Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation

2012 ◽  
Vol 166 (6) ◽  
pp. 1480-1490 ◽  
Author(s):  
Yan Zhao ◽  
Wei Sun ◽  
Ying Wang ◽  
Praveen K. Saxena ◽  
Chun-Zhao Liu
Keyword(s):  
Forced Ventilation ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Mass Multiplication ◽  
Rhodiola Crenulata

Daminozide Enhances Vigor and Steviol Glycoside Yield of Stevia (Stevia rebaudiana Bert.) Propagated in the Temporary Immersion Bioreactor

2022 ◽  
Author(s):  
Rizka Tamania Saptari ◽  
Rizkita Rachmi Esyanti ◽  
Riza Arief Putranto
Keyword(s):  
Stevia Rebaudiana ◽  
Feedback Regulation ◽  
Optimum Concentration ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Kaurenoic Acid ◽  
Biosynthesis Gene ◽  
Inhibitor Treatment ◽  
Ga Biosynthesis

Abstract Stevia (Stevia rebaudiana Bertoni) contains sweet compound widely used as natural sweetener, steviol glycoside (SG). SG is a diterpenoid secondary metabolite synthesized from ent-kaurenoic acid, the same precursor of Gibberellin (GA). Therefore, in this study, a GA inhibitor, Daminozide (0, 10, 20 ppm) was used to block ent-kaurenoic acid conversion towards GA synthesis in attempt to increase SG content of stevia propagated in Temporary Immersion Bioreactor (TIB). Daminozide in 10 mg/L was observed to be the optimum concentration which increased biomass weight and SG content (stevioside and rebaudioside A) up to 40%. The treatment also increased transcripts accumulation of genes enrolled in SG biosynthesis, such as SrKA13H, SrUGT85C2, and SrUGT76G1, indicating SG pathway become more active due to the inhibition of GA pathway. Furthermore, the inhibition of GA was also indicated by the upregulated expression of GA biosynthesis gene (GA3ox) as the result of feedback regulation, and the downregulated expression of GA catabolism gene (GA2ox2) as the result of feed-forward regulation caused by inhibitor treatment.

scholarly journals Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Maria Lopes Martins Avelar ◽  
Sérgio Bruno Fernandes ◽  
Eduardo Oliveira Silva ◽  
Vinícius Politi Duarte ◽  
...  
Keyword(s):  
Eucalyptus Urophylla ◽  
Spectral Quality ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Multiplication

Efficient production of virus-free apple plantlets using the temporary immersion bioreactor system

2020 ◽  
Vol 61 (4) ◽  
pp. 779-785
Author(s):  
Na-Young Kim ◽  
Ho-Dong Hwang ◽  
Jin-Ho Kim ◽  
Bo-Min Kwon ◽  
Daeil Kim ◽  
...  
Keyword(s):  
Efficient Production ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System
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