron micrographs suggest that these peptides form an incomplete shell about the cores, consistent with the view that haemosiderin is a proteolytic product of ferritin.

scholarly journals Biochemical studies on ricin. XIX. Isolation and characterization of two constituent polypeptide chains of ricin E.

1978 ◽  
Vol 42 (4) ◽  
pp. 851-859 ◽  
Author(s):  
Gunki FUNATSU ◽  
Takashi MISE ◽  
Hideki MATSUDA ◽  
Masaru FUNATSU
Keyword(s):  
Isolation And Characterization ◽  
Biochemical Studies ◽  
Polypeptide Chains

scholarly journals Biochemical studies on ricin. XVIII. Isolation and characterization of ricin E from castor beans.

1977 ◽  
Vol 41 (10) ◽  
pp. 2041-2046 ◽  
Author(s):  
Takashi MISE ◽  
Gunki FUNATSU ◽  
Masatsune ISHIGURO ◽  
Masaru FUNATSU
Keyword(s):  
Isolation And Characterization ◽  
Biochemical Studies

Isolation and characterization of vibriophage vB_Vc_SrVc9: an effective agent in preventing Vibrio campbellii infections in brine shrimp nauplii ( Artemia franciscana )

2020 ◽  
Author(s):  
C.O. Lomelí‐Ortega ◽  
A.J. Martínez‐Sández ◽  
D.R. Barajas‐Sandoval ◽  
A.G. Reyes ◽  
F. Magallón‐Barajas ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Artemia Franciscana ◽  
Effective Agent ◽  
Isolation And Characterization ◽  
Vibrio Campbellii

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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