Efficacy and Metagenomic Analysis of the Stabilized Anaerobic Lignocellulolytic Microbial Consortium from Bubalus bubalis Rumen with Rice Straw Enrichment for Methane Production

2020 ◽  
Author(s):  
Nitiya Thongbunrod ◽  
Pawinee Chaiprasert
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Microbial Consortium ◽  
Bubalus Bubalis ◽  
Metagenomic Analysis

Efficient Lignin Decomposing Microbial Consortium to Hasten Rice-Straw Composting with Moderate GHGs Fluxes

2021 ◽  
Author(s):  
Pradeep Kumar Dash ◽  
Soumya Ranjan Padhy ◽  
Pratap Bhattacharyya ◽  
Ankita Pattanayak ◽  
Soumya Routray ◽  
...  
Keyword(s):  
Rice Straw ◽  
Microbial Consortium

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

Electrohydrolysis pretreatment methods to enhance the methane production from anaerobic digestion of rice straw using graphite electrode

2019 ◽  
Vol 142 ◽  
pp. 1-10 ◽  
Author(s):  
Jyoti Kainthola ◽  
Mohd Shariq ◽  
Ajay S. Kalamdhad ◽  
Vaibhav V. Goud
Keyword(s):  
Anaerobic Digestion ◽  
Rice Straw ◽  
Methane Production ◽  
Graphite Electrode

Enhancing methane production from rice straw by extrusion pretreatment

2014 ◽  
Vol 122 ◽  
pp. 34-41 ◽  
Author(s):  
Xiaohua Chen ◽  
YaLei Zhang ◽  
Yu Gu ◽  
Zhanguang Liu ◽  
Zheng Shen ◽  
...  
Keyword(s):  
Rice Straw ◽  
Methane Production

Use of Lignocellulolytic Microbial Consortium and pH Amendment on Composting Efficacy of Rice Straw

2013 ◽  
Vol 21 (3-4) ◽  
pp. 121-133 ◽  
Author(s):  
H. Kausar ◽  
Mohd Razi Ismail ◽  
Halimi Mohd Saud ◽  
Radziah Othman ◽  
S. Habib
Keyword(s):  
Rice Straw ◽  
Microbial Consortium

scholarly journals Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Jingjing Wan ◽  
Yuhang Jing ◽  
Yue Rao ◽  
Shicheng Zhang ◽  
Gang Luo
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Quantitative Pcr ◽  
Methane Production ◽  
Bacterial Pathogens ◽  
Methane Yield ◽  
Metagenomic Analysis ◽  
Waste Activated Sludge ◽  
Hydrogen Yield

ABSTRACT Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H 2 /g volatile solids [VS]) and methane yield (150.7 ml CH 4 /g VS) in the TM process were higher than those (6.7 ml H 2 /g VS and 127.8 ml CH 4 /g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH 4 /g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that different microbial community compositions were established in the hydrogen reactors of the TM and MM processes, which also significantly changed the microbial community compositions in the following methane reactors compared to that with the M process. The dynamics of bacterial pathogens were also evaluated. For the TM process, the reduced diversity and total abundance of bacterial pathogens in WAS were observed in the hydrogen reactor and were further reduced in the methane reactor, as revealed by metagenomic analysis. The results also showed not all bacterial pathogens were reduced in the reactors. For example, Collinsella aerofaciens was enriched in the hydrogen reactor, which was also confirmed by quantitative PCR (qPCR) analysis. The study further showed that qPCR was more sensitive for detecting bacterial pathogens than metagenomic analysis. Although there were some differences in the relative abundances of bacterial pathogens calculated by metagenomic and qPCR approaches, both approaches demonstrated that the TM process was more efficient for the removal of bacterial pathogens than the MM and M processes. IMPORTANCE This study developed an efficient process for bioenergy (H 2 and CH 4 ) production from WAS and elucidates the dynamics of bacterial pathogens in the process, which is important for the utilization and safe application of WAS. The study also made an attempt to combine metagenomic and qPCR analyses to reveal the dynamics of bacterial pathogens in anaerobic processes, which could overcome the limitations of each method and provide new insights regarding bacterial pathogens in environmental samples.

Effects of isolated fungal pretreatment on bio-methane production through the co-digestion of rice straw and buffalo dung

Energy ◽  
2020 ◽  
Vol 206 ◽  
pp. 118107
Author(s):  
Altaf Alam Noonari ◽  
Rasool Bux Mahar ◽  
Abdul Razaque Sahito ◽  
Khan Muhammad Brohi
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Fungal Pretreatment ◽  
Buffalo Dung

scholarly journals Degradation of rice straw at low temperature using a novel microbial consortium LTF-27 with efficient ability

2020 ◽  
Vol 304 ◽  
pp. 123064 ◽  
Author(s):  
Guoxiang Zheng ◽  
Ting Yin ◽  
Zhaoxin Lu ◽  
Stopira yannick benz Boboua ◽  
Jiachen Li ◽  
...  
Keyword(s):  
Low Temperature ◽  
Rice Straw ◽  
Microbial Consortium

Enhanced methane production from anaerobic digestion of rice straw pretreated by Fe3+/CaO2 catalyzed Fenton-like process

2020 ◽  
Vol 11 ◽  
pp. 100472
Author(s):  
Weiwei Huang ◽  
Fei Yang ◽  
Wenli Huang ◽  
Zhongfang Lei ◽  
Zhenya Zhang
Keyword(s):  
Anaerobic Digestion ◽  
Rice Straw ◽  
Methane Production

Enhancing ethanol and methane production from rice straw by pretreatment with liquid waste from biogas plant

2018 ◽  
Vol 178 ◽  
pp. 290-298 ◽  
Author(s):  
Forough Momayez ◽  
Keikhosro Karimi ◽  
Ilona Sárvári Horváth
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Liquid Waste ◽  
Biogas Plant
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