scholarly journals Culture Media Statistical Optimization for Biomass Production of a Ligninolytic Fungus for Future Rice Straw Degradation

2013 ◽  
Vol 53 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Viviana Sarria-Alfonso ◽  
John Sánchez-Sierra ◽  
Mauricio Aguirre-Morales ◽  
Ivonne Gutiérrez-Rojas ◽  
Nubia Moreno-Sarmiento ◽  
...  
2019 ◽  
Vol 17 (2) ◽  
pp. 196
Author(s):  
Eliseo Amado-González ◽  
Alveiro Álvarez Ovallos ◽  
Alfonso Quijano Parra

Low frecuency electromagnetic fields effect (EMF) on growth cycles of yeast Saccharomyces cerevisiae wine strains Rv1 and Rhône were studied.  A cylindrical coil induced magnetic fields with inductions up to 0,39 mT. Exposure time to EMF varied between (1 – 10) min at 30 °C.  The biomass growth were monitored in the reactor culture media (yeast extract + by measurement optical density from (0 to 32) h. The biomass was found by dry weight. After yeast expose to the different EMF, the number of growth cycles decreased from 4 cycles to 2 or 1. However, the biomass production increased almost 50 %.  The best biomass production was found at 0.39 mT and 10 min exposure time.  Keywords: Electromagnetic fields, Saccharomyces cerevisiae, biomass production, RV1


2010 ◽  
Vol 56 (2) ◽  
pp. 128-137 ◽  
Author(s):  
D. Spadaro ◽  
A. Ciavorella ◽  
Z. Dianpeng ◽  
A. Garibaldi ◽  
M. L. Gullino

Few strains of Metschnikowia pulcherrima (Pitt) M.W. Miller are under development for control of postharvest pathogens on fruit. A substrate was developed to optimize the biomass production of M. pulcherrima strain BIO126. Different complex nutrient sources, with or without pH control, were tested. Growth in yeast extract provided at concentrations ≥30 g·L–1yielded the highest biomass. The addition of two carbon sources, d-mannitol and l-sorbose, at 5 g·L–1each, significantly improved yeast growth. The greatest amount of yeast growth occurred when pH values of the medium ranged from 5.0 to 7.5. A combination of yeast extract, d-mannitol, and l-sorbose (YEMS), probably with diauxic utilization, showed a synergistic effect, widening the exponential phase (maximum specific growth rate of 0.45 h–1) and increasing the final cell number (1.5 × 109cells·mL–1) and dry biomass (6.0 g·L–1) in well-controlled batch fermentation. In efficacy trials on ‘Golden Delicious’ apples, M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively). Also on ‘Gala’ apples, the best reduction of grey and blue mould incidence was obtained with cells grown in YEMS (58.1% and 50.5%, respectively).


2017 ◽  
Vol 40 (12) ◽  
pp. 1733-1742 ◽  
Author(s):  
Francisco J. Choix ◽  
Elena Polster ◽  
Rosa Isela Corona-González ◽  
Raúl Snell-Castro ◽  
Hugo O. Méndez-Acosta

2017 ◽  
Vol 120 ◽  
pp. 6-14 ◽  
Author(s):  
Mauricio J. Alessandrello ◽  
María S. Juárez Tomás ◽  
Paula Isaac ◽  
Diana L. Vullo ◽  
Marcela A. Ferrero

2020 ◽  
Vol 5 (2) ◽  
pp. 51-69
Author(s):  
Raul Enrique Martínez-Herrera ◽  
◽  
Yessica Nallely Juárez-Ibarra ◽  
Verónica Almaguer- Cantú ◽  
María Elizabeth Alemán-Huerta

Author(s):  
Suwapha Sawiphak ◽  
Aroon Wongjiratthiti ◽  
Chanankarn Saengprasan

Dioscorea alata (purple yam) is a tuber crop that contains plenty of nutrients. It is widely cultivated in Thailand, but it is underutilized. In this study, the suitability of purple yam to replace potato dextrose media for fungal growth was investigated. Mushrooms and molds were grown on purple yam dextrose agar (PYDA), whereas yeasts were cultured in purple yam dextrose broth (PYDB). Response surface methodology (RSM) with Box-Behnken design (BBD) was used to optimize the culture conditions for Saccharomyces cerevisiae biomass production. The growth profile of S. cerevisiae in PYDB under optimized culture conditions was also studied. All test mushrooms and molds recorded the highest colony diameter and mycelial dry weight on PYDA containing 40% purple yam. Similar to mushrooms and molds, yeasts in PYDB with 40% purple yam showed the highest number of cells. The growth of fungi on purple yam dextrose media was significantly higher than those on potato dextrose media under standard conditions. The optimal conditions from the RSM results for the biomass production of S. cerevisiae in PYDB were purple yam concentration of 49.61%, dextrose concentration of 4.87%, pH value of 5.74, and inoculum size of 7.00%. The biomass of S. cerevisiae in PYDB under the optimal conditions obtained from the results of the optimization by RSM was thirty times higher than S. cerevisiae biomass in potato dextrose broth under standard conditions. Our results suggest that purple yam could be an alternative to potato dextrose media for fungal cultivation.


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