scholarly journals Comparative Study of Molecular Approaches for the Detection of Influenza Virus from Patient Samples Using Real-time PCR: Prospective Disease Burden Study in Kerala (India) from 2010 to 2016

2018 ◽  
Vol 20 (8) ◽  
Author(s):  
Seetha Dayakar ◽  
Heera R. Pillai ◽  
Vineetha P. Thulasi ◽  
Devakikutty Jayalekshmi ◽  
Radhakrishnan R. Nair
Keyword(s):  
Influenza Virus ◽  
Comparative Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Disease Burden ◽  
Molecular Approaches

COMPARATIVE STUDY OF SPECIFICITY AND SENSITIVITY FOR IHHNV DETECTION BETWEEN REAL-TIME PCR AND LAMP METHODS

2010 ◽  
Vol 36 (5) ◽  
pp. 984-989
Author(s):  
Zhe ZHAO ◽  
Chun-Hua REN ◽  
Xiao JIANG ◽  
Lü-Ping ZHANG ◽  
Juan FENG ◽  
...  
Keyword(s):  
Comparative Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Specificity And Sensitivity

scholarly journals Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiyu Zhang ◽  
Ming Yao ◽  
Zhihui Tang ◽  
Daning Xu ◽  
Yan Luo ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Standard Deviation ◽  
Real Time ◽  
Real Time Pcr ◽  
Virus Isolation ◽  
Simultaneous Detection ◽  
Conventional Pcr ◽  
Pcr Assay ◽  
Duck Tembusu Virus ◽  
Relative Standard

Abstract Background Pathogens including duck-origin avian influenza virus (AIV), duck-origin Newcastle disease virus (NDV) and duck Tembusu virus (DTMUV) posed great harm to ducks and caused great economic losses to the duck industry. In this study, we aim to develop a triplex real-time polymerase chain reaction (PCR) assay to detect these three viruses as early as possible in the suspicious duck flocks. Results The detection limit of the triplex real-time PCR for AIV, NDV, and DTMUV was 1 × 101 copies/μL, which was at least 10 times higher than the conventional PCR. In addition, the triplex assay was highly specific, and won’t cross-react with other duck pathogens. Besides, the intra-day relative standard deviation and inter-day relative standard deviation were lower than 4.44% for these viruses at three different concentrations. Finally, a total of 120 clinical samples were evaluated by the triplex real-time PCR, the conventional PCR and virus isolation, and the positive rates for these three methods were 20.83, 21.67, 19.17%, respectively. Taking virus isolation as the gold standard, the diagnostic specificity and positive predictive value of the three viruses were all above 85%, while the diagnostic sensitivity and negative predictive value of the three viruses were all 100%. Conclusion The developed triplex real-time PCR is fast, specific and sensitive, and is feasible and effective for the simultaneous detection of AIV, NDV, and DTMUV in ducks.

A comparative study of DNA extraction methods to be used in real-time PCR based quantification of ochratoxin A-producing molds in food products

Food Control ◽  
2012 ◽  
Vol 25 (2) ◽  
pp. 666-672 ◽  
Author(s):  
Alicia Rodríguez ◽  
Mar Rodríguez ◽  
M. Isabel Luque ◽  
Annemarie F. Justesen ◽  
Juan J. Córdoba
Keyword(s):  
Comparative Study ◽  
Real Time ◽  
Ochratoxin A ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Food Products ◽  
Extraction Methods ◽  
Dna Extraction Methods

Detection of Equine-2 Influenza Virus by the Real-Time PCR

2003 ◽  
Vol 14 (4) ◽  
pp. 111-117 ◽  
Author(s):  
Shigeo SUGITA ◽  
Tomio MATSUMURA
Keyword(s):  
Influenza Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
The Real

scholarly journals Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent

2009 ◽  
Vol 162 (1-2) ◽  
pp. 88-90 ◽  
Author(s):  
Andrew D. Sails ◽  
David Saunders ◽  
Stephanie Airs ◽  
David Roberts ◽  
Gary Eltringham ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Influenza Virus A ◽  
Syncytial Virus ◽  
Specific Reagent

A Comparative Study of Six Trichinella Species by Real-time PCR Assay

2018 ◽  
Author(s):  
Valeria Dilcheva ◽  
Ivelin Vladov ◽  
Svetlozara Petkova
Keyword(s):  
Comparative Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay
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