Breeding of the transgenic chrysanthemum (Chrysanthemum morifolium Ramat.) carrying aphid-resistance gene, Pinellia ternata agglutinin (PTA)

2020 ◽  
Vol 14 (3) ◽  
pp. 255-262
Author(s):  
SongHui Pak ◽  
MyongHun Han ◽  
HyonU Li ◽  
HyongBom Pak ◽  
JongNam Li
Keyword(s):  
Resistance Gene ◽  
Chrysanthemum Morifolium ◽  
Aphid Resistance ◽  
Pinellia Ternata ◽  
Transgenic Chrysanthemum ◽  
Chrysanthemum Morifolium Ramat

scholarly journals Morphological and Physiological Changes in Transgenic Chrysanthemum morifolium Ramat. ‘Ogura-nishiki’ with rolC

2006 ◽  
Vol 75 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Takashi Kubo ◽  
Masato Tsuro ◽  
Atsushi Tsukimori ◽  
Yoshiaki Shizukawa ◽  
Tetsuyuki Takemoto ◽  
...  
Keyword(s):  
Chrysanthemum Morifolium ◽  
Physiological Changes ◽  
Transgenic Chrysanthemum ◽  
Chrysanthemum Morifolium Ramat

scholarly journals Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media

2021 ◽  
Vol 9 (2) ◽  
pp. 109-115
Author(s):  
Ahmad Saifun Naser ◽  
Muhammad Wisnu
Keyword(s):  
Tissue Culture ◽  
Callus Formation ◽  
Leaf Explants ◽  
Chrysanthemum Morifolium ◽  
Stem Explants ◽  
Appearance Time ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
Chrysanthemum Morifolium Ramat

Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.

Sign in / Sign up

Export Citation Format

Share Document