Protective effect of a vitamin E analog, phosphatidylchromanol, against oxidative hemolysis of human erythrocytes

The peroxidation of human erythrocytes induced by peroxyl radical initiator and its inhibition by several gallate esters (e.g., propyl, methyl, ethyl) and Trolox (a more polar analogue of vitamin E) have been studied. The antioxidant activity was determined on erythrocytes against hemolysis generated by a thermal activator, 2,2prime-azobis-(2-amidinopropane)dihydrogenchloride. It was found that propyl gallate and its two analogues were more effective than Trolox in preventing cell lysis. However, the combination of gallate esters and Trolox produced a protective effect exceeding the arithmetic sum of their individual contributions. These perceived synergisms occur at more than one level of Trolox at a given level of a gallate ester.Key words: Trolox, gallates, synergism, peroxyl radicals.

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Protective effect of some herbal medicine against oxidative hemolysis of human erythrocytes

Atherosclerosis ◽

10.1016/s0021-9150(00)81398-2 ◽

2000 ◽

Vol 151 (1) ◽

pp. 307

Author(s):

S. Asgary ◽

G.H. Naderi ◽

A. Alighanadi ◽

S. Golbon

Keyword(s):

Herbal Medicine ◽

Protective Effect ◽

Human Erythrocytes ◽

Oxidative Hemolysis

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Protective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytes

Food and Chemical Toxicology ◽

10.1016/j.fct.2009.03.017 ◽

2009 ◽

Vol 47 (6) ◽

pp. 1372-1377 ◽

Cited By ~ 59

Author(s):

Ana S. Magalhães ◽

Branca M. Silva ◽

José A. Pereira ◽

Paula B. Andrade ◽

Patrícia Valentão ◽

...

Keyword(s):

Protective Effect ◽

Human Erythrocytes ◽

Cydonia Oblonga ◽

Oxidative Hemolysis

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Protective effect of some herbal medicine against oxidative hemolysis of human erythrocytes

Journal of Molecular and Cellular Cardiology ◽

10.1016/s0022-2828(01)90019-7 ◽

2001 ◽

Vol 33 (6) ◽

pp. A5

Author(s):

Seddigheh Asgary ◽

Gholam-Ali Naderi ◽

Roya Kelishadi Ali-Reza ◽

Alighanadi Sima

Keyword(s):

Herbal Medicine ◽

Protective Effect ◽

Human Erythrocytes ◽

Oxidative Hemolysis

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Protective Effect of Vitamin E on Immune Triggered, Granulocyte Mediated Endothelial Injury

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661027 ◽

1984 ◽

Vol 51 (01) ◽

pp. 089-092 ◽

Cited By ~ 24

Author(s):

M A Boogaerts ◽

J Van de Broeck ◽

H Deckmyn ◽

C Roelant ◽

J Vermylen ◽

...

Keyword(s):

Vitamin E ◽

Protective Effect ◽

Umbilical Vein ◽

Endothelial Damage ◽

Endothelial Injury ◽

Mediated Effects ◽

Anti Oxidant ◽

Endothelial Cell Cultures ◽

Vitro Model

SummaryThe effect of alfa-tocopherol on the cell-cell interactions at the vessel wall were studied, using an in vitro model of human umbilical vein endothelial cell cultures (HUEC). Immune triggered granulocytes (PMN) will adhere to and damage HUEC and platelets enhance this PMN mediated endothelial injury. When HUEC are cultured in the presence of vitamin E, 51Cr-leakage induced by complement stimulated PMN is significantly decreased and the enhanced cytotoxicity by platelets is completely abolished (p <0.001).The inhibition of PMN induced endothelial injury is directly correlated to a diminished adherence of PMN to vitamin E- cultured HUEC (p <0.001), which may be mediated by an increase of both basal and stimulated endogenous prostacyclin (PGI2) from alfa-tocopherol-treated HUEC (p <0.025). The vitamin E-effect is abolished by incubation of HUEC with the irreversible cyclo-oxygenase inhibitor, acetylsalicylic acid, but the addition of exogenous PGI2 could not reproduce the vitamin E-mediated effects.We conclude that vitamin E exerts a protective effect on immune triggered endothelial damage, partly by increasing the endogenous anti-oxidant potential, partly by modulating intrinsic endothelial prostaglandin production. The failure to reproduce vitamin E-protection by exogenously added PGI2 may suggest additional, not yet elucidated vitamin E-effects on endothelial metabolism.

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