Expression of the cell death protein CED-4 of Caenorhabditis elegans in transgenic tobacco plants confers resistance to Meloidogyne incognita

2012 ◽  
Vol 6 (4) ◽  
pp. 275-284
Author(s):  
Abdellatif Bahaji ◽  
Thihan Padukkavidana ◽  
Robert T. Gaeta ◽  
Carlos Tristan ◽  
Glenda W. Polack ◽  
...  
Keyword(s):  
Cell Death ◽  
Caenorhabditis Elegans ◽  
Transgenic Tobacco ◽  
Meloidogyne Incognita ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cell Death Protein

scholarly journals Chitin Biosynthesis Inhibition of Meloidogyne incognita by RNAi-Mediated Gene Silencing Increases Resistance to Transgenic Tobacco Plants

2020 ◽  
Vol 21 (18) ◽  
pp. 6626
Author(s):  
Vimalraj Mani ◽  
Chinreddy Subramanyam Reddy ◽  
Seon-Kyeong Lee ◽  
Soyoung Park ◽  
Hyoung-Rai Ko ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Transgenic Tobacco ◽  
Meloidogyne Incognita ◽  
Chitin Synthase ◽  
Egg Mass ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Wide Range ◽  
Transgenic Lines ◽  
Plant Parasitic

Meloidogyne incognita is a devastating plant parasitic nematode that causes root knot disease in a wide range of plants. In the present study, we investigated host-induced RNA interference (RNAi) gene silencing of chitin biosynthesis pathway genes (chitin synthase, glucose-6-phosphate isomerase, and trehalase) in transgenic tobacco plants. To develop an RNAi vector, ubiquitin (UBQ1) promoter was directly cloned, and to generate an RNAi construct, expression of three genes was suppressed using the GATEWAY system. Further, transgenic Nicotiana benthamiana lines expressing dsRNA for chitin synthase (CS), glucose-6-phosphate isomerase (GPI), and trehalase 1 (TH1) were generated. Quantitative PCR analysis confirmed endogenous mRNA expression of root knot nematode (RKN) and revealed that all three genes were more highly expressed in the female stage than in eggs and in the parasitic stage. In vivo, transformed roots were challenged with M. incognita. The number of eggs and root knots were significantly decreased by 60–90% in RNAi transgenic lines. As evident, root galls obtained from transgenic RNAi lines exhibited 0.01- to 0.70-fold downregulation of transcript levels of targeted genes compared with galls isolated from control plants. Furthermore, phenotypic characteristics such as female size and width were also marginally altered, while effect of egg mass per egg number in RNAi transgenic lines was reduced. These results indicate the relevance and significance of targeting chitin biosynthesis genes during the nematode lifespan. Overall, our results suggest that further developments in RNAi efficiency in commercially valued crops can be applied to employ RNAi against other plant parasitic nematodes.

scholarly journals Inhibition of Phenolic Acid Metabolism Results in Precocious Cell Death and Altered Cell Morphology in Leaves of Transgenic Tobacco Plants

1998 ◽  
Vol 10 (11) ◽  
pp. 1801-1816 ◽  
Author(s):  
Lodovico Tamagnone ◽  
Angel Merida ◽  
Nicola Stacey ◽  
Kitty Plaskitt ◽  
Adrian Parr ◽  
...  
Keyword(s):  
Cell Death ◽  
Transgenic Tobacco ◽  
Cell Morphology ◽  
Phenolic Acid ◽  
Acid Metabolism ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Altered Cell

Inhibition of Phenolic Acid Metabolism Results in Precocious Cell Death and Altered Cell Morphology in Leaves of Transgenic Tobacco Plants

1998 ◽  
Vol 10 (11) ◽  
pp. 1801 ◽  
Author(s):  
Lodovico Tamagnone ◽  
Angel Merida ◽  
Nicola Stacey ◽  
Kitty Plaskitt ◽  
Adrian Parr ◽  
...  
Keyword(s):  
Cell Death ◽  
Transgenic Tobacco ◽  
Cell Morphology ◽  
Phenolic Acid ◽  
Acid Metabolism ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Altered Cell

scholarly journals Induction of Cell Death in Transgenic Plants Expressing a Fungal Glucose Oxidase

1998 ◽  
Vol 11 (6) ◽  
pp. 555-562 ◽  
Author(s):  
Kemal Kazan ◽  
Fiona R. Murray ◽  
Ken C. Goulter ◽  
Danny J. Llewellyn ◽  
John M. Manners
Keyword(s):  
Cell Death ◽  
Salicylic Acid ◽  
Transgenic Plants ◽  
Glucose Oxidase ◽  
Transgenic Tobacco ◽  
Plant Cell ◽  
Gene Induction ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Defense Gene

Hydrogen peroxide (H2O2) has been implicated in the induction of plant defense genes and programmed cell death. Expression of a chimeric fungal glucose oxidase (GO) gene driven by a pathogen- and wound-inducible promoter was evaluated in transgenic tobacco and canola as a possible tool for engineering plant cell death and defense gene induction. Expression of this gene under the control of a peroxidase gene promoter resulted in the accumulation of relatively low levels of H2O2 in the young leaves of transgenic tobacco plants and this was not sufficient to cause any visible cell death and defense gene induction as measured by PR-1a mRNA induction. Older leaves of transgenic tobacco plants, however, exhibited visible necrotic lesions and constitutively expressed PR-1a mRNA when grown under high light conditions. Inoculation of cotyledons of control and transgenic canola with Leptosphaeria maculans resulted in rapid cotyledon senescence in the transgenic plants. Strong activators of the peroxidase promoter, i.e., wounding and inoculation of transgenic plants with Cercospora nicotianae, were not sufficient to trigger any additional visible cell death in transgenic tobacco plants, compared with controls. However, when exogenous glucose was supplied to transgenic tissue, massive cell death and PR-1a gene induction were observed in tobacco. Exogenously applied salicylic acid further increased the rate and extent of cell death. Our results suggest that efficacy of GO expression for the induction of cell death is restricted by glucose supply in the plants and are consistent with a role for salicylic acid in the potentiation of plant cell death by H2O2.

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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