Isolation and characterization of vibriophage vB_Vc_SrVc9: an effective agent in preventing Vibrio campbellii infections in brine shrimp nauplii ( Artemia franciscana )

2020 ◽  
Author(s):  
C.O. Lomelí‐Ortega ◽  
A.J. Martínez‐Sández ◽  
D.R. Barajas‐Sandoval ◽  
A.G. Reyes ◽  
F. Magallón‐Barajas ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Artemia Franciscana ◽  
Effective Agent ◽  
Isolation And Characterization ◽  
Vibrio Campbellii

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

Biochemical Studies on Sphingolipid of Artemia franciscana (I) Isolation and Characterization of Sphingomyelin

Lipids ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 635-643 ◽  
Author(s):  
Hisao Kojima ◽  
Takashi Inoue ◽  
Mutsumi Sugita ◽  
Saki Itonori ◽  
Masahiro Ito
Keyword(s):  
Artemia Franciscana ◽  
Isolation And Characterization ◽  
Biochemical Studies

scholarly journals Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test

2021 ◽  
Vol 10 (9) ◽  
pp. 207-215
Author(s):  
Yoan De Nanda Herru Adlis Santoni ◽  
Mai Efdi
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Hexane Fraction ◽  
Spectroscopic Methods ◽  
Brine Shrimp Lethality ◽  
Isolation And Characterization

Pometia pinnata leaves were extracted and fractionated using n-hexane, dichloromethane, ethyl acetate, and methanol. The four fractions obtained were screened for cytotoxic testing using the Brine shrimp lethality test (BSLT) method, n-hexane fraction has the highest LC50 419,855 mg/L.The n-hexane fraction was continued for the isolation stage and a secondary metabolite compound was obtained, namely brassicasterol. The structure of this secondary metabolites was determined using spectroscopic methods (UV-Vis, FTIR, and NMR).

scholarly journals Expression and characterization of the JAK kinase and STAT protein from brine shrimp, Artemia franciscana☆

2010 ◽  
Vol 28 (5-6) ◽  
pp. 774-782 ◽  
Author(s):  
Chia-Hsiung Cheng ◽  
Gen-Der Chen ◽  
Maw-Sheng Yeh ◽  
Cheng-Ying Chu ◽  
Ya-Li Hsu ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Artemia Franciscana ◽  
Jak Kinase

Cloning and Characterization of cDNAs Encoding Trehalase from Post-Dormant Embryos of the Brine Shrimp, Artemia franciscana

1999 ◽  
Vol 16 (2) ◽  
pp. 269-277 ◽  
Author(s):  
Shin Tanaka ◽  
Fumiko Nambu ◽  
Ziro Nambu
Keyword(s):  
Brine Shrimp ◽  
Artemia Franciscana

scholarly journals Isolation and Characterization of Infectious Vibrio Species from the Whiteleg Shrimp: Litopenaeus vannamei

2020 ◽  
Vol 2 (1) ◽  
pp. 36
Keyword(s):  
Litopenaeus Vannamei ◽  
Economic Loss ◽  
Vibrio Species ◽  
Shrimp Farming ◽  
Microbial Risk Assessment ◽  
Microbial Risk ◽  
Isolation And Characterization ◽  
Vibrio Campbellii ◽  
Vibrio Spp

Aquaculture shrimp farming has serious problems with diseases caused by viruses and bacteria, which lead to severe economic loss. The genus Vibrio is a group of facultative anaerobic microbes, most frequently found in aquatic environments and marine hosts. The Gram-negative genus Vibrio is one of the most important classes of bacterial pathogens in aquaculture systems and is a key cause of high mortality. The isolation, identification, and molecular characterization of Vibrio spp are studied from infected shrimp Litopenaeus vannamei. The microorganisms were isolated from the tissues of a white gut infected shrimp collected from diseased aquaculture ponds. After subculturing, microbial isolates were obtained using TCBS agar plates for vibrio selection, and the biochemical key was developed. Meanwhile, 11 isolates of bacteria belonging to the Vibrionaceae family have been identified in this study. For these, six Vibrio species-related isolates are responsible for vibriosis in the shrimp. These included Vibrio harveyi, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio owensii, Vibrio rotiferianus, and Vibrio alginolyticus. The extraction of DNA was carried out with a QIAamp DNA Mini Kit (QIAGEN). The extracted DNA was analyzed using an electrophoresis of 1 % agarose gel, the consistency and quantity of Vibrio spp's gDNA. NanoDropTM 2000 spectrophotometer (Thermo Scientific) was used for determination. In addition, the Agilent 2100 Bioanalyzer (Agilent Technologies) has been used to verify the gDNA libraries' validity, purity, concentration, and scale. In addition, Vibrio spp, a phylogenetic tree focused on maximum likelihood and bootstrapping, was performed using the MEGA version to examine the evolutionary phylogenetic relationship between the strains. This study's main objective would be useful in integrating strain variation in predicting microbiology and microbial risk assessment and may provide scientific guidelines for major Vibrio strains that may be responsible for vibriosis infection in the management of shrimp aquaculture disease.

Sign in / Sign up

Export Citation Format

Share Document