Attainment of 15-Fold Higher Affinity of a Fusarium-specific Single-Chain Antibody by Directed Molecular Evolution Coupled to Phage Display

2011 ◽  
Vol 52 (2) ◽  
pp. 111-122 ◽  
Author(s):  
Jin-Long Liu ◽  
Zu-Quan Hu ◽  
Shu Xing ◽  
Sheng Xue ◽  
He-Ping Li ◽  
...  
Keyword(s):  
Molecular Evolution ◽  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Directed Molecular Evolution

Isolation by phage display and characterization of a single-chain antibody specific for O6-methyldeoxyguanosine

2001 ◽  
Vol 46 (12) ◽  
pp. 1024-1029 ◽  
Author(s):  
Heping Dai ◽  
Rouhong Zhao ◽  
Haug Brenda ◽  
Sean M. Hemmingsen ◽  
Wei Xiao
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody

scholarly journals Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein

2007 ◽  
Vol 14 (6) ◽  
pp. 726-731 ◽  
Author(s):  
So-Hee Kim ◽  
Seung-Young Hwang ◽  
Yong-Seok Lee ◽  
In-Hak Choi ◽  
Sae-Gwang Park ◽  
...  
Keyword(s):  
Phage Display ◽  
Plasmodium Vivax ◽  
Binding Protein ◽  
Antibody Fragment ◽  
Neutralizing Antibody ◽  
Active Immunization ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Duffy Binding Protein ◽  
Erythrocyte Binding

ABSTRACT Phage display of single-chain variable fragment (scFv) antibodies is a powerful tool for selecting important, useful, and specific human antibodies. We constructed a library from three patients infected with Plasmodium vivax. Panning on recombinant PvRII enriched a population of scFvs that recognized region II of the P. vivax Duffy binding protein (DBP). Three clones of scFvs that reacted with PvRII were selected, and their biological functions were analyzed. These scFvs inhibited erythrocyte binding to DBP. Clone SFDBII92 had the greatest affinity (dissociation constant = 3.62 × 10−8 M) and the greatest inhibition activity (50% inhibitory concentration ≈ 2.9 μg/ml) to DBP. Thus, we demonstrated that human neutralizing antibody could be made from malaria patients using phage display and that these neutralizing scFvs should prove valuable for developing both passive and active immunization strategies based on DBP.

Characterization of a unique human single-chain antibody isolated by phage-display selection on membrane-bound mosquito midgut antigens

2002 ◽  
Vol 261 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Brian D Foy ◽  
Gerry F Killeen ◽  
Ross H Frohn ◽  
Daniel Impoinvil ◽  
Andrew Williams ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Mosquito Midgut ◽  
Midgut Antigens ◽  
Membrane Bound

Hevein-specific recombinant IgE antibodies from human single-chain antibody phage display libraries

2003 ◽  
Vol 278 (1-2) ◽  
pp. 271-281 ◽  
Author(s):  
Marja-Leena Laukkanen ◽  
Soili Mäkinen-Kiljunen ◽  
Kirsi Isoherranen ◽  
Tari Haahtela ◽  
Hans Söderlund ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Ige Antibodies ◽  
Phage Display Libraries ◽  
Antibody Phage ◽  
Antibody Phage Display

scholarly journals Protein III-based single-chain antibody phage display using bacterial cells bearing an additional genome of a gene-III-lacking helper phage

BioTechniques ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 760-765 ◽  
Author(s):  
Bizhi Shi ◽  
Huamao Wang ◽  
Shengrong Guo ◽  
Yuhong Xu ◽  
Zonghai Li ◽  
...  
Keyword(s):  
Phage Display ◽  
Bacterial Cells ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Helper Phage ◽  
Antibody Phage ◽  
Antibody Phage Display
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