Use of Synthetic Genes for Cloning, Production and Functional Expression of the Bacteriocins Enterocin A and Bacteriocin E 50-52 by Pichia pastoris and Kluyveromyces lactis

2014 ◽  
Vol 56 (6) ◽  
pp. 571-583 ◽  
Author(s):  
Juan J. Jiménez ◽  
Juan Borrero ◽  
Loreto Gútiez ◽  
Sara Arbulu ◽  
Carmen Herranz ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Kluyveromyces Lactis ◽  
Functional Expression ◽  
Synthetic Genes ◽  
Enterocin A

scholarly journals Cloning, Production, and Functional Expression of the Bacteriocin Enterocin A, Produced by Enterococcus faecium T136, by the Yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans

2012 ◽  
Vol 78 (16) ◽  
pp. 5956-5961 ◽  
Author(s):  
Juan Borrero ◽  
Gotthard Kunze ◽  
Juan J. Jiménez ◽  
Erik Böer ◽  
Loreto Gútiez ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enterococcus Faecium ◽  
Kluyveromyces Lactis ◽  
Hansenula Polymorpha ◽  
Functional Expression ◽  
Antimicrobial Activities ◽  
Arxula Adeninivorans ◽  
Content Type ◽  
Enterocin A

ABSTRACTThe bacteriocin enterocin A (EntA) produced byEnterococcus faeciumT136 has been successfully cloned and produced by the yeastsPichia pastorisX-33EA,Kluyveromyces lactisGG799EA,Hansenula polymorphaKL8-1EA, andArxula adeninivoransG1212EA. Moreover,P. pastorisX-33EA andK. lactisGG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities than the EntA produced byE. faeciumT136.

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115

2019 ◽  
Vol 122 ◽  
pp. 55-63 ◽  
Author(s):  
Huahua He ◽  
Chao Zhai ◽  
Meng Mei ◽  
Yi Rao ◽  
Yao Liu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Interferon Α ◽  
Pastoris Gs115 ◽  
Pichia Pastoris Gs115

Cloning, functional expression and characterization of Aspergillus sulphureus β-mannanase in Pichia pastoris

2007 ◽  
Vol 128 (3) ◽  
pp. 452-461 ◽  
Author(s):  
Xiaoling Chen ◽  
Yunhe Cao ◽  
Yuhua Ding ◽  
Wenqing Lu ◽  
Defa Li
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Aspergillus Sulphureus

scholarly journals Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris

2003 ◽  
Vol 376 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Isabel SOARES-SILVA ◽  
Dorit SCHULLER ◽  
Raquel P. ANDRADE ◽  
Fátima BALTAZAR ◽  
Fernanda CÁSSIO ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Pichia Pastoris ◽  
Plasma Membranes ◽  
Membrane Vesicle ◽  
Dry Weight ◽  
Functional Expression ◽  
Acid Uptake ◽  
Methanol Induction

In Saccharomyces cerevisiae the activity for the lactate–proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol·s−1·mg of dry weight−1. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. pastoris methanol-induced cells with Escherichia coli liposomes containing cytochrome c oxidase, as proton-motive force. These assays in reconstituted heterologous P. pastoris membrane vesicles demonstrate that S. cerevisiae Jen1p is a functional lactate transporter. Moreover, a S. cerevisiae strain deleted in the JEN1 gene was transformed with a centromeric plasmid containing JEN1 under the control of the glyceraldehyde-3-phosphate dehydrogenase constitutive promotor. Constitutive JEN1 expression and lactic acid uptake were observed in cells grown on either glucose and/or acetic acid. The highest Vmax (0.84 nmol·s−1·mg of dry weight−1) was obtained in acetic acid-grown cells. Thus overexpression of the S. cerevisiae JEN1 gene in both S. cerevisiae and P. pastoris cells resulted in increased activity of lactate transport when compared with the data previously reported in lactic acid-grown cells of native S. cerevisiae strains. Jen1p is the only S. cerevisiae secondary porter characterized so far by heterologous expression in P. pastoris at both the cell and the membrane-vesicle levels.

Functional Expression of a Thermostable Endoglucanase from Thermoascus aurantiacus RCKK in Pichia pastoris X-33 and Its Characterization

2018 ◽  
Vol 60 (10) ◽  
pp. 736-748 ◽  
Author(s):  
Kavish Kumar Jain ◽  
Sandeep Kumar ◽  
Kailash N. Bhardwaj ◽  
Ramesh Chander Kuhad
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Thermoascus Aurantiacus
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