Comparison of Dilute Acid and Sulfite Pretreatment for Enzymatic Saccharification of Earlywood and Latewood of Douglas fir

2013 ◽  
Vol 7 (1) ◽  
pp. 362-370 ◽  
Author(s):  
Chao Zhang ◽  
Xiaochun Lei ◽  
C. Tim Scott ◽  
J.Y. Zhu ◽  
Kecheng Li
Keyword(s):  
Enzymatic Saccharification ◽  
Douglas Fir ◽  
Dilute Acid

scholarly journals Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification

2010 ◽  
Vol 101 (13) ◽  
pp. 4900-4906 ◽  
Author(s):  
Chenlin Li ◽  
Bernhard Knierim ◽  
Chithra Manisseri ◽  
Rohit Arora ◽  
Henrik V. Scheller ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Saccharification ◽  
Dilute Acid ◽  
Biomass Recalcitrance ◽  
Ionic Liquid Pretreatment

Comparison of ultrasound-assisted Fenton reaction and dilute acid-catalysed steam explosion pretreatment of corncobs: cellulose characteristics and enzymatic saccharification

RSC Advances ◽  
2016 ◽  
Vol 6 (80) ◽  
pp. 76848-76854 ◽  
Author(s):  
Sujun Wang ◽  
Xianhong Ouyang ◽  
Wenya Wang ◽  
Qipeng Yuan ◽  
Aixia Yan
Keyword(s):  
Steam Explosion ◽  
Fenton Reaction ◽  
Enzymatic Saccharification ◽  
Dilute Acid ◽  
Steam Explosion Pretreatment ◽  
Ultrasound Assisted

As an emerging method for lignocellulose pretreatment, the ultrasound-assisted Fenton reaction is not well developed in comparison to the dilute acid-catalysed steam explosion.

Enzymatic Saccharification and Fermentation of Xylose-Optimized Dilute Acid–Treated Lignocellulosics

2005 ◽  
Vol 124 (1-3) ◽  
pp. 0947-0962 ◽  
Author(s):  
Yun-Chin Chung ◽  
Alan Bakalinsky ◽  
Michael H. Penner
Keyword(s):  
Enzymatic Saccharification ◽  
Dilute Acid

Xylanase supplementation on enzymatic saccharification of dilute acid pretreated poplars at different severities

Cellulose ◽  
2013 ◽  
Vol 20 (4) ◽  
pp. 1937-1946 ◽  
Author(s):  
Chao Zhang ◽  
Xinshu Zhuang ◽  
Zhao Jiang Wang ◽  
Fred Matt ◽  
Franz St. John ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Dilute Acid

scholarly journals Enhanced Enzymatic Saccharification of Mixed Sawdust Wastes: Comparison of SPORL, Dilute Acid, Formic Acid, and Ethanol Organosolv Pretreatments

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Yuan Liang ◽  
Yanbo Yin ◽  
Haifeng Zhou ◽  
Yuanyu Tian
Keyword(s):  
Formic Acid ◽  
Enzymatic Saccharification ◽  
Fiber Surface ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Hemicellulose Removal ◽  
Ethanol Pretreatment ◽  
Hydrolysis Of

Abstract. Utilization of the huge quantity of sawdust wastes is urgent. In this study, SPORL, dilute acid pretreatment (DA), formic acid pretreatment (FA), ethanol pretreatment (EtOH/H2O), and sulfuric acid catalyzed ethanol pretreatment (EtOH/H2O/H2SO4), on improving enzymatic hydrolysis of mixed sawdust wastes were comprehensively compared. EtOH/H2O/H2SO4 was the most effective pretreatment in lignin removal from sawdust fiber cell wall, while FA was much more effective in hemicellulose removal. After the pretreatments, the crystallinity of cellulose increased because of the removal of amorphous hemicellulose and lignin. Moreover, the fiber surface became coarse and porous, especially after EtOH/H2O/H2SO4, the structure was destroyed into fragments, which enhanced the cellulase accessibility of cellulose. Therefore, the glucose yield of EtOH/H2O/H2SO4 pretreated substrate was highest among these five pretreatments, achieved at 91.4% with a cellulase loading of only 10 FPU/g glucan.   Resumen. Es urgente aprovechar la gran cantidad de residuos de aserrín. En este estudio, SPORL, pretratamiento con ácido diluido (DA), pretratamiento con ácido fórmico (FA), pretratamiento con etanol (EtOH/H2O) y pretratamiento con etanol catalizado con ácido sulfúrico (EtOH/H2O/H2SO4), sobre la mejora de la hidrólisis enzimática de residuos de aserrín mezclado fueron comparados de manera integral. EtOH/H2O/H2SO4 fue el pretratamiento más eficaz para eliminar la lignina de la pared celular de la fibra de aserrín, mientras que el FA fue mucho más eficaz para eliminar la hemicelulosa. Después de los pretratamientos, la cristalinidad de la celulosa aumentó debido a la eliminación de hemicelulosa amorfa y lignina. Además, la superficie de la fibra se volvió gruesa y porosa, especialmente después de EtOH/H2O/H2SO4, la estructura se destruyó en fragmentos, lo que mejoró la accesibilidad de celulasa de la celulosa. Por lo tanto, el rendimiento de glucosa del sustrato pretratado con EtOH/H2O/H2SO4 fue el más alto entre estos cinco pretratamientos, alcanzado al 91,4% con una carga de celulasa de solo 10 FPU / g de glucano.

scholarly journals Valorisation of bambara and cowpea haulms for bioethanol production

2020 ◽  
Author(s):  
◽  
Somiame Itseme Okuofu
Keyword(s):  
Ethanol Yield ◽  
Promising Result ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Enzymatic Saccharification ◽  
Deep Eutectic Solvents ◽  
Sugar Yield ◽  
Dilute Acid Pretreatment ◽  
Bioethanol Production ◽  
Dilute Acid

Bambara and cowpea are important pulses grown in semi-arid South Africa due to their balanced nutrient profile and drought resilient capacity. The haulm is the lignocellulosic residue obtained after grain harvest and are rich in carbohydrates. However, these haulms are underutilised and under researched. The aim of the study, therefore, was to investigate the potential to valorise bambara haulms (BGH) and cowpea haulms (CH) to bioethanol which is the most promising biofuel with commercial prospects currently. The structural and chemical composition of BGH and CH was elucidated using techniques such as compositional analysis, XRD, FTIR, ICP-AES, and SEM. Results indicated a volatile matter and fixed carbon mass fraction of 77.70% and 13.15% (w/w) in BGH and 76.16% and 16.26% (w/w) in CH respectively. The polysaccharides make up the largest fraction (51%), followed by extractives (> 20%), while the lignin in BGH (12%) and CH (10%) was low. X-ray diffraction pattern showed a higher percentage of amorphous regions in BGH (78%) than CH (56%). CH was then subjected to dilute acid pretreatment (DAP) to enhance biosugar production for bioethanol fermentation. The effects of operational factors for DAP including temperature, time, and acid concentration on sugar yield and inhibitor formation was investigated and optimised using response surface methodology (RSM). The solid recovered after DAP was subjected to prehydrolysis with simultaneous saccharification and fermentation (PSSF). In addition, the pretreatment hydrolysate was detoxified and fermented to ethanol using cocultures of Saccharomyces cerevisiae BY4743 and Scheffersomyces stipitis wild type (PsY633). A total ethanol titre of 15.67 g/L was obtained corresponding to 75% conversion efficiency. On the other hand, BGH was subjected to deep eutectic solvent (DES) pretreatment. Five deep eutectic solvents were prepared and screened for their effectiveness in improving enzymatic sugar yield. This was achieved by pretreating BGH with each DES followed by a 48 h enzymatic saccharification. Choline chloride – lactic acid (ChCl-LA) treatment provided the most promising result and was further optimised by investigating the effect of different temperatures and time on cellulose loss and enzymatic sugar yield. ChCl-LA pretreatment at 100°C for 1 h was observed to be the best condition for maximum sugar recovery. The hydrolysate thus obtained was concentrated and fermented for 72 h with S. cerevisiae BY4743. A maximum ethanol yield of 11.57 g/L was obtained. From the results, it is evident that bambara and cowpea haulm are promising substrates for bioethanol production. Dilute acid hydrolysis was shown to be effective in the pretreatment of CH with over 85% of the theoretical sugar recoverable for conversion to bioethanol. In addition, deep eutectic solvents are effective media for breaking the recalcitrance in BGH to achieve high sugar yield for conversion to bioethanol. However, further studies are required to reduce cellulose loss during pretreatment to improve bioethanol yield.

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