APPLICATION OF UV-SPECTROPHOTOMETRY FOR DETERMINATION OF ZILEUTON IN BULK AND IN TABLETS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (06) ◽  
pp. 40-44
Author(s):  
S. B. Ganorkar ◽  
◽  
D. M Dhumal ◽  
A. A Shirkhedkar
Keyword(s):  
Simple Zero ◽  
Zero Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
Active Inhibitor ◽  
First Order ◽  
Ich Guidelines ◽  
Orally Active ◽  
Derivative Uv Spectrophotometry

Zileuton is an orally active inhibitor of 5-lipoxygenase and found to inhibit leukotrienes (LTB4 , LTC4, LTD4 , and LTE4) formation and well-known for the effective treatment of asthma. Rapid, economical and simple ‘Zero order UV-spectrophotometry’ and ‘First order derivative UV-spectrophotometry’ have been developed for estimation zileuton in bulk and in-house tablets. Zileuton showed maximum absorbance at 258.4 nm in 0.01M NaOH. For Zero order UV-spectrophotometric method (Method I), two wavelengths 243.4 nm and 271.0 nm were selected for the determination of area under curve (AUC). In First order derivative UV-spectrophotometry (Method II), two wavelengths 260.0 nm and 279.60 nm were selected for determination of AUC. For both these methods, zileuton followed linearity in the concentration range of 4 - 24 µg/ mL with correlation coefficient value of 0.999. Both methods were validated as per ICH guidelines.

NOVEL ZERO ORDER UV-SPECTROPHOTOMETRIC AND FIRST ORDER DERIVATIVE UV-AUC METHODS FOR ESTIMATION OF ETOMIDATE IMPARTING HYDROTROPIC SOLUBLIZATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 15-20
Author(s):  
Atul A. Shirkhedkar ◽  
◽  
Saurabh B. Ganorkar
Keyword(s):  
Water Solubility ◽  
Pharmaceutical Formulations ◽  
Zero Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Poor Water Solubility ◽  
Hydrotropic Agent

Simple, novel UV-spectrophotometric methods have been developed and validated for the estimation of etomidate imparting a hydrotropic solublization phenomenon. Etomidate is an imidazole derivative, approved as a short acting intravenous anesthetic having poor water solubility. As the UV-spectrophotometric estimation was not seen in any published literature, we have reported it here; zero order UV-spectrophotometry (Method I) and First Order Derivative UV-spectrophotometry (Method II) with the application of hydrotropy to counteract the poor water solubility of etomidate. The present research endeavour utilizes propylene glycol (PG) (2% v/v with distilled water) as the hydrotropic agent for the UV-spectrophotometric determination of etomidate. The drug was found to be stable in solution form with the aforementioned hydrotropic agent. Method I was carried out by measuring absorbance at λmax of 243.40 nm whilst for Method II, a uniform area between 245.40 - 268.40 nm was measured and plotted against concentration. The linearity range was obeyed to be in between 4 - 24 μg/mL with a correlation coefficient of 0.999 for both the developed methods. The non - interference of a hydrotropic agent ensures the specificity for proposed methods. The mean recovery of 99.60 (Method I) and 100.28 (Method II) reflects accuracy for developed methods. The method precision results were found to be within limit; expressed in % RSD; no more than 2.0%. The developed UV-spectrophotometric methods for the estimation of etomidate are simple, less time consuming and eco-friendly. The developed methods were validated according to ICH guidelines and proved statistically accurate, precise and sensitive, which can be extended to a routine determination of etomidate in bulk and pharmaceutical formulations.

scholarly journals Estimation of Tadalafil Using Derivative Spectrophotometry in Bulk Material and in Pharmaceutical Formulation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Zamir G. Khan ◽  
Amod S. Patil ◽  
Atul A. Shirkhedkar
Keyword(s):  
Wavelength Range ◽  
Area Under Curve ◽  
Pharmaceutical Formulation ◽  
Second Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
First Order ◽  
Beer's Law ◽  
Beer’S Law ◽  
Derivative Uv Spectrophotometry

Four simple, rapid, accurate, precise, reliable, and economical UV-spectrophotometric methods have been proposed for the determination of tadalafil in bulk and in pharmaceutical formulation. “Method A” is first order derivative UV spectrophotometry using amplitude, “method B” is first order derivative UV spectrophotometry using area under curve technique, “method C” is second order derivative UV spectrophotometry using amplitude, and “method D” is second order derivative UV spectrophotometry using area under curve technique. The developed methods have shown best results in terms of linearity, accuracy, precision, and LOD and LOQ for bulk drug and marketed formulation as well. In N,N-dimethylformamide, tadalafil showed maximum absorbance at 284 nm. For “method A” amplitude was recorded at 297 nm while for “method B” area under curve was integrated in the wavelength range of 290.60–304.40 nm. For “method C” amplitude was measured at 284 nm while for “method D” area under curve was selected in the wavelength range of 280.80–286.20 nm. For methods A and B, tadalafil obeyed Lambert-Beer’s law in the range of 05–50 μg/mL while for “methods C and D”, tadalafil obeyed Lambert-Beer’s law in the range of 20–70 μg/mL, and-for “methods A, B, C, and D” the correlation coefficients were found to be > than 0.999.

scholarly journals Spectrophotometric Determination of Olmutinib in Bulk by Area under Curve and First Order Derivative Methods and its Validation as per ICH guidelines

2019 ◽  
Vol 9 (4-A) ◽  
pp. 349-354
Author(s):  
BALU KHANDARE ◽  
Atish C. Musle ◽  
Sanket S. Arole ◽  
Pravin V. Popalghat
Keyword(s):  
Area Under Curve ◽  
Limit Of Detection ◽  
Order Derivative ◽  
Spectrometric Method ◽  
First Order ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Limit Of Quantitation

Abstract: A simple, precise and economical UV-spectrophotometric method has been developed for the estimation of Olmutinib from bulk. Two methods were developed First method (A) applied was area under curve (AUC) in which the area was integrated in wavelength from 262-272nm. Second method (B) was first order derivative spectrometric method. In this method absorbance at λmin=256.57nm, λmax=282.83nm and zero cross=267.68nm was measured. Calibration curves were plotted for the method by using instrumental response at selected wavelength and concentration of analyte in the solution. In both the methods, linearity was observed in the concentration range of 2-12µg/ml at the λmax=267.68nm. Accuracy and precision studies were carried out and results were satisfactorily obtained. The drug at each of the 80 %, 100 % and 120 % levels showed good recoveries that is in the range of 98.00 to 99.00% for both methods, hence it could be said that the method was accurate. Limit of detection (LOD) and limit of quantitation (LOQ) were determined for the method. The method was validated as per International Conference on Harmonization. All validation parameters were within the acceptable limit. The developed method was successfully applied to estimate the amount of Olmutinib in pharmaceutical formulation.

scholarly journals Estimation of Levocetirizine in Bulk and Formulation by First Order Derivative Area under Curve UV-Spectrophotometric Methods.

2016 ◽  
Vol 2 (1) ◽  
pp. 09
Author(s):  
Pandurang Tukaram Mane
Keyword(s):  
Area Under Curve ◽  
Pharmaceutical Formulations ◽  
Order Derivative ◽  
Pharmaceutical Dosage Forms ◽  
Mean Values ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Significant Difference

Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 235-243 nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.9994) for first order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Levocetirizine in pharmaceutical formulations.

NOVEL ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF BROMOFENAC SODIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM BY DERIVATIVE AND AUC SPECTROSCOPIC METHOD

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (01) ◽  
pp. 87-90
Author(s):  
S Raghu ◽  
S. Muneer. ◽  
K.B. Chandra sekhar ◽  
kiran B. Siva Sai ◽  
Keyword(s):  
Spectrophotometric Method ◽  
Method Development ◽  
Spectroscopic Method ◽  
Zero Order ◽  
Order Derivative ◽  
Pharmaceutical Dosage Form ◽  
Pharmaceutical Dosage Forms ◽  
First Order ◽  
Significant Difference

The present study was carried out to develop a simple and reliable derivative spectrophotometric method for the determination of bromofenac sodium in pharmaceutical dosage forms .The solutions of standard and the sample were prepared in distilled water The quantitative determination of the drug was carried out with zero order derivative (method A) values measured at 268 nm and first order (method B) derivative values measured at 258 nm , and two wavelengths 262nm and 274nm were selected for the determination AUC (method C) of integrated areas. Calibration graphs constructed at their wavelengths of determination, were linear within the concentration range of 2-12 μg/mL (r2 = 0.999) for zero order and (r2 = 0.997) first order derivative spectrophotometric method. No significant difference between the performance of the proposed methods regarding the mean values and standard deviations and is suitable for the routine quality control application of bromofenac sodium in its pharmaceutical formulations.

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