Effect of activated charcoal on multiplication of African yam (Dioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor (RITA®)

2013 ◽  
Vol 50 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Frederik Polzin ◽  
Isabelle Sylvestre ◽  
Eveline Déchamp ◽  
Pascal Ilbert ◽  
Hervé Etienne ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Nodal Segments ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Dioscorea Cayenensis

scholarly journals An Efficient Method of Pennisetum x Advena ‘Rubrum’ Seedlings Production Using Temporary Immersion Bioreactor and Agar Cultures

2021 ◽  
Author(s):  
Mariusz Pożoga ◽  
Dawid Olewnicki ◽  
Elżbieta Wójcik-Gront
Keyword(s):  
Liquid Medium ◽  
Efficient Method ◽  
Activated Charcoal ◽  
Agar Medium ◽  
Ms Medium ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bright Green ◽  
Bioreactor System

Abstract The aim of this study was to propose an efficient method of Pennisetum x advena ‘Rubrum’ micropropagation. Agar cultures with MS medium supplemented with BAP in various concentrations (0.5 mg/L-2 mg/L) and a temporary immersion bioreactor system (TIS) with liquid medium MS with an addition of 1 mg/L BAP were used. For rooting ½ MS medium with different auxin combinations (IBA, NAA) and activated charcoal was utilized. The most efficient method turned out to be TIS which produced 36.9 new plants in four weeks. The seedlings were slender in shape, bright green in colour with no signs of hyperhydricity. The most suitable agar medium produced 19.5 new plants in an eight week period. Rooting should be carried on ½ MS supplemented with 0.5 mg/L IBA and 0.5 mg/L NAA with an 84% rooting rate. The addition of activated charcoal inhibited rooting.

scholarly journals Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1093-1098 ◽  
Author(s):  
Jericó J. Bello-Bello ◽  
Adriana Canto-Flick ◽  
Eduardo Balam-Uc ◽  
Eunice Gómez-Uc ◽  
Manuel L. Robert ◽  
...  
Keyword(s):  
Shoot Elongation ◽  
Nodal Segments ◽  
High Survival ◽  
Shoot Induction ◽  
Capsicum Chinense ◽  
Temporary Immersion Bioreactor ◽  
In Vitro Proliferation ◽  
Temporary Immersion ◽  
Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

scholarly journals Micropropagation of pineapple (Ananas comosus L. Var. Smooth cayenne) in temporary immersion bioreactor system (TIPS)

2021 ◽  
Vol 12 (2) ◽  
pp. 207-209
Author(s):  
L.A. Sani ◽  
I.S. Usman ◽  
A.U. Nasir ◽  
M.M. Abdulmalik
Keyword(s):  
Activated Charcoal ◽  
Ananas Comosus ◽  
Multiplication Rate ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
The Family ◽  
Root Morphogenesis ◽  
Bioreactor System ◽  
Planting Materials

Pineapple is an important edible fruit in the family Bromeliaceae popularly grown in the tropical and subtropical countries. Commercial prodution of pineapple requires large volume of planting materials which could not easily be obtained using conventional method of propagation. A protocol for mass propagation of pineapple (Ananas comosus L. var.smooth cayenne) using temporary immersion bioreactor system has been developed.The protocol involves four immersion cycles in Murashinge and Skoog (MS) media fortified with 1mg/L or 2mg/L 6-Benzylaminopurine (BAP) with or without 0.25g/L activated charcoal (AC). The highest multiplication rate (120 -130 plants/bottle) was obtained when media was fortified with 1mg/l or 2mg/L BAP alone. The presence of activated charcoal (AC) promoted root morphogenesis, resulting in significant increase in roots formation in BAP suplemented media. A combination of BAP with AC significantly increased the number of competent plants(20 – 30 plants/bottle) after four weeks of culture in temporary immersion system. The system is recommended for rapid and efficient micropropagation of pineapple.

Cannabis sativa L. Micropropagation in Temporary Immersion Bioreactor System

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
H Lata ◽  
S Chandra ◽  
IA Khan ◽  
MA ElSohly
Keyword(s):  
Cannabis Sativa ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System

Daminozide Enhances Vigor and Steviol Glycoside Yield of Stevia (Stevia rebaudiana Bert.) Propagated in the Temporary Immersion Bioreactor

2022 ◽  
Author(s):  
Rizka Tamania Saptari ◽  
Rizkita Rachmi Esyanti ◽  
Riza Arief Putranto
Keyword(s):  
Stevia Rebaudiana ◽  
Feedback Regulation ◽  
Optimum Concentration ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Kaurenoic Acid ◽  
Biosynthesis Gene ◽  
Inhibitor Treatment ◽  
Ga Biosynthesis

Abstract Stevia (Stevia rebaudiana Bertoni) contains sweet compound widely used as natural sweetener, steviol glycoside (SG). SG is a diterpenoid secondary metabolite synthesized from ent-kaurenoic acid, the same precursor of Gibberellin (GA). Therefore, in this study, a GA inhibitor, Daminozide (0, 10, 20 ppm) was used to block ent-kaurenoic acid conversion towards GA synthesis in attempt to increase SG content of stevia propagated in Temporary Immersion Bioreactor (TIB). Daminozide in 10 mg/L was observed to be the optimum concentration which increased biomass weight and SG content (stevioside and rebaudioside A) up to 40%. The treatment also increased transcripts accumulation of genes enrolled in SG biosynthesis, such as SrKA13H, SrUGT85C2, and SrUGT76G1, indicating SG pathway become more active due to the inhibition of GA pathway. Furthermore, the inhibition of GA was also indicated by the upregulated expression of GA biosynthesis gene (GA3ox) as the result of feedback regulation, and the downregulated expression of GA catabolism gene (GA2ox2) as the result of feed-forward regulation caused by inhibitor treatment.

MASS SHOOT PROLIFERATION OF PISTACIA KHINJUK STOCKS USING TEMPORARY IMMERSION BIOREACTOR SYSTEM (TIS)

2014 ◽  
pp. 145-151 ◽  
Author(s):  
E. Tilkat ◽  
V. Süzerer ◽  
Y. Ersali ◽  
A. Hoșer ◽  
F.M. Kilinç ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Pistacia Khinjuk ◽  
Bioreactor System

scholarly journals Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Maria Lopes Martins Avelar ◽  
Sérgio Bruno Fernandes ◽  
Eduardo Oliveira Silva ◽  
Vinícius Politi Duarte ◽  
...  
Keyword(s):  
Eucalyptus Urophylla ◽  
Spectral Quality ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Multiplication
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