Method for Label-Free Quantitative Proteomics for Sorghum bicolor L. Moench

Abstract Background Spotted stem borer- Chilo partellus - a Lepidopteran insect pest of Sorghum bicolor is responsible for major economic losses. It is an oligophagous pest, which bores through the plant stem, causing ‘deadheart’ and hampering the development of the main cob. We applied a label-free quantitative proteomics approach on three genotypes of S. bicolor with differential resistance/ susceptibility to insect pests, intending to identify the S. bicolor’s systemic protein complement contributing to C. partellus tolerance. Methods The proteomes of S. bicolor with variable resistance to insect pests, ICSV700, IS2205 (resistant) and Swarna (susceptible) were investigated and compared using label-free quantitative proteomics to identify putative leaf proteins contributing to resistance to C. partellus. Results The multivariate analysis on a total of 967 proteins led to the identification of proteins correlating with insect resistance/susceptibility of S. bicolor. Upon C. partellus infestation S. bicolor responded by suppression of protein and amino acid biosynthesis, and induction of proteins involved in maintaining photosynthesis and responding to stresses. The gene ontology analysis revealed that C. partellus-responsive proteins in resistant S. bicolor genotypes were mainly involved in stress and defense, small molecule biosynthesis, amino acid metabolism, catalytic and translation regulation activities. At steady-state, the resistant S. bicolor genotypes displayed at least two-fold higher numbers of unique proteins than the susceptible genotype Swarna, mostly involved in catalytic activities. Gene expression analysis of selected candidates was performed on S. bicolor by artificial induction to mimic C. partellus infestation. Conclusion The collection of identified proteins differentially expressed in resistant S. bicolor, are interesting candidates for further elucidation of their role in defense against insect pests.

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Use of Azospirillum irakense as bio fertilizer for sorghum (Sorghum bicolor L.)

Basrah Journal of Agricultural Sciences ◽

10.33762/bagrs.2012.54757 ◽

2012 ◽

Vol 25 (1) ◽

pp. 139-148

Author(s):

Thaher A. Taha

Keyword(s):

Sorghum Bicolor ◽

Sorghum Bicolor L

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Drought Resistance Mechanism and Adaptation to Water Stress in Sorghum [Sorghum bicolor (L.) Moench]

International Journal of Bio-resource and Stress Management ◽

10.23910/ijbsm/2018.9.1.3c0472 ◽

2018 ◽

Vol 9 (1) ◽

pp. 167-172

Author(s):

Rajani Verma ◽

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Ramesh Kumar ◽

Dr. Anamika Nath ◽

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...

Keyword(s):

Water Stress ◽

Sorghum Bicolor ◽

Drought Resistance ◽

Resistance Mechanism ◽

Sorghum Bicolor L

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Consórcio palma-sorgo sob lâminas de irrigação: balanço de água no solo e coeficientes da cultura

Agrometeoros ◽

10.31062/agrom.v27i2.26448 ◽

2020 ◽

Vol 27 (2) ◽

Author(s):

Cleber Pereira Alves ◽

Thieres George Freire Silva ◽

Hygor Kristoph Muniz Nunes Alves ◽

Alexandre Maniçoba da Rosa Ferraz Jardim ◽

Luciana Sandra Bastos de Souza ◽

...

Keyword(s):

Sorghum Bicolor ◽

Sorghum Bicolor L

Objetivou-se neste estudo quantificar a evapotranspiração real (ETr) e máxima da cultura (ETc) e os coeficientes da cultura (Kc) do consórcio palma-sorgo. O experimento foi conduzido no município de Serra Talhada, PE. O delineamento usado foi em blocos ao acaso, envolvendo cinco lâminas de irrigação (0, 25, 50, 75 e 100% da evapotranspiração de referência - ETo), sob sistema de cultivo consorciado palma-sorgo. O clone de palma forrageira utilizado foi a Orelha de Elefante Mexicana (Opuntia stricta (Haw.) Haw.) e o cultivar de sorgo, Sorghum bicolor (L.) Moench, a SF 15. O sorgo foi conduzido durante dois ciclos (planta e rebrota) compreendidos em um ciclo anual da palma. A quantificação da ETr e da ETc foi realizada através do resíduo do balanço de água no solo (BAS) a cada 14 dias, com a mensuração dos componentes hidrodinâmicos. As determinações da ETc e do Kc foram realizadas com base na lâmina de 75% da ETo. Os componentes do BAS foram submetidos à análise de regressão, sendo testados modelos polinomiais. Com exceção da variação do armazenamento de água no solo, os demais componentes hidrodinâmicos do solo cultivado sob sistema consorciado palma-sorgo respondem linearmente ao aumento de lâminas de irrigação. A evapotranspiração média diária do consórcio palma-sorgo é igual a 3,0 mm dia-1, independentemente da lâmina de irrigação. Os coeficientes do consórcio palma-sorgo são iguais a 0,40, 0,68, 0,90 e 0,52 durante as fases I, II, III e IV de emissão de cladódios.

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Performance and within-hybrid variability of three-way and single-crosses in grain sorghum, Sorghum bicolor (L) Moench

10.31274/rtd-180813-943 ◽

1971 ◽

Author(s):

Edward Joseph Walsh

Keyword(s):

Sorghum Bicolor ◽

Grain Sorghum ◽

Sorghum Bicolor L

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Effet antioxydant et antihyperglycémiant du seigle (Secale cereale L.) et du sorgho (Sorghum bicolor L.) chez le rat Wistar rendu diabétique

Phytothérapie ◽

10.3166/phyto-2018-0013 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S273-S283

Author(s):

Z. Mami-Soualem ◽

N. Brixi ◽

C. Beghdad ◽

M. Belarbi

Keyword(s):

Sorghum Bicolor ◽

Secale Cereale ◽

Stress Oxydant ◽

Habitudes Alimentaires ◽

Diabète Sucré ◽

Secale Cereale L ◽

Vitamine C ◽

Fibres Alimentaires ◽

Peroxydation Lipidique ◽

Sorghum Bicolor L

Le changement des habitudes alimentaires au cours de ces dernières années a abouti à l’apparition des maladies dites de civilisation, parmi elles le diabète sucré. Des approches diétothérapeutiques privilégient la consommation des produits céréaliers sous la forme la plus complète possible. Ce travail a pour but de tester l’efficacité des régimes expérimentaux préparés à base des grains complets des deux céréales, le sorgho (Sorghum bicolor L.) et le seigle (Secale cereale L.), sur la correction de l’hyperglycémie et des marqueurs du stress oxydant associés au diabète chez le rat mâle Wistar. Les résultats montrent que les rats diabétiques, soumis au régime seigle pendant quatre semaines, présentent une diminution significative de la glycémie qui atteint les 53,95 % à la fin de l’expérimentation, ce qui fait que leur taux en glycémie se rapproche de celui des normoglycémiques. Le sorgho blanc présente, d’une part, une diminution significative de la peroxydation lipidique intracellulaire et, d’autre part, une augmentation de l’activité de la glutathion peroxydase, la glutathion réductase ainsi que la vitamine C. Ces approches méthodologiques peuvent, en améliorant la connaissance de l’importance des céréales (fibres alimentaires solubles et des polyphénols) dans l’évolution du diabète, aboutir à des recommandations et à une éducation nutritionnelle du diabétique.

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