Application of First Order Derivative UV Spectrophotometry Coupled with H-Point Standard Addition to the Simultaneous Determination of Melamine and Dicyandiamide in Milk

Zileuton is an orally active inhibitor of 5-lipoxygenase and found to inhibit leukotrienes (LTB4 , LTC4, LTD4 , and LTE4) formation and well-known for the effective treatment of asthma. Rapid, economical and simple ‘Zero order UV-spectrophotometry’ and ‘First order derivative UV-spectrophotometry’ have been developed for estimation zileuton in bulk and in-house tablets. Zileuton showed maximum absorbance at 258.4 nm in 0.01M NaOH. For Zero order UV-spectrophotometric method (Method I), two wavelengths 243.4 nm and 271.0 nm were selected for the determination of area under curve (AUC). In First order derivative UV-spectrophotometry (Method II), two wavelengths 260.0 nm and 279.60 nm were selected for determination of AUC. For both these methods, zileuton followed linearity in the concentration range of 4 - 24 µg/ mL with correlation coefficient value of 0.999. Both methods were validated as per ICH guidelines.

Download Full-text

Development of first-order Derivative Spectrophotometry for Simultaneous Determination of Domperidone and Ilaprazole

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.01.258 ◽

2020 ◽

Vol 12 (01) ◽

Keyword(s):

Simultaneous Determination ◽

Derivative Spectrophotometry ◽

Order Derivative ◽

First Order

Download Full-text

Estimation of Tadalafil Using Derivative Spectrophotometry in Bulk Material and in Pharmaceutical Formulation

International Journal of Spectroscopy ◽

10.1155/2014/392421 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Zamir G. Khan ◽

Amod S. Patil ◽

Atul A. Shirkhedkar

Keyword(s):

Wavelength Range ◽

Area Under Curve ◽

Pharmaceutical Formulation ◽

Second Order ◽

Order Derivative ◽

Uv Spectrophotometry ◽

First Order ◽

Beer's Law ◽

Beer’S Law ◽

Derivative Uv Spectrophotometry

Four simple, rapid, accurate, precise, reliable, and economical UV-spectrophotometric methods have been proposed for the determination of tadalafil in bulk and in pharmaceutical formulation. “Method A” is first order derivative UV spectrophotometry using amplitude, “method B” is first order derivative UV spectrophotometry using area under curve technique, “method C” is second order derivative UV spectrophotometry using amplitude, and “method D” is second order derivative UV spectrophotometry using area under curve technique. The developed methods have shown best results in terms of linearity, accuracy, precision, and LOD and LOQ for bulk drug and marketed formulation as well. In N,N-dimethylformamide, tadalafil showed maximum absorbance at 284 nm. For “method A” amplitude was recorded at 297 nm while for “method B” area under curve was integrated in the wavelength range of 290.60–304.40 nm. For “method C” amplitude was measured at 284 nm while for “method D” area under curve was selected in the wavelength range of 280.80–286.20 nm. For methods A and B, tadalafil obeyed Lambert-Beer’s law in the range of 05–50 μg/mL while for “methods C and D”, tadalafil obeyed Lambert-Beer’s law in the range of 20–70 μg/mL, and-for “methods A, B, C, and D” the correlation coefficients were found to be > than 0.999.

Download Full-text

UV Spectrophotometric Simultaneous Determination of Paracetamol and Ibuprofen in Combined Tablets by Derivative and Wavelet Transforms

The Scientific World JOURNAL ◽

10.1155/2014/313609 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 4

Author(s):

Vu Dang Hoang ◽

Dong Thi Ha Ly ◽

Nguyen Huu Tho ◽

Hue Minh Thi Nguyen

Keyword(s):

Simultaneous Determination ◽

Wavelet Transforms ◽

Hybrid Approach ◽

Uv Spectra ◽

Order Derivative ◽

First Order ◽

Spectrophotometric Methods ◽

Combined Use ◽

Linear Concentration

The application of first-order derivative and wavelet transforms to UV spectra and ratio spectra was proposed for the simultaneous determination of ibuprofen and paracetamol in their combined tablets. A new hybrid approach on the combined use of first-order derivative and wavelet transforms to spectra was also discussed. In this application, DWT (sym6 and haar), CWT (mexh), and FWT were optimized to give the highest spectral recoveries. Calibration graphs in the linear concentration ranges of ibuprofen (12–32 mg/L) and paracetamol (20–40 mg/L) were obtained by measuring the amplitudes of the transformed signals. Our proposed spectrophotometric methods were statistically compared to HPLC in terms of precision and accuracy.

Download Full-text