scholarly journals Application of Polymerase Chain Reaction to Detect HIV-1 DNA in Pools of Dried Blood Spots

2011 ◽  
Vol 51 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Vemu Lakshmi ◽  
Talasila Sudha ◽  
Dandona Rakhi ◽  
G. Anilkumar ◽  
Lalit Dandona
Keyword(s):  
Polymerase Chain Reaction ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Blood Spots ◽  
Polymerase Chain ◽  
Hiv 1

scholarly journals Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Bhavna H Chohan ◽  
Sandra Emery ◽  
Dalton Wamalwa ◽  
Grace John-Stewart ◽  
Maxwel Majiwa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Round Polymerase Chain Reaction ◽  
Blood Spots ◽  
Polymerase Chain ◽  
African Setting ◽  
Hiv 1

Single step detection of HIV-1 proviral DNA and housekeeping β-actin gene from dried blood spots by a monoplex polymerase chain reaction

2013 ◽  
Vol 187 (1) ◽  
pp. 203-206 ◽  
Author(s):  
Ipsita Choudhary ◽  
Vaishali Chimanpure ◽  
Ajit Patil ◽  
Robin Mukhopadhyaya ◽  
Ramesh Paranjape ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dried Blood Spots ◽  
Single Step ◽  
Actin Gene ◽  
Chain Reaction ◽  
Step Detection ◽  
Proviral Dna ◽  
Blood Spots ◽  
Polymerase Chain ◽  
Hiv 1

scholarly journals Erratum to: Application of Polymerase Chain Reaction to Detect HIV-1 DNA in Pools of Dried Blood Spots

2011 ◽  
Vol 51 (4) ◽  
pp. 536-536
Author(s):  
Vemu Lakshmi ◽  
Talasila Sudha ◽  
Rakhi Dandona ◽  
G. Anil Kumar ◽  
Lalit Dandona
Keyword(s):  
Polymerase Chain Reaction ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Blood Spots ◽  
Polymerase Chain ◽  
Hiv 1

Two Novel Nonradioactive Polymerase Chain Reaction-Based Assays of Dried Blood Spots, Genomic DNA, or Whole Cells for Fast, Reliable Detection of Z and S Mutations in the α1-Antitrypsin Gene

1992 ◽  
Vol 38 (10) ◽  
pp. 2100-2107 ◽  
Author(s):  
B S Andresen ◽  
I Knudsen ◽  
P K Jensen ◽  
K Rasmussen ◽  
N Gregersen
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Whole Cells ◽  
Blood Spots ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Alpha 1 Antitrypsin

Abstract Two new nonradioactive polymerase chain reaction (PCR)-based assays for the Z and S mutations in the alpha 1-antitrypsin gene are presented. The assays take advantage of PCR-mediated mutagenesis, creating new diagnostic restriction enzyme sites for unambiguous discrimination between test samples from individuals who are normal, heterozygous, or homozygous for the mutations. We show that the two assays can be performed with purified genomic DNA as well as with boiled blood spots. The new assays were validated by parallel testing with a technique in which PCR is combined with allele-specific oligonucleotide (ASO) probes. In all cases tested the results obtained by the different techniques were in accordance. The new assays can be used for prenatal diagnostics and can be performed directly with boiled tissue samples. Because the new assays are easy to perform and reliable, we conclude that they are well suited for routine diagnosis.

scholarly journals Detection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers

1996 ◽  
Vol 90 (5) ◽  
pp. 519-521 ◽  
Author(s):  
Balbir Singh ◽  
Janet Cox-Singh ◽  
Andy Olivier Miller ◽  
Mohammad Shukri Abdullah ◽  
Georges Snounou ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Spots ◽  
Reaction Amplification ◽  
Polymerase Chain ◽  
Filter Papers

scholarly journals Detection of Pathogens of Acute Febrile Illness Using Polymerase Chain Reaction from Dried Blood Spots

2021 ◽  
Author(s):  
Brian Grundy ◽  
Ursula Panzner ◽  
Jie Liu ◽  
Hyon Jin Jeon ◽  
Justin Im ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Whole Blood ◽  
Quantitative Polymerase Chain Reaction ◽  
Dried Blood Spots ◽  
Disease Diagnosis ◽  
Chain Reaction ◽  
Direct Pcr ◽  
Blood Spots ◽  
Wide Range ◽  
Polymerase Chain

Quantitative polymerase chain reaction (qPCR) of dried blood spots (DBS) for pathogen detection is a potentially convenient method for infectious disease diagnosis. This study tested 115 DBS samples paired with whole blood specimens of children and adolescent from Burkina Faso, Sudan, and Madagascar by qPCR for a wide range of pathogens, including protozoans, helminths, fungi, bacteria, and viruses. Plasmodium spp. was consistently detected from DBS but yielded a mean cycle threshold (Ct) 5.72 ± 1.6 higher than that from whole blood samples. A DBS qPCR Ct cutoff of 27 yielded 94.1% sensitivity and 95.1% specificity against the whole blood qPCR cutoff of 21 that has been previously suggested for malaria diagnosis. For other pathogens investigated, DBS testing yielded a sensitivity of only 8.5% but a specificity of 98.6% compared with whole blood qPCR. In sum, direct PCR of DBS had reasonable performance for Plasmodium but requires further investigation for the other pathogens assessed in this study.

Polymerase chain reaction amplification from dried blood spots on Guthrie cards

The Lancet ◽  
1990 ◽  
Vol 336 (8715) ◽  
pp. 639-640 ◽  
Author(s):  
E.I. Schwartz ◽  
S.E. Khalchitsky ◽  
R.C. Eisensmith ◽  
S.L.C. Woo
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Blood Spots ◽  
Reaction Amplification ◽  
Polymerase Chain ◽  
Guthrie Cards
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